Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signalling

Oyama, Kotaro; Ishii, Ayumi; Matsumura, Shuhei; Oyama, Tomoko G.; Taguchi, Mitsumasa; Suzuki, Madoka

doi:https://doi.org/10.1039/d5lc00675a

Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signalling

Oyama, Kotaro; Ishii, Ayumi; Matsumura, Shuhei et al.

Lab on a Chip, 2025, 25(22), 5863-5874

アクセス数:372件（2026-11-04 08:35 集計）

固定URL: https://hdl.handle.net/11094/105856

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タイトル	Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signalling
著者	NRID 1000070632131 1000070632131 著者名 Oyama, Kotaro
	NRID 1000070406833 1000070406833 著者名 Ishii, Ayumi
	著者名 Matsumura, Shuhei
	NRID 1000090717646 1000090717646 著者名 Oyama, Tomoko G.
	NRID 1000060343943 1000060343943 著者名 Taguchi, Mitsumasa
	NRID 1000040350475 1000040350475 ORCID 0000-0002-3270-1755 0000-0002-3270-1755 researchmap permalink read0097299 read0097299 Scopus Author ID 38060904300 38060904300 著者名 Suzuki, Madoka
内容	Oyama K., Ishii A., Matsumura S., et al. Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signalling. Lab on a Chip 25(22), 5863–5874 (2025); https://doi.org/10.1039/d5lc00675a.
抄録	Optical microheating technologies have revealed how biological systems sense heating and cooling at the microscopic scale. Sensing is based on thermosensitive biochemical reactions that frequently engage membrane proteins, Ca²⁺ channels, and pumps to convert sensing information as the Ca²⁺ signalling in cells. These findings highlight the feasibility of thermally manipulating intracellular Ca²⁺ signalling. However, how the thermosensitive Ca²⁺ signalling would behave, particularly in multicellular systems, remains elusive. In this study, to extend the ability of the spatiotemporal temperature control by optical microheating technologies, we propose holographic heating microscopy. Water-absorbable infrared (IR) laser light is modulated by a reflective liquid crystal on a silicon spatial light modulator (LCOS-SLM). A computer-generated hologram displayed on the LCOS-SLM modulates the spatial phase pattern of the IR laser light to generate predesigned temperature gradients at the microscope focal plane. The holographic heating microscopy visualises how thermosensitive Ca²⁺ signalling is generated and propagated in MDCK cells, rat hippocampal neurons, and rat neonatal cardiomyocytes. Moreover, the optical control of the temporal temperature gradient reveals the cooling-rate dependency of Ca²⁺ signalling in HeLa cells. These findings demonstrate the extended ability of holographic heating microscopy in investigating cellular thermosensitivities and thermally manipulating cellular functions.
出版者	Royal Society of Chemistry
収録物名	Lab on a Chip
巻(号)	25(22)
ページ	5863-5874
刊行年月	2025-10-06
言語	英語
ハンドルURL	https://hdl.handle.net/11094/105856
PISSN	14730197
eISSN	14730189
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1039/d5lc00675a
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Lab on a Chip. 2025, 25(22), p. 5863-5874
DC.title	Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signalling
DC.creator	Oyama, Kotaro
	Ishii, Ayumi
	Matsumura, Shuhei
	Oyama, Tomoko G.
	Taguchi, Mitsumasa
	Suzuki, Madoka
DC.publisher	Royal Society of Chemistry
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-10-06
DC.identifier	https://doi.org/10.1039/d5lc00675a
DC.description	Oyama K., Ishii A., Matsumura S., et al. Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signalling. Lab on a Chip 25(22), 5863–5874 (2025); https://doi.org/10.1039/d5lc00675a.
DCTERMS.abstract	Optical microheating technologies have revealed how biological systems sense heating and cooling at the microscopic scale. Sensing is based on thermosensitive biochemical reactions that frequently engage membrane proteins, Ca²⁺ channels, and pumps to convert sensing information as the Ca²⁺ signalling in cells. These findings highlight the feasibility of thermally manipulating intracellular Ca²⁺ signalling. However, how the thermosensitive Ca²⁺ signalling would behave, particularly in multicellular systems, remains elusive. In this study, to extend the ability of the spatiotemporal temperature control by optical microheating technologies, we propose holographic heating microscopy. Water-absorbable infrared (IR) laser light is modulated by a reflective liquid crystal on a silicon spatial light modulator (LCOS-SLM). A computer-generated hologram displayed on the LCOS-SLM modulates the spatial phase pattern of the IR laser light to generate predesigned temperature gradients at the microscope focal plane. The holographic heating microscopy visualises how thermosensitive Ca²⁺ signalling is generated and propagated in MDCK cells, rat hippocampal neurons, and rat neonatal cardiomyocytes. Moreover, the optical control of the temporal temperature gradient reveals the cooling-rate dependency of Ca²⁺ signalling in HeLa cells. These findings demonstrate the extended ability of holographic heating microscopy in investigating cellular thermosensitivities and thermally manipulating cellular functions.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	105856
citation_title	Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signalling
citation_author	Oyama, Kotaro
	Ishii, Ayumi
	Matsumura, Shuhei
	Oyama, Tomoko G.
	Taguchi, Mitsumasa
	Suzuki, Madoka
citation_publisher	Royal Society of Chemistry
citation_language	英語
citation_date	2025-10-06
citation_journal_title	Lab on a Chip
citation_volume	25
citation_issue	22
citation_firstpage	5863
citation_lastpage	5874
citation_doi	https://doi.org/10.1039/d5lc00675a
citation_public_url	https://hdl.handle.net/11094/105856

Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signalling

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