Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells

Sato, Miyuki; Saegusa, Keiko; Sato, Katsuya; Hara, Taichi; Harada, Akihiro; Sato, ken

doi:info:doi/10.1091/mbc.E11-04-0279

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タイトル	Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
著者	Sato, Miyuki Sato, Miyuki
	Saegusa, Keiko Saegusa, Keiko
	Sato, Katsuya Sato, Katsuya
	Hara, Taichi Hara, Taichi
	Harada, Akihiro Harada, Akihiro
	Sato, ken Sato, ken
抄録	It is generally accepted that soluble N-ethylmaleimide–sensitive factor attachment protein receptors mediate the docking and fusion of transport intermediates with target membranes. Our research identifies Caenorhabditis elegans homologue of synaptosomal-associated protein 29 (SNAP-29) as an essential regulator of membrane trafficking in polarized intestinal cells of living animals. We show that a depletion of SNAP-29 blocks yolk secretion and targeting of apical and basolateral plasma membrane proteins in the intestinal cells and results in a strong accumulation of small cargo-containing vesicles. The loss of SNAP-29 also blocks the transport of yolk receptor RME-2 to the plasma membrane in nonpolarized oocytes, indicating that its function is required in various cell types. SNAP-29 is essential for embryogenesis, animal growth, and viability. Functional fluorescent protein–tagged SNAP-29 mainly localizes to the plasma membrane and the late Golgi, although it also partially colocalizes with endosomal proteins. The loss of SNAP-29 leads to the vesiculation/fragmentation of the Golgi and endosomes, suggesting that SNAP-29 is involved in multiple transport pathways between the exocytic and endocytic organelles. These observations also suggest that organelles comprising the endomembrane system are highly dynamic structures based on the balance between membrane budding and fusion and that SNAP-29–mediated fusion is required to maintain proper organellar morphology and functions.
公開者	The American Society for Cell Biology
掲載誌名	Molecular Biology of the Cell
巻	22
号	14
開始ページ	2579
終了ページ	2587
刊行年月	2011-07-15
ISSN	10591524
NCID	AA10830484
DOI	info:doi/10.1091/mbc.E11-04-0279
URL	http://hdl.handle.net/11094/23086
関連情報 (references)	http://www.molbiolcell.org/content/22/14/2579.full
言語	英語

著者版フラグ	publisher
NII資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
dcmi資源タイプ	text
DCTERMS.bibliographicCitation	Molecular Biology of the Cell.22(14) P.2579-P.2587
DC.title	Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
DC.creator	Sato, Miyuki
	Saegusa, Keiko
	Sato, Katsuya
	Hara, Taichi
	Harada, Akihiro
	Sato, ken
DC.publisher	The American Society for Cell Biology
DC.language" scheme="DCTERMS.RFC1766	英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF	2011-07-15
DC.identifier	info:doi/10.1091/mbc.E11-04-0279
DC.identifier" scheme="DCTERMS.URI	http://hdl.handle.net/11094/23086
DCTERMS.abstract	It is generally accepted that soluble N-ethylmaleimide–sensitive factor attachment protein receptors mediate the docking and fusion of transport intermediates with target membranes. Our research identifies Caenorhabditis elegans homologue of synaptosomal-associated protein 29 (SNAP-29) as an essential regulator of membrane trafficking in polarized intestinal cells of living animals. We show that a depletion of SNAP-29 blocks yolk secretion and targeting of apical and basolateral plasma membrane proteins in the intestinal cells and results in a strong accumulation of small cargo-containing vesicles. The loss of SNAP-29 also blocks the transport of yolk receptor RME-2 to the plasma membrane in nonpolarized oocytes, indicating that its function is required in various cell types. SNAP-29 is essential for embryogenesis, animal growth, and viability. Functional fluorescent protein–tagged SNAP-29 mainly localizes to the plasma membrane and the late Golgi, although it also partially colocalizes with endosomal proteins. The loss of SNAP-29 leads to the vesiculation/fragmentation of the Golgi and endosomes, suggesting that SNAP-29 is involved in multiple transport pathways between the exocytic and endocytic organelles. These observations also suggest that organelles comprising the endomembrane system are highly dynamic structures based on the balance between membrane budding and fusion and that SNAP-29–mediated fusion is required to maintain proper organellar morphology and functions.
citation_title	Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
citation_author	Sato, Miyuki
	Saegusa, Keiko
	Sato, Katsuya
	Hara, Taichi
	Harada, Akihiro
	Sato, ken
citation_publisher	The American Society for Cell Biology
citation_language	英語
citation_date	2011-07-15
citation_journal_title	Molecular Biology of the Cell
citation_volume	22
citation_issue	14
citation_firstpage	2579
citation_lastpage	2587
citation_issn	10591524
citation_doi	info:doi/10.1091/mbc.E11-04-0279
citation_public_url	http://hdl.handle.net/11094/23086

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