Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells

Sato, Miyuki; Saegusa, Keiko; Sato, Katsuya; Hara, Taichi; Harada, Akihiro; Sato, Ken

doi:https://doi.org/10.1091/mbc.E11-04-0279

Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells

Sato, Miyuki; Saegusa, Keiko; Sato, Katsuya et al.

Molecular Biology of the Cell, 2011, 22(14), 2579-2587

アクセス数:773件（2025-07-12 04:38 集計）

固定URL: https://hdl.handle.net/11094/23086

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タイトル	Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
著者	NRID 1000070321768 1000070321768 著者名 Sato, Miyuki
	著者名 Saegusa, Keiko
	NRID 1000090370402 1000090370402 著者名 Sato, Katsuya
	NRID 1000000392374 1000000392374 著者名 Hara, Taichi
	NRID 1000040251441 1000040251441 researchmap permalink aharada aharada Scopus Author ID 7202253419 7202253419 著者名 Harada, Akihiro
	著者名 Sato, Ken
抄録	It is generally accepted that soluble N-ethylmaleimide–sensitive factor attachment protein receptors mediate the docking and fusion of transport intermediates with target membranes. Our research identifies Caenorhabditis elegans homologue of synaptosomal-associated protein 29 (SNAP-29) as an essential regulator of membrane trafficking in polarized intestinal cells of living animals. We show that a depletion of SNAP-29 blocks yolk secretion and targeting of apical and basolateral plasma membrane proteins in the intestinal cells and results in a strong accumulation of small cargo-containing vesicles. The loss of SNAP-29 also blocks the transport of yolk receptor RME-2 to the plasma membrane in nonpolarized oocytes, indicating that its function is required in various cell types. SNAP-29 is essential for embryogenesis, animal growth, and viability. Functional fluorescent protein–tagged SNAP-29 mainly localizes to the plasma membrane and the late Golgi, although it also partially colocalizes with endosomal proteins. The loss of SNAP-29 leads to the vesiculation/fragmentation of the Golgi and endosomes, suggesting that SNAP-29 is involved in multiple transport pathways between the exocytic and endocytic organelles. These observations also suggest that organelles comprising the endomembrane system are highly dynamic structures based on the balance between membrane budding and fusion and that SNAP-29–mediated fusion is required to maintain proper organellar morphology and functions.
出版者	The American Society for Cell Biology
収録物名	Molecular Biology of the Cell
巻(号)	22(14)
ページ	2579-2587
刊行年月	2011-07-15
言語	英語
ハンドルURL	https://hdl.handle.net/11094/23086
PISSN	10591524
NCID	AA10830484
関連情報 (references)	URI http://www.molbiolcell.org/content/22/14/2579.full
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1091/mbc.E11-04-0279
アクセス権	open access
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Molecular Biology of the Cell. 2011, 22(14), p. 2579-2587
DC.title	Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
DC.creator	Sato, Miyuki
	Saegusa, Keiko
	Sato, Katsuya
	Hara, Taichi
	Harada, Akihiro
	Sato, Ken
DC.publisher	The American Society for Cell Biology
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2011-07-15
DC.identifier	https://doi.org/10.1091/mbc.E11-04-0279
DC.relation' scheme='DCTERMS.URI	http://www.molbiolcell.org/content/22/14/2579.full
DCTERMS.abstract	It is generally accepted that soluble N-ethylmaleimide–sensitive factor attachment protein receptors mediate the docking and fusion of transport intermediates with target membranes. Our research identifies Caenorhabditis elegans homologue of synaptosomal-associated protein 29 (SNAP-29) as an essential regulator of membrane trafficking in polarized intestinal cells of living animals. We show that a depletion of SNAP-29 blocks yolk secretion and targeting of apical and basolateral plasma membrane proteins in the intestinal cells and results in a strong accumulation of small cargo-containing vesicles. The loss of SNAP-29 also blocks the transport of yolk receptor RME-2 to the plasma membrane in nonpolarized oocytes, indicating that its function is required in various cell types. SNAP-29 is essential for embryogenesis, animal growth, and viability. Functional fluorescent protein–tagged SNAP-29 mainly localizes to the plasma membrane and the late Golgi, although it also partially colocalizes with endosomal proteins. The loss of SNAP-29 leads to the vesiculation/fragmentation of the Golgi and endosomes, suggesting that SNAP-29 is involved in multiple transport pathways between the exocytic and endocytic organelles. These observations also suggest that organelles comprising the endomembrane system are highly dynamic structures based on the balance between membrane budding and fusion and that SNAP-29–mediated fusion is required to maintain proper organellar morphology and functions.
DC.format	23086
citation_title	Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
citation_author	Sato, Miyuki
	Saegusa, Keiko
	Sato, Katsuya
	Hara, Taichi
	Harada, Akihiro
	Sato, Ken
citation_publisher	The American Society for Cell Biology
citation_language	英語
citation_date	2011-07-15
citation_journal_title	Molecular Biology of the Cell
citation_volume	22
citation_issue	14
citation_firstpage	2579
citation_lastpage	2587
citation_doi	https://doi.org/10.1091/mbc.E11-04-0279
citation_public_url	https://hdl.handle.net/11094/23086
citation_abstract_html_url	http://www.molbiolcell.org/content/22/14/2579.full

Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells

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