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https://doi.org/10.1091/mbc.E11-04-0279
このアイテムへのリンクには次のURLをご利用ください:http://hdl.handle.net/11094/23086
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論文情報
タイトル
Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
著者
Sato, Miyuki
Sato, Miyuki
Saegusa, Keiko
Saegusa, Keiko
Sato, Katsuya
Sato, Katsuya
Hara, Taichi
Hara, Taichi
Harada, Akihiro
Harada, Akihiro
Sato, ken
Sato, ken
抄録
It is generally accepted that soluble N-ethylmaleimide–sensitive factor attachment protein receptors mediate the docking and fusion of transport intermediates with target membranes. Our research identifies Caenorhabditis elegans homologue of synaptosomal-associated protein 29 (SNAP-29) as an essential regulator of membrane trafficking in polarized intestinal cells of living animals. We show that a depletion of SNAP-29 blocks yolk secretion and targeting of apical and basolateral plasma membrane proteins in the intestinal cells and results in a strong accumulation of small cargo-containing vesicles. The loss of SNAP-29 also blocks the transport of yolk receptor RME-2 to the plasma membrane in nonpolarized oocytes, indicating that its function is required in various cell types. SNAP-29 is essential for embryogenesis, animal growth, and viability. Functional fluorescent protein–tagged SNAP-29 mainly localizes to the plasma membrane and the late Golgi, although it also partially colocalizes with endosomal proteins. The loss of SNAP-29 leads to the vesiculation/fragmentation of the Golgi and endosomes, suggesting that SNAP-29 is involved in multiple transport pathways between the exocytic and endocytic organelles. These observations also suggest that organelles comprising the endomembrane system are highly dynamic structures based on the balance between membrane budding and fusion and that SNAP-29–mediated fusion is required to maintain proper organellar morphology and functions.
公開者
The American Society for Cell Biology
掲載誌名
Molecular Biology of the Cell
巻
22
号
14
開始ページ
2579
終了ページ
2587
刊行年月
2011-07-15
ISSN
10591524
NCID
AA10830484
DOI
info:doi/10.1091/mbc.E11-04-0279
URL
http://hdl.handle.net/11094/23086
関連情報 (references)
http://www.molbiolcell.org/content/22/14/2579.full
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英語
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学術雑誌論文 Journal Article
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DCTERMS.bibliographicCitation
Molecular Biology of the Cell.22(14) P.2579-P.2587
DC.title
Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
DC.creator
Sato, Miyuki
Saegusa, Keiko
Sato, Katsuya
Hara, Taichi
Harada, Akihiro
Sato, ken
DC.publisher
The American Society for Cell Biology
DC.language" scheme="DCTERMS.RFC1766
英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF
2011-07-15
DC.identifier
info:doi/10.1091/mbc.E11-04-0279
DC.identifier" scheme="DCTERMS.URI
http://hdl.handle.net/11094/23086
DCTERMS.abstract
It is generally accepted that soluble N-ethylmaleimide–sensitive factor attachment protein receptors mediate the docking and fusion of transport intermediates with target membranes. Our research identifies Caenorhabditis elegans homologue of synaptosomal-associated protein 29 (SNAP-29) as an essential regulator of membrane trafficking in polarized intestinal cells of living animals. We show that a depletion of SNAP-29 blocks yolk secretion and targeting of apical and basolateral plasma membrane proteins in the intestinal cells and results in a strong accumulation of small cargo-containing vesicles. The loss of SNAP-29 also blocks the transport of yolk receptor RME-2 to the plasma membrane in nonpolarized oocytes, indicating that its function is required in various cell types. SNAP-29 is essential for embryogenesis, animal growth, and viability. Functional fluorescent protein–tagged SNAP-29 mainly localizes to the plasma membrane and the late Golgi, although it also partially colocalizes with endosomal proteins. The loss of SNAP-29 leads to the vesiculation/fragmentation of the Golgi and endosomes, suggesting that SNAP-29 is involved in multiple transport pathways between the exocytic and endocytic organelles. These observations also suggest that organelles comprising the endomembrane system are highly dynamic structures based on the balance between membrane budding and fusion and that SNAP-29–mediated fusion is required to maintain proper organellar morphology and functions.
citation_title
Caenorhabditis elegans SNAP-29 is required for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells
citation_author
Sato, Miyuki
Saegusa, Keiko
Sato, Katsuya
Hara, Taichi
Harada, Akihiro
Sato, ken
citation_publisher
The American Society for Cell Biology
citation_language
英語
citation_date
2011-07-15
citation_journal_title
Molecular Biology of the Cell
citation_volume
22
citation_issue
14
citation_firstpage
2579
citation_lastpage
2587
citation_issn
10591524
citation_doi
info:doi/10.1091/mbc.E11-04-0279
citation_public_url
http://hdl.handle.net/11094/23086