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https://doi.org/10.1128/MCB.01089-08
このアイテムへのリンクには次のURLをご利用ください:http://hdl.handle.net/11094/23088
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タイトル
p31 Deficiency Influences Endoplasmic Reticulum Tubular Morphology and Cell Survival
著者
Uemura, Takefumi
Uemura, Takefumi
Sato, Takashi
Sato, Takashi
Aoki, Takehiro
Aoki, Takehiro
Yamamoto, Akitsugu
Yamamoto, Akitsugu
Okada, Tetsuya
Okada, Tetsuya
Hirai, Rika
Hirai, Rika
Harada, Reiko
Harada, Reiko
Mori, Kazutoshi
Mori, Kazutoshi
Tagaya, Mitsuo
Tagaya, Mitsuo
Harada, Akihiro
Harada, Akihiro
抄録
p31, the mammalian orthologue of yeast Use1p, is an endoplasmic reticulum (ER)-localized soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) that forms a complex with other SNAREs, particularly syntaxin 18. However, the role of p31 in ER function remains unknown. To determine the role of p31 in vivo, we generated p31 conditional knockout mice. We found that homozygous deletion of the p31 gene led to early embryonic lethality before embryonic day 8.5. Conditional knockout of p31 in brains and mouse embryonic fibroblasts (MEFs) caused massive apoptosis accompanied by upregulation of ER stress-associated genes. Microscopic analysis showed vesiculation and subsequent enlargement of the ER membrane in p31-deficient cells. This type of drastic disorganization in the ER tubules has not been demonstrated to date. This marked change in ER structure preceded nuclear translocation of the ER stress-related transcription factor C/EBP homologous protein (CHOP), suggesting that ER stress-induced apoptosis resulted from disruption of the ER membrane structure. Taken together, these results suggest that p31 is an essential molecule involved in the maintenance of ER morphology and that its deficiency leads to ER stress-induced apoptosis.
公開者
American Society for Microbiology
掲載誌名
Molecular and Cellular Biology
巻
29
号
7
開始ページ
1869
終了ページ
1881
刊行年月
2009-04
ISSN
02707306
NCID
AA10620925
DOI
info:doi/10.1128/MCB.01089-08
URL
http://hdl.handle.net/11094/23088
言語
英語
カテゴリ
学術雑誌論文 Journal Article
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DCTERMS.bibliographicCitation
Molecular and Cellular Biology.29(7) P.1869-P.1881
DC.title
p31 Deficiency Influences Endoplasmic Reticulum Tubular Morphology and Cell Survival
DC.creator
Uemura, Takefumi
Sato, Takashi
Aoki, Takehiro
Yamamoto, Akitsugu
Okada, Tetsuya
Hirai, Rika
Harada, Reiko
Mori, Kazutoshi
Tagaya, Mitsuo
Harada, Akihiro
DC.publisher
American Society for Microbiology
DC.language" scheme="DCTERMS.RFC1766
英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF
2009-04
DC.identifier
info:doi/10.1128/MCB.01089-08
DC.identifier" scheme="DCTERMS.URI
http://hdl.handle.net/11094/23088
DCTERMS.abstract
p31, the mammalian orthologue of yeast Use1p, is an endoplasmic reticulum (ER)-localized soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) that forms a complex with other SNAREs, particularly syntaxin 18. However, the role of p31 in ER function remains unknown. To determine the role of p31 in vivo, we generated p31 conditional knockout mice. We found that homozygous deletion of the p31 gene led to early embryonic lethality before embryonic day 8.5. Conditional knockout of p31 in brains and mouse embryonic fibroblasts (MEFs) caused massive apoptosis accompanied by upregulation of ER stress-associated genes. Microscopic analysis showed vesiculation and subsequent enlargement of the ER membrane in p31-deficient cells. This type of drastic disorganization in the ER tubules has not been demonstrated to date. This marked change in ER structure preceded nuclear translocation of the ER stress-related transcription factor C/EBP homologous protein (CHOP), suggesting that ER stress-induced apoptosis resulted from disruption of the ER membrane structure. Taken together, these results suggest that p31 is an essential molecule involved in the maintenance of ER morphology and that its deficiency leads to ER stress-induced apoptosis.
citation_title
p31 Deficiency Influences Endoplasmic Reticulum Tubular Morphology and Cell Survival
citation_author
Uemura, Takefumi
Sato, Takashi
Aoki, Takehiro
Yamamoto, Akitsugu
Okada, Tetsuya
Hirai, Rika
Harada, Reiko
Mori, Kazutoshi
Tagaya, Mitsuo
Harada, Akihiro
citation_publisher
American Society for Microbiology
citation_language
英語
citation_date
2009-04
citation_journal_title
Molecular and Cellular Biology
citation_volume
29
citation_issue
7
citation_firstpage
1869
citation_lastpage
1881
citation_issn
02707306
citation_doi
info:doi/10.1128/MCB.01089-08
citation_public_url
http://hdl.handle.net/11094/23088