EXPRESSION AND FUNCTION OF A NONGLYCOSYLATED MAJOR HISTOCOMPATIBILITY CLASS I ANTIGEN

Miyazaki, Jun-ichi; Appella, Ettore; Zhao, Hong; Forman, James; Ozato, Keiko

doi:info:doi/10.1084/jem.163.4.856

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タイトル	EXPRESSION AND FUNCTION OF A NONGLYCOSYLATED MAJOR HISTOCOMPATIBILITY CLASS I ANTIGEN
著者	Miyazaki, Jun-ichi Miyazaki, Jun-ichi
	Appella, Ettore Appella, Ettore
	Zhao, Hong Zhao, Hong
	Forman, James Forman, James
	Ozato, Keiko Ozato, Keiko
抄録	The major histocompatibility class I antigens, expressed in most somatic cells, have carbohydrate moieties. We constructed mutant mouse MHC class I genes in which codons for the N-linked glycosylation sites were replaced by those of other amino acids. L cell transformants expressing the nonglycosylated class I antigens allowed us to investigate biological roles of carbohydrates with the highest specificity possible. The nonglycosylated antigen was unchanged in its overall serological specificities, and was recognized by alloreactive cytotoxic T cells. Further, the antigen was capable of mediating cytotoxic activity of vesicular stomatitis virus-specific T cells. These studies indicate that carbohydrates are not essential for immunological function of the MHC class I antigens. Cell surface expression of the nonglycosylated antigen was markedly reduced as compared with the native antigen, which was not attributable to accelerated degradation or rapid shedding. We conclude that the primary role of carbohydrates of the class I antigens is to facilitate the intracellular transport of the nascent proteins to the plasma membrane. The possible involvement of carbohydrate-receptor interactions in this process is discussed.
公開者	The Rockefeller University Press
掲載誌名	Journal of Experimental Medicine
巻	163
号	4
開始ページ	856
終了ページ	871
刊行年月	1986-04-01
ISSN	00221007
NCID	AA00697559
DOI (出版社版)	info:doi/10.1084/jem.163.4.856
URL	http://hdl.handle.net/11094/23105
権利情報	© 1986 Rockefeller University Press
言語	英語
カテゴリ	学術雑誌論文 Journal Article

著者版フラグ	publisher
NII資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
dcmi資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Experimental Medicine.163(4) P.856-P.871
DC.title	EXPRESSION AND FUNCTION OF A NONGLYCOSYLATED MAJOR HISTOCOMPATIBILITY CLASS I ANTIGEN
DC.creator	Miyazaki, Jun-ichi
	Appella, Ettore
	Zhao, Hong
	Forman, James
	Ozato, Keiko
DC.publisher	The Rockefeller University Press
DC.language" scheme="DCTERMS.RFC1766	英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF	1986-04-01
DC.identifier	info:doi/10.1084/jem.163.4.856
DC.identifier" scheme="DCTERMS.URI	http://hdl.handle.net/11094/23105
DCTERMS.abstract	The major histocompatibility class I antigens, expressed in most somatic cells, have carbohydrate moieties. We constructed mutant mouse MHC class I genes in which codons for the N-linked glycosylation sites were replaced by those of other amino acids. L cell transformants expressing the nonglycosylated class I antigens allowed us to investigate biological roles of carbohydrates with the highest specificity possible. The nonglycosylated antigen was unchanged in its overall serological specificities, and was recognized by alloreactive cytotoxic T cells. Further, the antigen was capable of mediating cytotoxic activity of vesicular stomatitis virus-specific T cells. These studies indicate that carbohydrates are not essential for immunological function of the MHC class I antigens. Cell surface expression of the nonglycosylated antigen was markedly reduced as compared with the native antigen, which was not attributable to accelerated degradation or rapid shedding. We conclude that the primary role of carbohydrates of the class I antigens is to facilitate the intracellular transport of the nascent proteins to the plasma membrane. The possible involvement of carbohydrate-receptor interactions in this process is discussed.
DC.rights	© 1986 Rockefeller University Press
citation_title	EXPRESSION AND FUNCTION OF A NONGLYCOSYLATED MAJOR HISTOCOMPATIBILITY CLASS I ANTIGEN
citation_author	Miyazaki, Jun-ichi
	Appella, Ettore
	Zhao, Hong
	Forman, James
	Ozato, Keiko
citation_publisher	The Rockefeller University Press
citation_language	英語
citation_date	1986-04-01
citation_journal_title	Journal of Experimental Medicine
citation_volume	163
citation_issue	4
citation_firstpage	856
citation_lastpage	871
citation_issn	00221007
citation_doi	info:doi/10.1084/jem.163.4.856
citation_public_url	http://hdl.handle.net/11094/23105

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