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2021-01-23
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このアイテムへのリンクには次のURLをご利用ください:
https://doi.org/10.1084/jem.163.4.856
このアイテムへのリンクには次のURLをご利用ください:http://hdl.handle.net/11094/23105
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jem_163_04_0856
pdf
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論文情報
タイトル
EXPRESSION AND FUNCTION OF A NONGLYCOSYLATED MAJOR HISTOCOMPATIBILITY CLASS I ANTIGEN
著者
Miyazaki, Jun-ichi
Miyazaki, Jun-ichi
Appella, Ettore
Appella, Ettore
Zhao, Hong
Zhao, Hong
Forman, James
Forman, James
Ozato, Keiko
Ozato, Keiko
抄録
The major histocompatibility class I antigens, expressed in most somatic cells, have carbohydrate moieties. We constructed mutant mouse MHC class I genes in which codons for the N-linked glycosylation sites were replaced by those of other amino acids. L cell transformants expressing the nonglycosylated class I antigens allowed us to investigate biological roles of carbohydrates with the highest specificity possible. The nonglycosylated antigen was unchanged in its overall serological specificities, and was recognized by alloreactive cytotoxic T cells. Further, the antigen was capable of mediating cytotoxic activity of vesicular stomatitis virus-specific T cells. These studies indicate that carbohydrates are not essential for immunological function of the MHC class I antigens. Cell surface expression of the nonglycosylated antigen was markedly reduced as compared with the native antigen, which was not attributable to accelerated degradation or rapid shedding. We conclude that the primary role of carbohydrates of the class I antigens is to facilitate the intracellular transport of the nascent proteins to the plasma membrane. The possible involvement of carbohydrate-receptor interactions in this process is discussed.
公開者
The Rockefeller University Press
掲載誌名
Journal of Experimental Medicine
巻
163
号
4
開始ページ
856
終了ページ
871
刊行年月
1986-04-01
ISSN
00221007
NCID
AA00697559
DOI
info:doi/10.1084/jem.163.4.856
URL
http://hdl.handle.net/11094/23105
権利情報
© 1986 Rockefeller University Press
言語
英語
カテゴリ
学術雑誌論文 Journal Article
論文詳細を表示
著者版フラグ
publisher
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学術雑誌論文
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text
DCTERMS.bibliographicCitation
Journal of Experimental Medicine.163(4) P.856-P.871
DC.title
EXPRESSION AND FUNCTION OF A NONGLYCOSYLATED MAJOR HISTOCOMPATIBILITY CLASS I ANTIGEN
DC.creator
Miyazaki, Jun-ichi
Appella, Ettore
Zhao, Hong
Forman, James
Ozato, Keiko
DC.publisher
The Rockefeller University Press
DC.language" scheme="DCTERMS.RFC1766
英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF
1986-04-01
DC.identifier
info:doi/10.1084/jem.163.4.856
DC.identifier" scheme="DCTERMS.URI
http://hdl.handle.net/11094/23105
DCTERMS.abstract
The major histocompatibility class I antigens, expressed in most somatic cells, have carbohydrate moieties. We constructed mutant mouse MHC class I genes in which codons for the N-linked glycosylation sites were replaced by those of other amino acids. L cell transformants expressing the nonglycosylated class I antigens allowed us to investigate biological roles of carbohydrates with the highest specificity possible. The nonglycosylated antigen was unchanged in its overall serological specificities, and was recognized by alloreactive cytotoxic T cells. Further, the antigen was capable of mediating cytotoxic activity of vesicular stomatitis virus-specific T cells. These studies indicate that carbohydrates are not essential for immunological function of the MHC class I antigens. Cell surface expression of the nonglycosylated antigen was markedly reduced as compared with the native antigen, which was not attributable to accelerated degradation or rapid shedding. We conclude that the primary role of carbohydrates of the class I antigens is to facilitate the intracellular transport of the nascent proteins to the plasma membrane. The possible involvement of carbohydrate-receptor interactions in this process is discussed.
DC.rights
© 1986 Rockefeller University Press
citation_title
EXPRESSION AND FUNCTION OF A NONGLYCOSYLATED MAJOR HISTOCOMPATIBILITY CLASS I ANTIGEN
citation_author
Miyazaki, Jun-ichi
Appella, Ettore
Zhao, Hong
Forman, James
Ozato, Keiko
citation_publisher
The Rockefeller University Press
citation_language
英語
citation_date
1986-04-01
citation_journal_title
Journal of Experimental Medicine
citation_volume
163
citation_issue
4
citation_firstpage
856
citation_lastpage
871
citation_issn
00221007
citation_doi
info:doi/10.1084/jem.163.4.856
citation_public_url
http://hdl.handle.net/11094/23105