Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction

Komamura, Kazuo; Tatsumi, Rie; Tsujita-Kuroda, Yuko; Onoe, Takatoshi; Matsumoto, kunio; Nakamura, Toshikazu; Miyazaki, Jun-ichi; Horio, Takeshi; Sugimachi, Masaru

doi:https://doi.org/10.1155/2011/453619

このアイテムのアクセス数:185件（2022-10-02 05:28 集計）

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タイトル	Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
著者	NRID 1000090311448 1000090311448 著者名 Komamura, Kazuo
	NRID 1000040584727 1000040584727 著者名 Tatsumi, Rie
	著者名 Tsujita-Kuroda, Yuko
	NRID 1000090098012 1000090098012 著者名 Onoe, Takatoshi
	NRID 1000090201780 1000090201780 著者名 Matsumoto, kunio
	NRID 1000000049397 1000000049397 著者名 Nakamura, Toshikazu
	NRID 1000080229830 1000080229830 著者名 Miyazaki, Jun-ichi
	NRID 1000050393228 1000050393228 著者名 Horio, Takeshi
	NRID 1000040250261 1000040250261 著者名 Sugimachi, Masaru
抄録	We transfected naked HGF plasmid DNA into cultured cardiomyocytes using a sonoporation method consisting of ultrasound-triggered bubble liposome destruction. We examined the effects on transfection efficiency of three concentrations of bubble liposome (1×10^6, 1×10^7, 1×10^8 μg/mL), three concentrations of HGF DNA (60, 120, 180 μg/mL), two insonification times (30, 60 sec), and three incubation times (15, 60, 120 min). We found that low concentrations of bubble liposome and low concentrations of DNA provided the largest amount of the HGF protein expression by the sonoporated cardiomyocytes. Variation of insonification and incubation times did not affect the amount of product. Following insonification, cardiomyocytes showed cellular injury, as determined by a dye exclusion test. The extent of injury was most severe with the highest concentration of bubble liposome. In conclusion, there are some trade-offs between gene transfection efficiency and cellular injury using ultrasound-triggered bubble liposome destruction as a method for gene transfection.
出版者	Hindawi Publishing Corporation
収録物名	Journal of Drug Delivery
巻	2011
号	453619
開始ページ	1
終了ページ	8
刊行年月	2011
言語	英語
ハンドルURL	https://hdl.handle.net/11094/23108
ISSN	20903014
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1155/2011/453619
アクセス権	open access
権利情報	Copyright © 2011 Kazuo Komamura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Drug Delivery.2011(453619) p.1-p.8
DC.title	Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
DC.creator	Komamura, Kazuo
	Tatsumi, Rie
	Tsujita-Kuroda, Yuko
	Onoe, Takatoshi
	Matsumoto, kunio
	Nakamura, Toshikazu
	Miyazaki, Jun-ichi
	Horio, Takeshi
	Sugimachi, Masaru
DC.publisher	Hindawi Publishing Corporation
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2011
DC.identifier	https://doi.org/10.1155/2011/453619
DCTERMS.abstract	We transfected naked HGF plasmid DNA into cultured cardiomyocytes using a sonoporation method consisting of ultrasound-triggered bubble liposome destruction. We examined the effects on transfection efficiency of three concentrations of bubble liposome (1×10^6, 1×10^7, 1×10^8 μg/mL), three concentrations of HGF DNA (60, 120, 180 μg/mL), two insonification times (30, 60 sec), and three incubation times (15, 60, 120 min). We found that low concentrations of bubble liposome and low concentrations of DNA provided the largest amount of the HGF protein expression by the sonoporated cardiomyocytes. Variation of insonification and incubation times did not affect the amount of product. Following insonification, cardiomyocytes showed cellular injury, as determined by a dye exclusion test. The extent of injury was most severe with the highest concentration of bubble liposome. In conclusion, there are some trade-offs between gene transfection efficiency and cellular injury using ultrasound-triggered bubble liposome destruction as a method for gene transfection.
DC.rights	Copyright © 2011 Kazuo Komamura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DC.format	application/pdf
citation_title	Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
citation_author	Komamura, Kazuo
	Tatsumi, Rie
	Tsujita-Kuroda, Yuko
	Onoe, Takatoshi
	Matsumoto, kunio
	Nakamura, Toshikazu
	Miyazaki, Jun-ichi
	Horio, Takeshi
	Sugimachi, Masaru
citation_publisher	Hindawi Publishing Corporation
citation_language	英語
citation_date	2011
citation_journal_title	Journal of Drug Delivery
citation_volume	2011
citation_issue	453619
citation_firstpage	1
citation_lastpage	8
citation_issn	20903014
citation_doi	https://doi.org/10.1155/2011/453619
citation_public_url	https://hdl.handle.net/11094/23108

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