ようこそ ゲスト さん
雑誌ブラウズ
雑誌 全て
大阪大学 刊行物
ランキング
アクセスランキング
ダウンロードランキング
博士論文のみをさがす
このアイテムのアクセス数:
132
件
(
2021-01-24
15:46 集計
)
このアイテムへのリンクには次のURLをご利用ください:
https://doi.org/10.1155/2011/453619
このアイテムへのリンクには次のURLをご利用ください:http://hdl.handle.net/11094/23108
閲覧可能ファイル
ファイル
フォーマット
サイズ
閲覧回数
説明
jdd_2011_453619_001
pdf
2.69 MB
177
論文情報
タイトル
Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
著者
Komamura, Kazuo
Komamura, Kazuo
Tatsumi, Rie
Tatsumi, Rie
Tsujita-Kuroda, Yuko
Tsujita-Kuroda, Yuko
Onoe, Takatoshi
Onoe, Takatoshi
Matsumoto, kunio
Matsumoto, kunio
Nakamura, Toshikazu
Nakamura, Toshikazu
Miyazaki, Jun-ichi
Miyazaki, Jun-ichi
Horio, Takeshi
Horio, Takeshi
Sugimachi, Masaru
Sugimachi, Masaru
抄録
We transfected naked HGF plasmid DNA into cultured cardiomyocytes using a sonoporation method consisting of ultrasound-triggered bubble liposome destruction. We examined the effects on transfection efficiency of three concentrations of bubble liposome (1×10^6, 1×10^7, 1×10^8 μg/mL), three concentrations of HGF DNA (60, 120, 180 μg/mL), two insonification times (30, 60 sec), and three incubation times (15, 60, 120 min). We found that low concentrations of bubble liposome and low concentrations of DNA provided the largest amount of the HGF protein expression by the sonoporated cardiomyocytes. Variation of insonification and incubation times did not affect the amount of product. Following insonification, cardiomyocytes showed cellular injury, as determined by a dye exclusion test. The extent of injury was most severe with the highest concentration of bubble liposome. In conclusion, there are some trade-offs between gene transfection efficiency and cellular injury using ultrasound-triggered bubble liposome destruction as a method for gene transfection.
公開者
Hindawi Publishing Corporation
掲載誌名
Journal of Drug Delivery
巻
2011
号
453619
開始ページ
1
終了ページ
8
刊行年月
2011
ISSN
20903014
DOI
info:doi/10.1155/2011/453619
URL
http://hdl.handle.net/11094/23108
権利情報
Copyright © 2011 Kazuo Komamura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
言語
英語
カテゴリ
学術雑誌論文 Journal Article
論文詳細を表示
著者版フラグ
publisher
NII資源タイプ
学術雑誌論文
ローカル資源タイプ
学術雑誌論文
dcmi資源タイプ
text
DCTERMS.bibliographicCitation
Journal of Drug Delivery.2011(453619) P.1-P.8
DC.title
Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
DC.creator
Komamura, Kazuo
Tatsumi, Rie
Tsujita-Kuroda, Yuko
Onoe, Takatoshi
Matsumoto, kunio
Nakamura, Toshikazu
Miyazaki, Jun-ichi
Horio, Takeshi
Sugimachi, Masaru
DC.publisher
Hindawi Publishing Corporation
DC.language" scheme="DCTERMS.RFC1766
英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF
2011
DC.identifier
info:doi/10.1155/2011/453619
DC.identifier" scheme="DCTERMS.URI
http://hdl.handle.net/11094/23108
DCTERMS.abstract
We transfected naked HGF plasmid DNA into cultured cardiomyocytes using a sonoporation method consisting of ultrasound-triggered bubble liposome destruction. We examined the effects on transfection efficiency of three concentrations of bubble liposome (1×10^6, 1×10^7, 1×10^8 μg/mL), three concentrations of HGF DNA (60, 120, 180 μg/mL), two insonification times (30, 60 sec), and three incubation times (15, 60, 120 min). We found that low concentrations of bubble liposome and low concentrations of DNA provided the largest amount of the HGF protein expression by the sonoporated cardiomyocytes. Variation of insonification and incubation times did not affect the amount of product. Following insonification, cardiomyocytes showed cellular injury, as determined by a dye exclusion test. The extent of injury was most severe with the highest concentration of bubble liposome. In conclusion, there are some trade-offs between gene transfection efficiency and cellular injury using ultrasound-triggered bubble liposome destruction as a method for gene transfection.
DC.rights
Copyright © 2011 Kazuo Komamura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
citation_title
Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
citation_author
Komamura, Kazuo
Tatsumi, Rie
Tsujita-Kuroda, Yuko
Onoe, Takatoshi
Matsumoto, kunio
Nakamura, Toshikazu
Miyazaki, Jun-ichi
Horio, Takeshi
Sugimachi, Masaru
citation_publisher
Hindawi Publishing Corporation
citation_language
英語
citation_date
2011
citation_journal_title
Journal of Drug Delivery
citation_volume
2011
citation_issue
453619
citation_firstpage
1
citation_lastpage
8
citation_issn
20903014
citation_doi
info:doi/10.1155/2011/453619
citation_public_url
http://hdl.handle.net/11094/23108