Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction

Komamura, Kazuo; Tatsumi, Rie; Tsujita-Kuroda, Yuko; Onoe, Takatoshi; Matsumoto, kunio; Nakamura, Toshikazu; Miyazaki, Jun-ichi; Horio, Takeshi; Sugimachi, Masaru

doi:info:doi/10.1155/2011/453619

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タイトル	Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
著者	Komamura, Kazuo Komamura, Kazuo
	Tatsumi, Rie Tatsumi, Rie
	Tsujita-Kuroda, Yuko Tsujita-Kuroda, Yuko
	Onoe, Takatoshi Onoe, Takatoshi
	Matsumoto, kunio Matsumoto, kunio
	Nakamura, Toshikazu Nakamura, Toshikazu
	Miyazaki, Jun-ichi Miyazaki, Jun-ichi
	Horio, Takeshi Horio, Takeshi
	Sugimachi, Masaru Sugimachi, Masaru
抄録	We transfected naked HGF plasmid DNA into cultured cardiomyocytes using a sonoporation method consisting of ultrasound-triggered bubble liposome destruction. We examined the effects on transfection efficiency of three concentrations of bubble liposome (1×10^6, 1×10^7, 1×10^8 μg/mL), three concentrations of HGF DNA (60, 120, 180 μg/mL), two insonification times (30, 60 sec), and three incubation times (15, 60, 120 min). We found that low concentrations of bubble liposome and low concentrations of DNA provided the largest amount of the HGF protein expression by the sonoporated cardiomyocytes. Variation of insonification and incubation times did not affect the amount of product. Following insonification, cardiomyocytes showed cellular injury, as determined by a dye exclusion test. The extent of injury was most severe with the highest concentration of bubble liposome. In conclusion, there are some trade-offs between gene transfection efficiency and cellular injury using ultrasound-triggered bubble liposome destruction as a method for gene transfection.
公開者	Hindawi Publishing Corporation
掲載誌名	Journal of Drug Delivery
巻	2011
号	453619
開始ページ	1
終了ページ	8
刊行年月	2011
ISSN	20903014
DOI (出版社版)	info:doi/10.1155/2011/453619
URL	http://hdl.handle.net/11094/23108
権利情報	Copyright © 2011 Kazuo Komamura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
言語	英語
カテゴリ	学術雑誌論文 Journal Article

著者版フラグ	publisher
NII資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
dcmi資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Drug Delivery.2011(453619) P.1-P.8
DC.title	Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
DC.creator	Komamura, Kazuo
	Tatsumi, Rie
	Tsujita-Kuroda, Yuko
	Onoe, Takatoshi
	Matsumoto, kunio
	Nakamura, Toshikazu
	Miyazaki, Jun-ichi
	Horio, Takeshi
	Sugimachi, Masaru
DC.publisher	Hindawi Publishing Corporation
DC.language" scheme="DCTERMS.RFC1766	英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF	2011
DC.identifier	info:doi/10.1155/2011/453619
DC.identifier" scheme="DCTERMS.URI	http://hdl.handle.net/11094/23108
DCTERMS.abstract	We transfected naked HGF plasmid DNA into cultured cardiomyocytes using a sonoporation method consisting of ultrasound-triggered bubble liposome destruction. We examined the effects on transfection efficiency of three concentrations of bubble liposome (1×10^6, 1×10^7, 1×10^8 μg/mL), three concentrations of HGF DNA (60, 120, 180 μg/mL), two insonification times (30, 60 sec), and three incubation times (15, 60, 120 min). We found that low concentrations of bubble liposome and low concentrations of DNA provided the largest amount of the HGF protein expression by the sonoporated cardiomyocytes. Variation of insonification and incubation times did not affect the amount of product. Following insonification, cardiomyocytes showed cellular injury, as determined by a dye exclusion test. The extent of injury was most severe with the highest concentration of bubble liposome. In conclusion, there are some trade-offs between gene transfection efficiency and cellular injury using ultrasound-triggered bubble liposome destruction as a method for gene transfection.
DC.rights	Copyright © 2011 Kazuo Komamura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
citation_title	Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
citation_author	Komamura, Kazuo
	Tatsumi, Rie
	Tsujita-Kuroda, Yuko
	Onoe, Takatoshi
	Matsumoto, kunio
	Nakamura, Toshikazu
	Miyazaki, Jun-ichi
	Horio, Takeshi
	Sugimachi, Masaru
citation_publisher	Hindawi Publishing Corporation
citation_language	英語
citation_date	2011
citation_journal_title	Journal of Drug Delivery
citation_volume	2011
citation_issue	453619
citation_firstpage	1
citation_lastpage	8
citation_issn	20903014
citation_doi	info:doi/10.1155/2011/453619
citation_public_url	http://hdl.handle.net/11094/23108

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