The physiological roles of vesicular GABA transporter during embryonic development : a study using knockout mice

Saito, Kenzi; Kakizaki, Toshikazu; Hayashi, Ryotaro et al.

Molecular Brain, 2010, 3(40), 1-13

アクセス数:2,565件（2025-07-13 19:53 集計）

固定URL: https://hdl.handle.net/11094/23112

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タイトル
The physiological roles of vesicular GABA transporter during embryonic development : a study using knockout mice
著者
著者名
Saito, Kenzi
NRID
1000050375531 1000050375531
著者名
Kakizaki, Toshikazu
著者名
Hayashi, Ryotaro
NRID
1000020302408 1000020302408
著者名
Nishimaru, Hiroshi
NRID
1000060402369 1000060402369
著者名
Furukawa, Tomonori
NRID
1000010106908 1000010106908
著者名
Nakazato, Yoichi
NRID
1000010397002 1000010397002
著者名
Takamori, Shigeo
著者名
Ebihara, Satoe
著者名
Uematsu, Masakazu
NRID
1000080144351 1000080144351
著者名
Mishina, Masayoshi
NRID
1000080229830 1000080229830
著者名
Miyazaki, Jun-ichi
NRID
1000040090930 1000040090930
著者名
Yokoyama, Minesuke
NRID
1000020014277 1000020014277
著者名
Konishi, Shiro
NRID
1000080345818 1000080345818
著者名
Inoue, Koichi
NRID
1000050254272 1000050254272
著者名
Fukuda, Atsuo
NRID
1000060156809 1000060156809
著者名
Fukumoto, Manabu
NRID
1000010075541 1000010075541
著者名
Nakamura, Kenji
NRID
1000060013976 1000060013976
著者名
Obata, Kunihiko
NRID
1000090202366 1000090202366
著者名
Yanagawa, Yuchio
抄録
Background: The vesicular GABA transporter (VGAT) loads GABA and glycine from the neuronal cytoplasm into synaptic vesicles. To address functional importance of VGAT during embryonic development, we generated global VGAT knockout mice and analyzed them.
Results: VGAT knockouts at embryonic day (E) 18.5 exhibited substantial increases in overall GABA and glycine, but not glutamate, contents in the forebrain. Electrophysiological recordings from E17.5-18.5 spinal cord motoneurons demonstrated that VGAT knockouts presented no spontaneous inhibitory postsynaptic currents mediated by GABA and glycine. Histological examination of E18.5 knockout fetuses revealed reductions in the trapezius muscle, hepatic congestion and little alveolar spaces in the lung, indicating that the development of skeletal muscle, liver and lung in these mice was severely affected.
Conclusion: VGAT is fundamental for the GABA- and/or glycine-mediated transmission that supports embryonic development. VGAT knockout mice will be useful for further investigating the roles of VGAT in normal physiology and pathophysiologic processes.
出版者
BioMed Central
収録物名
Molecular Brain
巻(号)
3(40)
ページ
1-13
刊行年月
2010-12-30
言語
英語
ハンドルURL
https://hdl.handle.net/11094/23112
eISSN
17566606
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1186/1756-6606-3-40
アクセス権
open access
権利情報
© 2010 Saito et al; licensee BioMed Central Ltd. This article is licensed under a Creative Commons Attribution 2.0 Generic License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article