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論文情報

タイトル
The physiological roles of vesicular GABA transporter during embryonic development : a study using knockout mice
著者
Saito, Kenzi Saito, Kenzi
Kakizaki, Toshikazu Kakizaki, Toshikazu
Hayashi, Ryotaro Hayashi, Ryotaro
Nishimaru, Hiroshi Nishimaru, Hiroshi
Furukawa, Tomonori Furukawa, Tomonori
Nakazato, Yoichi Nakazato, Yoichi
Takamori, Shigeo Takamori, Shigeo
Ebihara, Satoe Ebihara, Satoe
Uematsu, Masakazu Uematsu, Masakazu
Mishina, Masayoshi Mishina, Masayoshi
Miyazaki, Jun-ichi Miyazaki, Jun-ichi
Yokoyama, Minesuke Yokoyama, Minesuke
Konishi, Shiro Konishi, Shiro
Inoue, Koichi Inoue, Koichi
Fukuda, Atsuo Fukuda, Atsuo
Fukumoto, Manabu Fukumoto, Manabu
Nakamura, Kenji Nakamura, Kenji
Obata, Kunihiko Obata, Kunihiko
Yanagawa, Yuchio Yanagawa, Yuchio
抄録
Background: The vesicular GABA transporter (VGAT) loads GABA and glycine from the neuronal cytoplasm into synaptic vesicles. To address functional importance of VGAT during embryonic development, we generated global VGAT knockout mice and analyzed them.
Results: VGAT knockouts at embryonic day (E) 18.5 exhibited substantial increases in overall GABA and glycine, but not glutamate, contents in the forebrain. Electrophysiological recordings from E17.5-18.5 spinal cord motoneurons demonstrated that VGAT knockouts presented no spontaneous inhibitory postsynaptic currents mediated by GABA and glycine. Histological examination of E18.5 knockout fetuses revealed reductions in the trapezius muscle, hepatic congestion and little alveolar spaces in the lung, indicating that the development of skeletal muscle, liver and lung in these mice was severely affected.
Conclusion: VGAT is fundamental for the GABA- and/or glycine-mediated transmission that supports embryonic development. VGAT knockout mice will be useful for further investigating the roles of VGAT in normal physiology and pathophysiologic processes.
公開者
BioMed Central
掲載誌名
Molecular Brain
巻
3
号
40
開始ページ
1
終了ページ
13
刊行年月
2010-12-30
ISSN
17566606
DOI (出版社版)
info:doi/10.1186/1756-6606-3-40
URL
http://hdl.handle.net/11094/23112
権利情報
Copyright © 2010 Saito et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
言語
英語
カテゴリ
学術雑誌論文 Journal Article

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