An ATP-Dependent Inwardly Rectifying Potassium Channel, KAB-2(Kir4.1), in Cochlear Stria Vascularis of Inner Ear : Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential

日比野, 浩; ヒビノ, ヒロシ

doi:https://doi.org/10.11501/3155285

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タイトル	An ATP-Dependent Inwardly Rectifying Potassium Channel, KAB-2(Kir4.1), in Cochlear Stria Vascularis of Inner Ear : Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential
	内耳蝸牛血管条におけるATP依存性内向き整流カリウムチャネル, KAB-2(Kir4.1) : その特異的な分布と蝸牛内高電位の成立との相関
	ナイジ　カギュウ　ケッカンジョウ　ニ　オケル　ATP　イゾンセイ　ウチムキ　セイリュウ　カリウム　チャネル　KAB-2　Kir4.1　ソノ　トクイテキナ　ブンプ　ト　カギュウナイ　コウデンイ　ノ　セイリツ　トノ　ソウカン
著者	著者名日比野, 浩 ; ヒビノ, ヒロシ
内容	Hibino, Hiroshi; Horio, Yoshiyuki; Inanobe, Atsushi; Doi, Katsumi; Ito, Minoru; Yamada, Mitsuhiko; Gotow, Takahiro; Uchiyama, Yasuo; Kawamura, Masaru; Kubo, Takeshi; Kurachi, Yoshihisa. An ATP-Dependent Inwardly Rectifying Potassium Channel, KAB-2(Kir4.1), in Cochlear Stria Vascularis of Inner Ear : Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential. Journal of Neuroscience. 1997. 17(12), p.4711-4721.
抄録	Cochlear endolymph has a highly positive potential of approximately +80 mV. This so-called endocochlear potential (EP) is essential for hearing. Although pivotal roles of K+ channels in the formation of EP have been suggested, the types and distribution of K+ channels in cochlea have not been characterized. Because EP was depressed by vascular perfusion of Ba2+, an inhibitor of inwardly rectifying K+ (Kir) channels, but not by either 4-aminopyridine or tetraethylammonium, we examined the expression of Kir channel subunits in cochlear stria vascularis, the tissue that is supposed to play the central role in the generation of positive EP. Of 11 members of the Kir channel family examined with reverse transcription-PCR, we could detect only expression of KAB-2 (Kir4.1) mRNA in stria vascularis. KAB-2 immunoreactivity was specifically localized at the basolateral membrane of marginal cells but not in either basal or intermediate cells. Developmental expression of KAB-2 in marginal cells paralleled formation of EP. Furthermore, deaf mutant mice (viable dominant spotting; WV/WV) expressed no KAB-2 in their marginal cells. These results suggest that KAB-2 in marginal cells may be critically involved in the generation of positive EP.
学位名	博士（医学）
学位授与年月日	1999-03-25
学位授与機関	大阪大学
研究科	医学系
専攻	外科系
学位授与番号	甲第06800号
学位記番号	14531
言語	英語
ハンドルURL	https://hdl.handle.net/11094/2998
DOI	https://doi.org/10.11501/3155285
関連情報 (references)	URI http://www.jneurosci.org/cgi/content/abstract/17/12/4711
アクセス権	open access
権利情報	Copyright: Society for Neuroscience
出版タイプ	Version of Record
カテゴリ	博士論文本文あり / 医学系研究科 / 1998年度
カテゴリ	博士論文 / 医学系研究科 / 1998年度

資源タイプ	博士論文
ローカル資源タイプ	博士論文本文あり
ローカル資源タイプ	博士論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	大阪大学, 1999, 博士論文
DC.title	An ATP-Dependent Inwardly Rectifying Potassium Channel, KAB-2(Kir4.1), in Cochlear Stria Vascularis of Inner Ear : Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential
	内耳蝸牛血管条におけるATP依存性内向き整流カリウムチャネル, KAB-2(Kir4.1) : その特異的な分布と蝸牛内高電位の成立との相関
	ナイジ　カギュウ　ケッカンジョウ　ニ　オケル　ATP　イゾンセイ　ウチムキ　セイリュウ　カリウム　チャネル　KAB-2　Kir4.1　ソノ　トクイテキナ　ブンプ　ト　カギュウナイ　コウデンイ　ノ　セイリツ　トノ　ソウカン
DC.creator	日比野, 浩
DC.creator	ヒビノ, ヒロシ
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DC.relation' scheme='DCTERMS.URI	http://www.jneurosci.org/cgi/content/abstract/17/12/4711
DC.description	Hibino, Hiroshi; Horio, Yoshiyuki; Inanobe, Atsushi; Doi, Katsumi; Ito, Minoru; Yamada, Mitsuhiko; Gotow, Takahiro; Uchiyama, Yasuo; Kawamura, Masaru; Kubo, Takeshi; Kurachi, Yoshihisa. An ATP-Dependent Inwardly Rectifying Potassium Channel, KAB-2(Kir4.1), in Cochlear Stria Vascularis of Inner Ear : Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential. Journal of Neuroscience. 1997. 17(12), p.4711-4721.
DCTERMS.abstract	Cochlear endolymph has a highly positive potential of approximately +80 mV. This so-called endocochlear potential (EP) is essential for hearing. Although pivotal roles of K+ channels in the formation of EP have been suggested, the types and distribution of K+ channels in cochlea have not been characterized. Because EP was depressed by vascular perfusion of Ba2+, an inhibitor of inwardly rectifying K+ (Kir) channels, but not by either 4-aminopyridine or tetraethylammonium, we examined the expression of Kir channel subunits in cochlear stria vascularis, the tissue that is supposed to play the central role in the generation of positive EP. Of 11 members of the Kir channel family examined with reverse transcription-PCR, we could detect only expression of KAB-2 (Kir4.1) mRNA in stria vascularis. KAB-2 immunoreactivity was specifically localized at the basolateral membrane of marginal cells but not in either basal or intermediate cells. Developmental expression of KAB-2 in marginal cells paralleled formation of EP. Furthermore, deaf mutant mice (viable dominant spotting; WV/WV) expressed no KAB-2 in their marginal cells. These results suggest that KAB-2 in marginal cells may be critically involved in the generation of positive EP.
DCTERMS.issued' scheme='DCTERMS.W3CDTF	1999-03-25
DC.rights	Copyright: Society for Neuroscience
DC.identifier	10.11501/3155285
DC.format	application/pdf
DC.format	application/pdf
citation_doi	https://doi.org/10.11501/3155285
citation_title	An ATP-Dependent Inwardly Rectifying Potassium Channel, KAB-2(Kir4.1), in Cochlear Stria Vascularis of Inner Ear : Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential
	内耳蝸牛血管条におけるATP依存性内向き整流カリウムチャネル, KAB-2(Kir4.1) : その特異的な分布と蝸牛内高電位の成立との相関
	ナイジ　カギュウ　ケッカンジョウ　ニ　オケル　ATP　イゾンセイ　ウチムキ　セイリュウ　カリウム　チャネル　KAB-2　Kir4.1　ソノ　トクイテキナ　ブンプ　ト　カギュウナイ　コウデンイ　ノ　セイリツ　トノ　ソウカン
citation_author	日比野, 浩
citation_author	ヒビノ, ヒロシ
citation_language	英語
citation_public_url	https://hdl.handle.net/11094/2998
citation_abstract_html_url	http://www.jneurosci.org/cgi/content/abstract/17/12/4711
citation_date	1999-03-25
citation_doi_detail	10.11501/3155285

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