Protection from Anti-TCR/CD3-induced Apoptosis in Immature Thymocytes by a Signal through Thymic Shared Antigen-1/Stem Cell Antigen-2

野田, 敏司

doi:https://doi.org/10.11501/3128887

Protection from Anti-TCR/CD3-induced Apoptosis in Immature Thymocytes by a Signal through Thymic Shared Antigen-1/Stem Cell Antigen-2

野田, 敏司

アクセス数:488件（2025-05-09 23:15 集計）

固定URL: https://doi.org/10.11501/3128887

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タイトル	Protection from Anti-TCR/CD3-induced Apoptosis in Immature Thymocytes by a Signal through Thymic Shared Antigen-1/Stem Cell Antigen-2
	TSA-1/Sca-2分子ハT細胞受容体ヲ介シタアポトーシスヲ回避サセル機能ヲモツ
	TSA-1/Sca-2 ブンシハT サイボウジュヨウタイヲカイシタアポトーシスヲカイヒサセルキノウヲモツ
著者	NRID 1000010297260 1000010297260 著者名野田, 敏司 ; ノダ, サトシ
内容	Noda, Satoshi; Kosugi, Atsushi; Saitoh, Shin-ichiroh; Narumiya, Seiji; Hamaoka, Toshiyuki. Protection from Anti-TCR/CD3-induced Apoptosis in Immature Thymocytes by a Signal through Thymic Shared Antigen-1/Stem Cell Antigen-2. Journal of Experimental Medicine. 1996. 183(5), p.2355-2360.
抄録	During T cell development in the thymus, the expression of thymic shared antigen-1 (TSA-1)/stem cell antigen-2 (Sca-2), a glycosylphosphatidylinositol (GPI)-anchored differentiation antigen, is developmentally regulated. The expression level of TSA-1 is the highest in most immature CD4- CD8- thymocytes, high in CD4+ CD8+ thymocytes, but barely detectable in mature CD4+ CD8- or CD4- CD8- thymocytes and peripheral T cells. We have previously shown that surface TSA-1 expression in peripheral T cells is induced upon activation and that anti-TSA-1 mAb inhibits the T cell receptor (TCR) signaling pathway in activated T cells. In the present study, we have analyzed a role of TSA- 1 in thymic selection events, especially in TCR-mediated apoptosis. In in vitro experiments, anti-TSA-1 blocked anti-CD3-induced cell death of T cell hybridomas. When anti-TSA-1 was injected into newborn mice in vivo together with anti-CD3 epsilon or anti-TCR-beta, TCR/CD3-mediated apoptosis of thymocytes was almost completely blocked. The blockade of apoptosis was defined by the inhibition of, first, the decrease in total number of thymocytes; second, the decrease in percentages of CD4+ CD8+ thymocytes; and third, the induction of DNA fragmentation. However, anti-TSA-1 did not block either steroid- or radiation-induced apoptosis, indicating that a signal via TSA-1 does not inhibit a common pathway of thymocyte apoptosis. Since TCR-mediated apoptosis is pivotal in thymic ontogeny, these results suggest that TSA-1/Sca-2 is an important cell surface molecule regulating the fate of a developing T cell.
学位名	博士（医学）
学位授与年月日	1997-03-25
学位授与機関	大阪大学
研究科	医学
専攻	生理系
学位授与番号	甲第05800号
学位記番号	12986
言語	英語
ハンドルURL	https://hdl.handle.net/11094/3367
DOI	https://doi.org/10.11501/3128887
アクセス権	open access
権利情報	Copyright: Rockefeller University Press
出版タイプ	Version of Record
カテゴリ	博士論文本文あり / 医学系研究科 / 1996年度
カテゴリ	博士論文 / 医学系研究科 / 1996年度

資源タイプ	博士論文
ローカル資源タイプ	博士論文本文あり
ローカル資源タイプ	博士論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	大阪大学, 1997, 博士論文
DC.title	Protection from Anti-TCR/CD3-induced Apoptosis in Immature Thymocytes by a Signal through Thymic Shared Antigen-1/Stem Cell Antigen-2
	TSA-1/Sca-2分子ハT細胞受容体ヲ介シタアポトーシスヲ回避サセル機能ヲモツ
	TSA-1/Sca-2 ブンシハT サイボウジュヨウタイヲカイシタアポトーシスヲカイヒサセルキノウヲモツ
DC.creator	野田, 敏司
DC.creator	ノダ, サトシ
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DC.description	Noda, Satoshi; Kosugi, Atsushi; Saitoh, Shin-ichiroh; Narumiya, Seiji; Hamaoka, Toshiyuki. Protection from Anti-TCR/CD3-induced Apoptosis in Immature Thymocytes by a Signal through Thymic Shared Antigen-1/Stem Cell Antigen-2. Journal of Experimental Medicine. 1996. 183(5), p.2355-2360.
DCTERMS.abstract	During T cell development in the thymus, the expression of thymic shared antigen-1 (TSA-1)/stem cell antigen-2 (Sca-2), a glycosylphosphatidylinositol (GPI)-anchored differentiation antigen, is developmentally regulated. The expression level of TSA-1 is the highest in most immature CD4- CD8- thymocytes, high in CD4+ CD8+ thymocytes, but barely detectable in mature CD4+ CD8- or CD4- CD8- thymocytes and peripheral T cells. We have previously shown that surface TSA-1 expression in peripheral T cells is induced upon activation and that anti-TSA-1 mAb inhibits the T cell receptor (TCR) signaling pathway in activated T cells. In the present study, we have analyzed a role of TSA- 1 in thymic selection events, especially in TCR-mediated apoptosis. In in vitro experiments, anti-TSA-1 blocked anti-CD3-induced cell death of T cell hybridomas. When anti-TSA-1 was injected into newborn mice in vivo together with anti-CD3 epsilon or anti-TCR-beta, TCR/CD3-mediated apoptosis of thymocytes was almost completely blocked. The blockade of apoptosis was defined by the inhibition of, first, the decrease in total number of thymocytes; second, the decrease in percentages of CD4+ CD8+ thymocytes; and third, the induction of DNA fragmentation. However, anti-TSA-1 did not block either steroid- or radiation-induced apoptosis, indicating that a signal via TSA-1 does not inhibit a common pathway of thymocyte apoptosis. Since TCR-mediated apoptosis is pivotal in thymic ontogeny, these results suggest that TSA-1/Sca-2 is an important cell surface molecule regulating the fate of a developing T cell.
DCTERMS.issued' scheme='DCTERMS.W3CDTF	1997-03-25
DC.rights	Copyright: Rockefeller University Press
DC.identifier	10.11501/3128887
DC.format	3367
citation_title	Protection from Anti-TCR/CD3-induced Apoptosis in Immature Thymocytes by a Signal through Thymic Shared Antigen-1/Stem Cell Antigen-2
citation_author	野田, 敏司
citation_language	英語
citation_public_url	https://hdl.handle.net/11094/3367
citation_date	1997-03-25
citation_doi_detail	10.11501/3128887
citation_doi	https://doi.org/10.11501/3128887

Protection from Anti-TCR/CD3-induced Apoptosis in Immature Thymocytes by a Signal through Thymic Shared Antigen-1/Stem Cell Antigen-2

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