author

学術雑誌論文

学術雑誌論文

text

Biopolymers.91(9) P.729-P.736

Solvent-Dependent Conformation of Amylose Tris(Phenylcarbamate) as Deduced from Scattering and Viscosity Data

Wiley

英語

2009-04

http://hdl.handle.net/11094/53323

This is the peer reviewed version of the following article: Taichi Fujii1, Ken Terao, Maiko Tsuda, Shinichi Kitamura, Takashi Norisuye, “Solvent-dependent conformation of amylose tris(phenylcarbamate) as deduced from scattering and viscosity data”, Biopolymers, Volume 91, Issue 9, pages 729–736, September 2009, which has been published in final form at DOI: 10.1002/bip.21219. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

The z-average mean-square radius of gyration 〈S^2〉_z, the particle scattering function P(k), the second virial coefficient, and the intrinsic viscosity [η] have been determined for amylose tris(phenylcarbamate) (ATPC) in methyl acetate (MEA) at 25°C, in ethyl acetate (EA) at 33°C, and in 4-methyl-2-pentanone (MIBK) at 25°C by light and small-angle X-ray scattering and viscometry as functions of the weight-average molecular weight in a range from 2 × 10^4 to 3 × 10^6. The first two solvents attain the theta state, whereas the last one is a good solvent for the amylose derivative. Analysis of the 〈S^2〉_z, P(k), and [η] data based on the wormlike chain yields h (the contour length or helix pitch per repeating unit) = 0.37 ± 0.02 and λ^<−1> (the Kuhn segment length) = 15 ± 2 nm in MEA, h = 0.39 ± 0.02 and λ^<−1> = 17 ± 2 nm in EA, and h = 0.42 ± 0.02 nm and λ^<−1> = 24 ± 2 nm in MIBK. These h values, comparable with the helix pitches (0.37–0.40 nm) per residue of amylose triesters in the crystalline state, are somewhat larger than the previously determined h of 0.33 ± 0.02 nm for ATPC in 1,4-dioxane and 2-ethoxyethanol, in which intramolecular hydrogen bonds are formed between the C[DOUBLE BOND]O and NH groups of the neighbor repeating units. The slightly extended helices of ATPC in the ketone and ester solvents are most likely due to the replacement of those hydrogen bonds by intermolecular hydrogen bonds between the NH groups of the polymer and the carbonyl groups of the solvent.

Solvent-Dependent Conformation of Amylose Tris(Phenylcarbamate) as Deduced from Scattering and Viscosity Data

Wiley

英語

2009-04

Biopolymers

91

9

729

736

10970282

http://hdl.handle.net/11094/53323