Nanoscale dynamics of protein assembly networks in supersaturated solutions

Matsushita, Y.; Sekiguchi, H.; Jae Wong, C.; Nishijima, M.; Ikezaki, K.; Hamada, D.; Goto, Y.; Sasaki, Y. C.

doi:https://doi.org/10.1038/s41598-017-14022-7

Nanoscale dynamics of protein assembly networks in supersaturated solutions

Matsushita, Y.; Sekiguchi, H.; Jae Wong, C. et al.

Scientific Reports, 2017, 7, 13883

アクセス数:1,114件（2025-10-26 22:25 集計）

固定URL: https://hdl.handle.net/11094/71265

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タイトル	Nanoscale dynamics of protein assembly networks in supersaturated solutions
著者	著者名 Matsushita, Y.
	NRID 1000000401563 1000000401563 著者名 Sekiguchi, H.
	著者名 Jae Wong, C.
	著者名 Nishijima, M.
	著者名 Ikezaki, K.
	著者名 Hamada, D.
	NRID 1000040153770 1000040153770 researchmap permalink read0014440 read0014440 著者名 Goto, Y.
	NRID 1000030344401 1000030344401 著者名 Sasaki, Y. C.
内容	Y. Matsushita, H. Sekiguchi, C. Jae Wong, M. Nishijima, K. Ikezaki, D. Hamada, Y. Goto & Y. C. Sasaki "Nanoscale Dynamics of Protein Assembly Networks in Supersaturated Solutions", Scientific Reports, Volume 7, Article number: 13883, Springer Nature, 2017
抄録	Proteins in solution are conventionally considered macromolecules. Dynamic microscopic structures in supersaturated protein solutions have received increasing attention in the study of protein crystallisation and the formation of misfolded aggregates. Here, we present a method for observing rotational dynamic structures that can detect the interaction of nanoscale lysozyme protein networks via diffracted X-ray tracking (DXT). Our DXT analysis demonstrated that the rearrangement behaviours of lysozyme networks or clusters, which are driven by local density and concentration fluctuations, generate force fields on the femtonewton to attonewton (fN – aN) scale. This quantitative parameter was previously observed in our experiments on supersaturated inorganic solutions. This commonality provides a way to clarify the solution structures of a variety of supersaturated solutions as well as to control nucleation and crystallisation in supersaturated solutions.
出版者	Springer Nature
収録物名	Scientific Reports
巻(号)	7
ページ	13883
刊行年月	2017-11
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71265
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-017-14022-7
アクセス権	open access
権利情報	© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Scientific Reports. 2017, 7, p. 13883
DC.title	Nanoscale dynamics of protein assembly networks in supersaturated solutions
DC.creator	Matsushita, Y.
	Sekiguchi, H.
	Jae Wong, C.
	Nishijima, M.
	Ikezaki, K.
	Hamada, D.
	Goto, Y.
	Sasaki, Y. C.
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2017-11
DC.identifier	https://doi.org/10.1038/s41598-017-14022-7
DC.description	Y. Matsushita, H. Sekiguchi, C. Jae Wong, M. Nishijima, K. Ikezaki, D. Hamada, Y. Goto & Y. C. Sasaki "Nanoscale Dynamics of Protein Assembly Networks in Supersaturated Solutions", Scientific Reports, Volume 7, Article number: 13883, Springer Nature, 2017
DCTERMS.abstract	Proteins in solution are conventionally considered macromolecules. Dynamic microscopic structures in supersaturated protein solutions have received increasing attention in the study of protein crystallisation and the formation of misfolded aggregates. Here, we present a method for observing rotational dynamic structures that can detect the interaction of nanoscale lysozyme protein networks via diffracted X-ray tracking (DXT). Our DXT analysis demonstrated that the rearrangement behaviours of lysozyme networks or clusters, which are driven by local density and concentration fluctuations, generate force fields on the femtonewton to attonewton (fN – aN) scale. This quantitative parameter was previously observed in our experiments on supersaturated inorganic solutions. This commonality provides a way to clarify the solution structures of a variety of supersaturated solutions as well as to control nucleation and crystallisation in supersaturated solutions.
DC.rights	© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	71265
citation_title	Nanoscale dynamics of protein assembly networks in supersaturated solutions
citation_author	Matsushita, Y.
	Sekiguchi, H.
	Jae Wong, C.
	Nishijima, M.
	Ikezaki, K.
	Hamada, D.
	Goto, Y.
	Sasaki, Y. C.
citation_publisher	Springer Nature
citation_language	英語
citation_date	2017-11
citation_journal_title	Scientific Reports
citation_volume	7
citation_firstpage	13883
citation_doi	https://doi.org/10.1038/s41598-017-14022-7
citation_public_url	https://hdl.handle.net/11094/71265

Nanoscale dynamics of protein assembly networks in supersaturated solutions

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