学術雑誌論文

学術雑誌論文

text

Scientific Reports. 2016, 6, p. 30473

A small-angle X-ray scattering study of alpha-synuclein from human red blood cells

Springer Nature

英語

text

2016-07

https://doi.org/10.1038/srep30473

Katsuya Araki, Naoto Yagi, Rie Nakatani, Hiroshi Sekiguchi, Masatomo So, Hisashi Yagi, Noboru Ohta, Yoshitaka Nagai, Yuji Goto & Hideki Mochizuki "A small-angle X-ray scattering study of alpha-synuclein from human red blood cells", Scientific Reports, volume 6, Article number: 30473, Springer Nature, 2016

α-synuclein (α-syn) is the main component of Lewy bodies, which are neuropathological hallmarks of patients with Parkinson’s disease. As it has been controversial whether human α-syn from erythrocytes exists as a tetramer under physiological conditions, we tried solving this issue by the small-angle X-ray solution scattering method. Under two different conditions (high ionic strength with a Tris buffer and low ionic strength with an ammonium acetate buffer), no evidence was found for the presence of tetramer. When comparing erythrocyte and recombinant α-syn molecules, we found no significant difference of the molecular weight and the secondary structure although the buffer conditions strongly affect the radius of gyration of the protein. The results indicate that, even though a stable tetramer may not be formed, conformation of α-syn depends much on its environment, which may be the reason for its tendency to aggregate in cells.

© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.

71266

A small-angle X-ray scattering study of alpha-synuclein from human red blood cells

Springer Nature

英語

2016-07

Scientific Reports

6

30473

https://doi.org/10.1038/srep30473

https://hdl.handle.net/11094/71266