Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson’s disease patients

Araki, Katsuya; Yagi, Naoto; Ikemoto, Yuka; Yagi, Hisashi; Choong, Chi-Jing; Hayakawa, Hideki; Beck, Goichi; Sumi, Hisae; Fujimura, Harutoshi; Moriwaki, Taro; Nagai, Yoshitaka; Goto, Yuji; Mochizuki, Hideki

doi:https://doi.org/10.1038/srep17625

Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson’s disease patients

Araki, Katsuya; Yagi, Naoto; Ikemoto, Yuka et al.

Scientific Reports, 2015, 5, 17625

アクセス数:1,585件（2025-07-12 19:52 集計）

固定URL: https://hdl.handle.net/11094/71268

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タイトル	Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson’s disease patients
著者	NRID 1000050649431 1000050649431 著者名 Araki, Katsuya
	NRID 1000080133940 1000080133940 著者名 Yagi, Naoto
	著者名 Ikemoto, Yuka
	NRID 1000010432494 1000010432494 著者名 Yagi, Hisashi
	著者名 Choong, Chi-Jing
	著者名 Hayakawa, Hideki
	NRID 1000020626353 1000020626353 researchmap permalink beck-neuro beck-neuro Scopus Author ID 53867774800 53867774800 著者名 Beck, Goichi
	NRID 1000030403059 1000030403059 著者名 Sumi, Hisae
	NRID 1000020263246 1000020263246 著者名 Fujimura, Harutoshi
	NRID 1000090372143 1000090372143 著者名 Moriwaki, Taro
	NRID 1000060335354 1000060335354 著者名 Nagai, Yoshitaka
	NRID 1000040153770 1000040153770 researchmap permalink read0014440 read0014440 著者名 Goto, Yuji
	NRID 1000090230044 1000090230044 ORCID 0000-0002-0874-7542 0000-0002-0874-7542 researchmap permalink read0207787 read0207787 Scopus Author ID 7202006099 7202006099 著者名 Mochizuki, Hideki
内容	Katsuya Araki, Naoto Yagi, Yuka Ikemoto, Hisashi Yagi, Chi-Jing Choong, Hideki Hayakawa, Goichi Beck, Hisae Sumi, Harutoshi Fujimura, Taro Moriwaki, Yoshitaka Nagai, Yuji Goto & Hideki Mochizuki "Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson’s disease patients", Scientific Reports, volume 5, Article number: 17625, Springer Nature, 2015
抄録	Lewy bodies (LBs), which mainly consist of α-synuclein (α-syn), are neuropathological hallmarks of patients with Parkinson’s disease (PD). The fine structure of LBs is unknown, and LBs cannot be made artificially. Nevertheless, many studies have described fibrillisation using recombinant α-syn purified from E. coli. An extremely fundamental problem is whether the structure of LBs is the same as that of recombinant amyloid fibrils. Thus, we used synchrotron Fourier transform infrared micro-spectroscopy (FTIRM) to analyse the fine structure of LBs in the brain of PD patients. Our results showed a shift in the infrared spectrum that indicates abundance of a β-sheet-rich structure in LBs. Also, 2D infrared mapping of LBs revealed that the content of the β-sheet structure is higher in the halo than in the core, and the core contains a large amount of proteins and lipids.
出版者	Springer Nature
収録物名	Scientific Reports
巻(号)	5
ページ	17625
刊行年月	2015-12
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71268
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/srep17625
アクセス権	open access
権利情報	© 2015 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Scientific Reports. 2015, 5, p. 17625
DC.title	Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson’s disease patients
DC.creator	Araki, Katsuya
	Yagi, Naoto
	Ikemoto, Yuka
	Yagi, Hisashi
	Choong, Chi-Jing
	Hayakawa, Hideki
	Beck, Goichi
	Sumi, Hisae
	Fujimura, Harutoshi
	Moriwaki, Taro
	Nagai, Yoshitaka
	Goto, Yuji
	Mochizuki, Hideki
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2015-12
DC.identifier	https://doi.org/10.1038/srep17625
DC.description	Katsuya Araki, Naoto Yagi, Yuka Ikemoto, Hisashi Yagi, Chi-Jing Choong, Hideki Hayakawa, Goichi Beck, Hisae Sumi, Harutoshi Fujimura, Taro Moriwaki, Yoshitaka Nagai, Yuji Goto & Hideki Mochizuki "Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson’s disease patients", Scientific Reports, volume 5, Article number: 17625, Springer Nature, 2015
DCTERMS.abstract	Lewy bodies (LBs), which mainly consist of α-synuclein (α-syn), are neuropathological hallmarks of patients with Parkinson’s disease (PD). The fine structure of LBs is unknown, and LBs cannot be made artificially. Nevertheless, many studies have described fibrillisation using recombinant α-syn purified from E. coli. An extremely fundamental problem is whether the structure of LBs is the same as that of recombinant amyloid fibrils. Thus, we used synchrotron Fourier transform infrared micro-spectroscopy (FTIRM) to analyse the fine structure of LBs in the brain of PD patients. Our results showed a shift in the infrared spectrum that indicates abundance of a β-sheet-rich structure in LBs. Also, 2D infrared mapping of LBs revealed that the content of the β-sheet structure is higher in the halo than in the core, and the core contains a large amount of proteins and lipids.
DC.rights	© 2015 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	71268
citation_title	Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson’s disease patients
citation_author	Araki, Katsuya
	Yagi, Naoto
	Ikemoto, Yuka
	Yagi, Hisashi
	Choong, Chi-Jing
	Hayakawa, Hideki
	Beck, Goichi
	Sumi, Hisae
	Fujimura, Harutoshi
	Moriwaki, Taro
	Nagai, Yoshitaka
	Goto, Yuji
	Mochizuki, Hideki
citation_publisher	Springer Nature
citation_language	英語
citation_date	2015-12
citation_journal_title	Scientific Reports
citation_volume	5
citation_firstpage	17625
citation_doi	https://doi.org/10.1038/srep17625
citation_public_url	https://hdl.handle.net/11094/71268

Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson’s disease patients

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