SOX7 and SOX17 Regulate the Parietal Endoderm-specific Enhancer Activity of Mouse Laminin α1 Gene

Niimi, Tomoaki; Hayashi, Yoshitaka; Futaki, Sugiko; Sekiguchi, Kiyotoshi

doi:info:doi/10.1074/jbc.M403724200

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タイトル	SOX7 and SOX17 Regulate the Parietal Endoderm-specific Enhancer Activity of Mouse Laminin α1 Gene
著者	Niimi, Tomoaki Niimi, Tomoaki
	Hayashi, Yoshitaka Hayashi, Yoshitaka
	Futaki, Sugiko Futaki, Sugiko
	Sekiguchi, Kiyotoshi Sekiguchi, Kiyotoshi
内容	This research was originally published in the Journal of Biological Chemistry. Tomoaki Niimi, Yoshitaka Hayashi, Sugiko Futaki and Kiyotoshi Sekiguchi. SOX7 and SOX17 Regulate the Parietal Endoderm-specific Enhancer Activity of Mouse Laminin α1 Gene. J. Biol. Chem. 2004; 279: 38055-38061 © the American Society for Biochemistry and Molecular Biology
抄録	Laminin-1 is the major component of embryonic basement membrane and consists of α1, β1, and γ1 chains. The expression of laminin-1 is induced in mouse F9 embryonal carcinoma cells upon differentiation into parietal endoderm cells. We recently identified a parietal endoderm-specific enhancer in the mouse laminin α1 (Lama1) gene and showed that Sp1/Sp3 and YY1 transcription factors were involved in the enhancer activity. Although here we identified that NF-Y binds to the enhancer sequence between Sp1/Sp3- and YY1-binding sites, all these transcription factors are ubiquitously expressed and thus are not sufficient to explain parietal endoderm-specific enhancer activity. In the present study, we further showed that SOX7 and SOX17 are involved in the regulation of parietal endoderm-specific enhancer activity of the mouse Lama1 gene. Northern blot analysis revealed that the steady-state levels of mouse Sox7 and Sox17 mRNAs increased in parallel with that of Lama1 mRNA during the differentiation of F9 cells. Both SOX7 and SOX17 markedly trans-activated the transcription of the Lama1 enhancer-reporter construct in undifferentiated F9 cells in a manner dependent on high mobility group box-mediated DNA binding. Electrophoretic mobility shift assays and mutational analyses revealed that SOX7 and SOX17 bound specifically to two SOX-binding sites within the Lama1 enhancer, and that these SOX-binding sites functioned synergistically to confer the trans-activation by SOX7 and SOX17. Furthermore, this trans-activation was dependent on the integrity of the binding sites for Sp1/Sp3 and NF-Y located at upstream of the two SOX-binding sites. These results indicate that the transcription of the mouse Lama1 gene during the differentiation of F9 cells is controlled by a combination of the actions of the ubiquitous factors, Sp1/Sp3 and NF-Y, and the parietal endoderm-specific factors, SOX7 and SOX17.
公開者	American Society for Biochemistry and Molecular Biology
掲載誌名	Journal of Biological Chemistry
巻	279
号	36
開始ページ	38055
終了ページ	38061
刊行年月	2004-09
ISSN	1083351X
NCID	AA1202441X
DOI	info:doi/10.1074/jbc.M403724200
URL	http://hdl.handle.net/11094/71429
言語	英語
カテゴリ	学術雑誌論文 Journal Article

著者版フラグ	publisher
NII資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
dcmi資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry.279(36) P.38055-P.38061
DC.title	SOX7 and SOX17 Regulate the Parietal Endoderm-specific Enhancer Activity of Mouse Laminin α1 Gene
DC.creator	Niimi, Tomoaki
	Hayashi, Yoshitaka
	Futaki, Sugiko
	Sekiguchi, Kiyotoshi
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language" scheme="DCTERMS.RFC1766	英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF	2004-09
DC.identifier	info:doi/10.1074/jbc.M403724200
DC.identifier" scheme="DCTERMS.URI	http://hdl.handle.net/11094/71429
DC.description	This research was originally published in the Journal of Biological Chemistry. Tomoaki Niimi, Yoshitaka Hayashi, Sugiko Futaki and Kiyotoshi Sekiguchi. SOX7 and SOX17 Regulate the Parietal Endoderm-specific Enhancer Activity of Mouse Laminin α1 Gene. J. Biol. Chem. 2004; 279: 38055-38061 © the American Society for Biochemistry and Molecular Biology
DCTERMS.abstract	Laminin-1 is the major component of embryonic basement membrane and consists of α1, β1, and γ1 chains. The expression of laminin-1 is induced in mouse F9 embryonal carcinoma cells upon differentiation into parietal endoderm cells. We recently identified a parietal endoderm-specific enhancer in the mouse laminin α1 (Lama1) gene and showed that Sp1/Sp3 and YY1 transcription factors were involved in the enhancer activity. Although here we identified that NF-Y binds to the enhancer sequence between Sp1/Sp3- and YY1-binding sites, all these transcription factors are ubiquitously expressed and thus are not sufficient to explain parietal endoderm-specific enhancer activity. In the present study, we further showed that SOX7 and SOX17 are involved in the regulation of parietal endoderm-specific enhancer activity of the mouse Lama1 gene. Northern blot analysis revealed that the steady-state levels of mouse Sox7 and Sox17 mRNAs increased in parallel with that of Lama1 mRNA during the differentiation of F9 cells. Both SOX7 and SOX17 markedly trans-activated the transcription of the Lama1 enhancer-reporter construct in undifferentiated F9 cells in a manner dependent on high mobility group box-mediated DNA binding. Electrophoretic mobility shift assays and mutational analyses revealed that SOX7 and SOX17 bound specifically to two SOX-binding sites within the Lama1 enhancer, and that these SOX-binding sites functioned synergistically to confer the trans-activation by SOX7 and SOX17. Furthermore, this trans-activation was dependent on the integrity of the binding sites for Sp1/Sp3 and NF-Y located at upstream of the two SOX-binding sites. These results indicate that the transcription of the mouse Lama1 gene during the differentiation of F9 cells is controlled by a combination of the actions of the ubiquitous factors, Sp1/Sp3 and NF-Y, and the parietal endoderm-specific factors, SOX7 and SOX17.
citation_title	SOX7 and SOX17 Regulate the Parietal Endoderm-specific Enhancer Activity of Mouse Laminin α1 Gene
citation_author	Niimi, Tomoaki
	Hayashi, Yoshitaka
	Futaki, Sugiko
	Sekiguchi, Kiyotoshi
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	英語
citation_date	2004-09
citation_journal_title	Journal of Biological Chemistry
citation_volume	279
citation_issue	36
citation_firstpage	38055
citation_lastpage	38061
citation_issn	1083351X
citation_doi	info:doi/10.1074/jbc.M403724200
citation_public_url	http://hdl.handle.net/11094/71429

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