Laminin-10/11 and Fibronectin Differentially Regulate Integrin- dependent Rho and Rac Activation via p130Cas-CrkII-DOCK180 Pathway

Gu, Jianguo; Sumida, Yasuhiro; Sanzen, Noriko et al.

Journal of Biological Chemistry, 2001, 276(29), 27090-27097

アクセス数:558件（2025-07-02 15:47 集計）

固定URL: https://hdl.handle.net/11094/71436

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タイトル
Laminin-10/11 and Fibronectin Differentially Regulate Integrin- dependent Rho and Rac Activation via p130Cas-CrkII-DOCK180 Pathway
著者
NRID
1000040260369 1000040260369
著者名
Gu, Jianguo
著者名
Sumida, Yasuhiro
著者名
Sanzen, Noriko
NRID
1000050187845 1000050187845
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Scopus Author ID
7201662709 7201662709
ORCID
0000-0002-6433-1410 0000-0002-6433-1410
著者名
Sekiguchi, Kiyotoshi
内容
This research was originally published in the Journal of Biological Chemistry. Jianguo Gu, Yasuhiro Sumida, Noriko Sanzen and Kiyotoshi Sekiguchi. Laminin-10/11 and Fibronectin Differentially Regulate Integrin- dependent Rho and Rac Activation via p130Cas-CrkII-DOCK180 Pathway. J. Biol. Chem. 2001; 276: 27090–27097 © the American Society for Biochemistry and Molecular Biology
抄録
The α5 chain-containing laminin isoforms, laminins-10 and -11 (laminin-10/11), are the major components of the basement membrane, having potent cell-adhesive activity. We examined the cell-adhesive and integrin-mediated signaling activities of laminin-10/11 in comparison to fibronectin, the best characterized extracellular adhesive ligand. We found that laminin-10/11 are more active than fibronectin in promoting cell migration and preferentially activate Rac, not Rho, via the p130Cas-CrkII-DOCK180 pathway. Cells adhering to fibronectin develop stress fibers and focal contacts, whereas cells adhering to laminin-10/11 do not, consistent with the high cell migration-promoting activity of laminin-10/11. Pull-down assays of GTP-loaded Rac and Rho demonstrated the preferential activation of Rac on laminin-10/11, in contrast to the activation of Rho on fibronectin. Activation of Rac by laminin-10/11 was associated with the phosphorylation of p130Cas and an increased formation of a p130Cas-CrkII-DOCK180 complex. Cell migration on laminin-10/11 was suppressed by the expression of either a dominant-negative Rac or CrkII mutants defective in p130Casor DOCK180 binding. This is the first report demonstrating a distinct activation of Rho family GTPases resulting from adhesion to different extracellular ligands.
出版者
American Society for Biochemistry and Molecular Biology
収録物名
Journal of Biological Chemistry
巻(号)
276(29)
ページ
27090-27097
刊行年月
2001-07
言語
英語
ハンドルURL
https://hdl.handle.net/11094/71436
eISSN
1083351X
NCID
AA1202441X
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1074/jbc.M102284200
アクセス権
open access
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article