学術雑誌論文

学術雑誌論文

text

Journal of Biological Chemistry. 1983, 258(19), p. 11819-11822

A monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue in gangliosides and glycoproteins

American Society for Biochemistry and Molecular Biology

英語

text

1983-10

http://www.jbc.org/content/258/19/11819

This research was originally published in the Journal of Biological Chemistry. S Hakomori, C M Patterson, E Nudelman and K Sekiguchi. A monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue in gangliosides and glycoproteins. J. Biol. Chem. 1983; 258: 11819-11822 © the American Society for Biochemistry and Molecular Biology

A hybridoma cell line producing a monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue has been established. The antibody is IgG2b and reacts only with lacto-series gangliosides as well as with glycoproteins having an N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue, but does not react with gangliosides or glycoproteins having an N-acetylneuraminosyl-alpha 2 leads to 3- or -alpha 2 leads to 4-galactosyl residue. The antibody is useful for detecting the specific carbohydrate chain having this terminal structure by immunostaining of glycolipids separated on thin layer chromatography or glycoproteins separated on gel electrophoresis after blotting on nitrocellulose sheet. A remarkable accumulation of a few gangliosides having this terminal structure has been detected by this monoclonal antibody in some human cancer.

71447

A monoclonal antibody directed to N-acetylneuraminosyl-alpha 2 leads to 6-galactosyl residue in gangliosides and glycoproteins

American Society for Biochemistry and Molecular Biology

英語

1983-10

Journal of Biological Chemistry

258

19

11819

11822

https://hdl.handle.net/11094/71447

http://www.jbc.org/content/258/19/11819