Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining

Kurata, Ryuichiro; Futaki, Sugiko; Nakano, Itsuko; Fujita, Fumitaka; Tanemura, Atsushi; Murota, Hiroyuki; Katayama, Ichiro; Okada, Fumihiro; Sekiguchi, Kiyotoshi

doi:https://doi.org/10.1371/journal.pone.0178709

アクセス数:309件（2024-04-20 23:46 集計）

固定URL: https://hdl.handle.net/11094/71797

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タイトル	Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining
著者	著者名 Kurata, Ryuichiro
	NRID 1000000403014 1000000403014 著者名 Futaki, Sugiko
	著者名 Nakano, Itsuko
	著者名 Fujita, Fumitaka
	NRID 1000050457016 1000050457016 researchmap permalink tane1225 tane1225 Scopus Author ID 6508011282 6508011282 著者名 Tanemura, Atsushi
	NRID 1000090363499 1000090363499 著者名 Murota, Hiroyuki
	NRID 1000080191980 1000080191980 著者名 Katayama, Ichiro
	著者名 Okada, Fumihiro
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 著者名 Sekiguchi, Kiyotoshi
内容	Kurata R, Futaki S, Nakano I, Fujita F, Tanemura A, Murota H, et al. (2017) Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining. PLoS ONE 12(6): e0178709. https://doi.org/10.1371/journal.pone.0178709
抄録	Because sweat secretion is facilitated by mechanical contraction of sweat gland structures, understanding their structure-function relationship could lead to more effective treatments for patients with sweat gland disorders such as heat stroke. Conventional histological studies have shown that sweat glands are three-dimensionally coiled tubular structures consisting of ducts and secretory portions, although their detailed structural anatomy remains unclear. To better understand the details of the three-dimensional (3D) coiled structures of sweat glands, a whole-mount staining method was employed to visualize 3D coiled gland structures with sweat gland markers for ductal luminal, ductal basal, secretory luminal, and myoepithelial cells. Imaging the 3D coiled gland structures demonstrated that the ducts and secretory portions were comprised of distinct tubular structures. Ductal tubules were occasionally bent, while secretory tubules were frequently bent and formed a self-entangled coiled structure. Whole-mount staining of complex coiled gland structures also revealed the detailed 3D cellular arrangements in the individual sweat gland compartments. Ducts were composed of regularly arranged cuboidal shaped cells, while secretory portions were surrounded by myoepithelial cells longitudinally elongated along entangled secretory tubules. Whole-mount staining was also used to visualize the spatial arrangement of blood vessels and nerve fibers, both of which facilitate sweat secretion. The blood vessels ran longitudinally parallel to the sweat gland tubules, while nerve fibers wrapped around secretory tubules, but not ductal tubules. Taken together, whole-mount staining of sweat glands revealed the 3D cell shapes and arrangements of complex coiled gland structures and provides insights into the mechanical contraction of coiled gland structures during sweat secretion.
出版者	PLOS
収録物名	PLOS ONE
巻(号)	12(6)
ページ	e0178709
刊行年月	2017-06
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71797
eISSN	19326203
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1371/journal.pone.0178709
アクセス権	open access
権利情報	© 2017 Kurata et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	PLOS ONE. 2017, 12(6), p. e0178709
DC.title	Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining
DC.creator	Kurata, Ryuichiro
	Futaki, Sugiko
	Nakano, Itsuko
	Fujita, Fumitaka
	Tanemura, Atsushi
	Murota, Hiroyuki
	Katayama, Ichiro
	Okada, Fumihiro
	Sekiguchi, Kiyotoshi
DC.publisher	PLOS
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2017-06
DC.identifier	https://doi.org/10.1371/journal.pone.0178709
DC.description	Kurata R, Futaki S, Nakano I, Fujita F, Tanemura A, Murota H, et al. (2017) Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining. PLoS ONE 12(6): e0178709. https://doi.org/10.1371/journal.pone.0178709
DCTERMS.abstract	Because sweat secretion is facilitated by mechanical contraction of sweat gland structures, understanding their structure-function relationship could lead to more effective treatments for patients with sweat gland disorders such as heat stroke. Conventional histological studies have shown that sweat glands are three-dimensionally coiled tubular structures consisting of ducts and secretory portions, although their detailed structural anatomy remains unclear. To better understand the details of the three-dimensional (3D) coiled structures of sweat glands, a whole-mount staining method was employed to visualize 3D coiled gland structures with sweat gland markers for ductal luminal, ductal basal, secretory luminal, and myoepithelial cells. Imaging the 3D coiled gland structures demonstrated that the ducts and secretory portions were comprised of distinct tubular structures. Ductal tubules were occasionally bent, while secretory tubules were frequently bent and formed a self-entangled coiled structure. Whole-mount staining of complex coiled gland structures also revealed the detailed 3D cellular arrangements in the individual sweat gland compartments. Ducts were composed of regularly arranged cuboidal shaped cells, while secretory portions were surrounded by myoepithelial cells longitudinally elongated along entangled secretory tubules. Whole-mount staining was also used to visualize the spatial arrangement of blood vessels and nerve fibers, both of which facilitate sweat secretion. The blood vessels ran longitudinally parallel to the sweat gland tubules, while nerve fibers wrapped around secretory tubules, but not ductal tubules. Taken together, whole-mount staining of sweat glands revealed the 3D cell shapes and arrangements of complex coiled gland structures and provides insights into the mechanical contraction of coiled gland structures during sweat secretion.
DC.rights	© 2017 Kurata et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	application/pdf
citation_title	Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining
citation_author	Kurata, Ryuichiro
	Futaki, Sugiko
	Nakano, Itsuko
	Fujita, Fumitaka
	Tanemura, Atsushi
	Murota, Hiroyuki
	Katayama, Ichiro
	Okada, Fumihiro
	Sekiguchi, Kiyotoshi
citation_publisher	PLOS
citation_language	英語
citation_date	2017-06
citation_journal_title	PLOS ONE
citation_volume	12
citation_issue	6
citation_firstpage	e0178709
citation_doi	https://doi.org/10.1371/journal.pone.0178709
citation_public_url	https://hdl.handle.net/11094/71797

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