Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining

Kurata, Ryuichiro; Futaki, Sugiko; Nakano, Itsuko; Fujita, Fumitaka; Tanemura, Atsushi; Murota, Hiroyuki; Katayama, Ichiro; Okada, Fumihiro; Sekiguchi, Kiyotoshi

doi:info:doi/10.1371/journal.pone.0178709

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タイトル	Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining
著者	Kurata, Ryuichiro Kurata, Ryuichiro
	Futaki, Sugiko Futaki, Sugiko
	Nakano, Itsuko Nakano, Itsuko
	Fujita, Fumitaka Fujita, Fumitaka
	Tanemura, Atsushi Tanemura, Atsushi
	Murota, Hiroyuki Murota, Hiroyuki
	Katayama, Ichiro Katayama, Ichiro
	Okada, Fumihiro Okada, Fumihiro
	Sekiguchi, Kiyotoshi Sekiguchi, Kiyotoshi
内容	Kurata R, Futaki S, Nakano I, Fujita F, Tanemura A, Murota H, et al. (2017) Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining. PLoS ONE 12(6): e0178709. https://doi.org/10.1371/journal.pone.0178709
抄録	Because sweat secretion is facilitated by mechanical contraction of sweat gland structures, understanding their structure-function relationship could lead to more effective treatments for patients with sweat gland disorders such as heat stroke. Conventional histological studies have shown that sweat glands are three-dimensionally coiled tubular structures consisting of ducts and secretory portions, although their detailed structural anatomy remains unclear. To better understand the details of the three-dimensional (3D) coiled structures of sweat glands, a whole-mount staining method was employed to visualize 3D coiled gland structures with sweat gland markers for ductal luminal, ductal basal, secretory luminal, and myoepithelial cells. Imaging the 3D coiled gland structures demonstrated that the ducts and secretory portions were comprised of distinct tubular structures. Ductal tubules were occasionally bent, while secretory tubules were frequently bent and formed a self-entangled coiled structure. Whole-mount staining of complex coiled gland structures also revealed the detailed 3D cellular arrangements in the individual sweat gland compartments. Ducts were composed of regularly arranged cuboidal shaped cells, while secretory portions were surrounded by myoepithelial cells longitudinally elongated along entangled secretory tubules. Whole-mount staining was also used to visualize the spatial arrangement of blood vessels and nerve fibers, both of which facilitate sweat secretion. The blood vessels ran longitudinally parallel to the sweat gland tubules, while nerve fibers wrapped around secretory tubules, but not ductal tubules. Taken together, whole-mount staining of sweat glands revealed the 3D cell shapes and arrangements of complex coiled gland structures and provides insights into the mechanical contraction of coiled gland structures during sweat secretion.
公開者	PLOS
掲載誌名	PLOS ONE
巻	12
号	6
開始ページ	e0178709
刊行年月	2017-06
ISSN	19326203
DOI	info:doi/10.1371/journal.pone.0178709
URL	http://hdl.handle.net/11094/71797
権利情報	© 2017 Kurata et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
言語	英語
カテゴリ	学術雑誌論文 Journal Article

著者版フラグ	publisher
NII資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
dcmi資源タイプ	text
DCTERMS.bibliographicCitation	PLOS ONE.12(6) P.e0178709
DC.title	Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining
DC.creator	Kurata, Ryuichiro
	Futaki, Sugiko
	Nakano, Itsuko
	Fujita, Fumitaka
	Tanemura, Atsushi
	Murota, Hiroyuki
	Katayama, Ichiro
	Okada, Fumihiro
	Sekiguchi, Kiyotoshi
DC.publisher	PLOS
DC.language" scheme="DCTERMS.RFC1766	英語
DCTERMS.issued" scheme="DCTERMS.W3CDTF	2017-06
DC.identifier	info:doi/10.1371/journal.pone.0178709
DC.identifier" scheme="DCTERMS.URI	http://hdl.handle.net/11094/71797
DC.description	Kurata R, Futaki S, Nakano I, Fujita F, Tanemura A, Murota H, et al. (2017) Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining. PLoS ONE 12(6): e0178709. https://doi.org/10.1371/journal.pone.0178709
DCTERMS.abstract	Because sweat secretion is facilitated by mechanical contraction of sweat gland structures, understanding their structure-function relationship could lead to more effective treatments for patients with sweat gland disorders such as heat stroke. Conventional histological studies have shown that sweat glands are three-dimensionally coiled tubular structures consisting of ducts and secretory portions, although their detailed structural anatomy remains unclear. To better understand the details of the three-dimensional (3D) coiled structures of sweat glands, a whole-mount staining method was employed to visualize 3D coiled gland structures with sweat gland markers for ductal luminal, ductal basal, secretory luminal, and myoepithelial cells. Imaging the 3D coiled gland structures demonstrated that the ducts and secretory portions were comprised of distinct tubular structures. Ductal tubules were occasionally bent, while secretory tubules were frequently bent and formed a self-entangled coiled structure. Whole-mount staining of complex coiled gland structures also revealed the detailed 3D cellular arrangements in the individual sweat gland compartments. Ducts were composed of regularly arranged cuboidal shaped cells, while secretory portions were surrounded by myoepithelial cells longitudinally elongated along entangled secretory tubules. Whole-mount staining was also used to visualize the spatial arrangement of blood vessels and nerve fibers, both of which facilitate sweat secretion. The blood vessels ran longitudinally parallel to the sweat gland tubules, while nerve fibers wrapped around secretory tubules, but not ductal tubules. Taken together, whole-mount staining of sweat glands revealed the 3D cell shapes and arrangements of complex coiled gland structures and provides insights into the mechanical contraction of coiled gland structures during sweat secretion.
DC.rights	© 2017 Kurata et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
citation_title	Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining
citation_author	Kurata, Ryuichiro
	Futaki, Sugiko
	Nakano, Itsuko
	Fujita, Fumitaka
	Tanemura, Atsushi
	Murota, Hiroyuki
	Katayama, Ichiro
	Okada, Fumihiro
	Sekiguchi, Kiyotoshi
citation_publisher	PLOS
citation_language	英語
citation_date	2017-06
citation_journal_title	PLOS ONE
citation_volume	12
citation_issue	6
citation_firstpage	e0178709
citation_issn	19326203
citation_doi	info:doi/10.1371/journal.pone.0178709
citation_public_url	http://hdl.handle.net/11094/71797

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