Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells

Ohta, Ryo; Niwa, Akira; Taniguchi, Yukimasa; Suzuki, Naoya M.; Toga, Junko; Yagi, Emiko; Saiki, Norikazu; Nishinaka, Yoko; Okada, Chihiro; Watanabe, Akira; Nakahata, Tatsutoshi; Sekiguchi, Kiyotoshi; Saito, Megumu K.

doi:https://doi.org/10.1038/srep35680

Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells

Ohta, Ryo; Niwa, Akira; Taniguchi, Yukimasa et al.

Scientific Reports, 2016, 6, 35680

アクセス数:407件（2025-03-19 01:18 集計）

固定URL: https://hdl.handle.net/11094/71803

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タイトル	Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells
著者	著者名 Ohta, Ryo
	NRID 1000020546999 1000020546999 著者名 Niwa, Akira
	著者名 Taniguchi, Yukimasa
	著者名 Suzuki, Naoya M.
	著者名 Toga, Junko
	著者名 Yagi, Emiko
	著者名 Saiki, Norikazu
	著者名 Nishinaka, Yoko 著者の別表記 Arai, Yoko
	著者名 Okada, Chihiro
	NRID 1000060506765 1000060506765 著者名 Watanabe, Akira
	NRID 1000020110744 1000020110744 著者名 Nakahata, Tatsutoshi
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 著者名 Sekiguchi, Kiyotoshi
	NRID 1000090535486 1000090535486 著者名 Saito, Megumu K.
内容	Ohta, R. et al. Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Sci. Rep. 6, 35680; doi: 10.1038/srep35680 (2016)
抄録	Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem cells (hPSCs) is an essential step for their clinical application. Among the various conditions that should be optimized, the precise role and contribution of the extracellular matrix (ECM) during differentiation are relatively unclear. Here, using a short fragment of laminin 411 (LM411-E8), an ECM predominantly expressed in the vascular endothelial basement membrane, we demonstrate that the directed switching of defined ECMs robustly yields highly-purified (>95%) endothelial progenitor cells (PSC-EPCs) without cell sorting from hPSCs in an integrin-laminin axis-dependent manner. Single-cell RNA-seq analysis revealed that LM411-E8 resolved intercellular transcriptional heterogeneity and escorted the progenitor cells to the appropriate differentiation pathway. The PSC-EPCs gave rise to functional endothelial cells both in vivo and in vitro. We therefore propose that sequential switching of defined matrices is an important concept for guiding cells towards desired fate.
出版者	Springer Nature
収録物名	Scientific Reports
巻(号)	6
ページ	35680
刊行年月	2016-11
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71803
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/srep35680
アクセス権	open access
権利情報	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Scientific Reports. 2016, 6, p. 35680
DC.title	Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells
DC.creator	Ohta, Ryo
	Niwa, Akira
	Taniguchi, Yukimasa
	Suzuki, Naoya M.
	Toga, Junko
	Yagi, Emiko
	Saiki, Norikazu
	Nishinaka, Yoko
	Okada, Chihiro
	Watanabe, Akira
	Nakahata, Tatsutoshi
	Sekiguchi, Kiyotoshi
	Saito, Megumu K.
DC.creator	Arai, Yoko
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2016-11
DC.identifier	https://doi.org/10.1038/srep35680
DC.description	Ohta, R. et al. Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells. Sci. Rep. 6, 35680; doi: 10.1038/srep35680 (2016)
DCTERMS.abstract	Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem cells (hPSCs) is an essential step for their clinical application. Among the various conditions that should be optimized, the precise role and contribution of the extracellular matrix (ECM) during differentiation are relatively unclear. Here, using a short fragment of laminin 411 (LM411-E8), an ECM predominantly expressed in the vascular endothelial basement membrane, we demonstrate that the directed switching of defined ECMs robustly yields highly-purified (>95%) endothelial progenitor cells (PSC-EPCs) without cell sorting from hPSCs in an integrin-laminin axis-dependent manner. Single-cell RNA-seq analysis revealed that LM411-E8 resolved intercellular transcriptional heterogeneity and escorted the progenitor cells to the appropriate differentiation pathway. The PSC-EPCs gave rise to functional endothelial cells both in vivo and in vitro. We therefore propose that sequential switching of defined matrices is an important concept for guiding cells towards desired fate.
DC.rights	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	71803
citation_title	Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells
citation_author	Ohta, Ryo
	Niwa, Akira
	Taniguchi, Yukimasa
	Suzuki, Naoya M.
	Toga, Junko
	Yagi, Emiko
	Saiki, Norikazu
	Nishinaka, Yoko
	Okada, Chihiro
	Watanabe, Akira
	Nakahata, Tatsutoshi
	Sekiguchi, Kiyotoshi
	Saito, Megumu K.
citation_publisher	Springer Nature
citation_language	英語
citation_date	2016-11
citation_journal_title	Scientific Reports
citation_volume	6
citation_firstpage	35680
citation_doi	https://doi.org/10.1038/srep35680
citation_public_url	https://hdl.handle.net/11094/71803

Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells

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