Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells

Miyazaki, Takamichi; Futaki, Sugiko; Suemori, Hirofumi; Taniguchi, Yukimasa; Yamada, Masashi; Kawasaki, Miwa; Hayashi, Maria; Kumagai, Hideaki; Nakatsuji, Norio; Sekiguchi, Kiyotoshi; Kawase, Eihachiro

doi:https://doi.org/10.1038/ncomms2231

Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells

Miyazaki, Takamichi; Futaki, Sugiko; Suemori, Hirofumi et al.

Nature Communications, 2012, 3, 1236

アクセス数:1,216件（2025-12-05 16:44 集計）

固定URL: https://hdl.handle.net/11094/71806

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タイトル	Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells
著者	著者名 Miyazaki, Takamichi
	NRID 1000000403014 1000000403014 著者名 Futaki, Sugiko
	NRID 1000090261198 1000090261198 著者名 Suemori, Hirofumi
	著者名 Taniguchi, Yukimasa
	NRID 1000090304055 1000090304055 著者名 Yamada, Masashi
	著者名 Kawasaki, Miwa
	著者名 Hayashi, Maria
	著者名 Kumagai, Hideaki
	NRID 1000080237312 1000080237312 著者名 Nakatsuji, Norio
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 著者名 Sekiguchi, Kiyotoshi
	NRID 1000070402790 1000070402790 著者名 Kawase, Eihachiro
内容	Miyazaki, T. et al.. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3:1236 doi: 10.1038/ncomms2231 (2012).
抄録	Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications.
出版者	Springer Nature
収録物名	Nature Communications
巻(号)	3
ページ	1236
刊行年月	2012-12
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71806
eISSN	20411723
NCID	AA12645905
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/ncomms2231
アクセス権	open access
権利情報	© 2012 The Author(s). This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Nature Communications. 2012, 3, p. 1236
DC.title	Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells
DC.creator	Miyazaki, Takamichi
	Futaki, Sugiko
	Suemori, Hirofumi
	Taniguchi, Yukimasa
	Yamada, Masashi
	Kawasaki, Miwa
	Hayashi, Maria
	Kumagai, Hideaki
	Nakatsuji, Norio
	Sekiguchi, Kiyotoshi
	Kawase, Eihachiro
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2012-12
DC.identifier	https://doi.org/10.1038/ncomms2231
DC.description	Miyazaki, T. et al.. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat. Commun. 3:1236 doi: 10.1038/ncomms2231 (2012).
DCTERMS.abstract	Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications.
DC.rights	© 2012 The Author(s). This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.
DC.format	71806
citation_title	Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells
citation_author	Miyazaki, Takamichi
	Futaki, Sugiko
	Suemori, Hirofumi
	Taniguchi, Yukimasa
	Yamada, Masashi
	Kawasaki, Miwa
	Hayashi, Maria
	Kumagai, Hideaki
	Nakatsuji, Norio
	Sekiguchi, Kiyotoshi
	Kawase, Eihachiro
citation_publisher	Springer Nature
citation_language	英語
citation_date	2012-12
citation_journal_title	Nature Communications
citation_volume	3
citation_firstpage	1236
citation_doi	https://doi.org/10.1038/ncomms2231
citation_public_url	https://hdl.handle.net/11094/71806

Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells

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