Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche

Sato, Yuya; Kiyozumi, Daiji; Futaki, Sugiko; Nakano, Itsuko; Shimono, Chisei; Kaneko, Naoko; Ikawa, Masahito; Okabe, Masaru; Sawamoto, Kazunobu; Sekiguchi, Kiyotoshi

doi:https://doi.org/10.1091/mbc.E18-05-0286

Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche

Sato, Yuya; Kiyozumi, Daiji; Futaki, Sugiko et al.

Molecular Biology of the Cell, 2018, 30(1), 56-68

アクセス数:370件（2025-04-18 02:52 集計）

固定URL: https://hdl.handle.net/11094/71809

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タイトル	Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche
著者	著者名 Sato, Yuya
	NRID 1000070452430 1000070452430 ORCID 0000-0003-3289-227X 0000-0003-3289-227X researchmap permalink dkiyozumi dkiyozumi Scopus Author ID 6506341053 6506341053 著者名 Kiyozumi, Daiji
	NRID 1000000403014 1000000403014 著者名 Futaki, Sugiko
	著者名 Nakano, Itsuko
	著者名 Shimono, Chisei
	NRID 1000020464571 1000020464571 著者名 Kaneko, Naoko
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000030089875 1000030089875 著者名 Okabe, Masaru
	NRID 1000090282350 1000090282350 著者名 Sawamoto, Kazunobu
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 著者名 Sekiguchi, Kiyotoshi
内容	Yuya Sato, Daiji Kiyozumi, Sugiko Futaki, Itsuko Nakano, Chisei Shimono, Naoko Kaneko, Masahito Ikawa, Masaru Okabe, Kazunobu Sawamoto, and Kiyotoshi Sekiguchi, "Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche", Molecular Biology of the Cell, Vol. 30, No.1, 56-68, American Society for Cell Biology, 2018
抄録	Neural stem cells (NSCs) are retained in the adult ventricular–subventricular zone (V-SVZ), a specialized neurogenic niche with a unique cellular architecture. It currently remains unclear whether or how NSCs utilize basement membranes (BMs) in this niche. Here, we examine the molecular compositions and functions of BMs in the adult mouse V-SVZ. Whole-mount V-SVZ immunostaining revealed that fractones, which are fingerlike processes of extravascular BMs, are speckled BMs unconnected to the vasculature, and differ in their molecular composition from vascular BMs. Glial fibrillary acidic protein (GFAP)-positive astrocytes and NSCs produce and adhere to speckled BMs. Furthermore, Gfap-Cre-mediated Lamc1flox(E1605Q) knockin mice, in which integrin-binding activities of laminins are specifically nullified in GFAP-positive cells, exhibit a decreased number and size of speckled BMs and reduced in vitro neurosphere-forming activity. Our results reveal niche activities of fractones/speckled BMs for NSCs and provide molecular insights into how laminin–integrin interactions regulate NSCs in vivo.
出版者	American Society for Cell Biology
収録物名	Molecular Biology of the Cell
巻(号)	30(1)
ページ	56-68
刊行年月	2018-10
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71809
eISSN	19394586
NCID	AA12024500
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1091/mbc.E18-05-0286
アクセス権	open access
権利情報	© 2019 Sato et al. This article is distributed by The American Society for Cell Biol-ogy under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Molecular Biology of the Cell. 2018, 30(1), p. 56-68
DC.title	Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche
DC.creator	Sato, Yuya
	Kiyozumi, Daiji
	Futaki, Sugiko
	Nakano, Itsuko
	Shimono, Chisei
	Kaneko, Naoko
	Ikawa, Masahito
	Okabe, Masaru
	Sawamoto, Kazunobu
	Sekiguchi, Kiyotoshi
DC.publisher	American Society for Cell Biology
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-10
DC.identifier	https://doi.org/10.1091/mbc.E18-05-0286
DC.description	Yuya Sato, Daiji Kiyozumi, Sugiko Futaki, Itsuko Nakano, Chisei Shimono, Naoko Kaneko, Masahito Ikawa, Masaru Okabe, Kazunobu Sawamoto, and Kiyotoshi Sekiguchi, "Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche", Molecular Biology of the Cell, Vol. 30, No.1, 56-68, American Society for Cell Biology, 2018
DCTERMS.abstract	Neural stem cells (NSCs) are retained in the adult ventricular–subventricular zone (V-SVZ), a specialized neurogenic niche with a unique cellular architecture. It currently remains unclear whether or how NSCs utilize basement membranes (BMs) in this niche. Here, we examine the molecular compositions and functions of BMs in the adult mouse V-SVZ. Whole-mount V-SVZ immunostaining revealed that fractones, which are fingerlike processes of extravascular BMs, are speckled BMs unconnected to the vasculature, and differ in their molecular composition from vascular BMs. Glial fibrillary acidic protein (GFAP)-positive astrocytes and NSCs produce and adhere to speckled BMs. Furthermore, Gfap-Cre-mediated Lamc1flox(E1605Q) knockin mice, in which integrin-binding activities of laminins are specifically nullified in GFAP-positive cells, exhibit a decreased number and size of speckled BMs and reduced in vitro neurosphere-forming activity. Our results reveal niche activities of fractones/speckled BMs for NSCs and provide molecular insights into how laminin–integrin interactions regulate NSCs in vivo.
DC.rights	© 2019 Sato et al. This article is distributed by The American Society for Cell Biol-ogy under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
DC.format	71809
citation_title	Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche
citation_author	Sato, Yuya
	Kiyozumi, Daiji
	Futaki, Sugiko
	Nakano, Itsuko
	Shimono, Chisei
	Kaneko, Naoko
	Ikawa, Masahito
	Okabe, Masaru
	Sawamoto, Kazunobu
	Sekiguchi, Kiyotoshi
citation_publisher	American Society for Cell Biology
citation_language	英語
citation_date	2018-10
citation_journal_title	Molecular Biology of the Cell
citation_volume	30
citation_issue	1
citation_firstpage	56
citation_lastpage	68
citation_doi	https://doi.org/10.1091/mbc.E18-05-0286
citation_public_url	https://hdl.handle.net/11094/71809

Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche

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