Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality

Kiyozumi, Daiji; Taniguchi, Yukimasa; Nakano, Itsuko; Toga, Junko; Yagi, Emiko; Hasuwa, Hidetoshi; Ikawa, Masahito; Sekiguchi, Kiyotoshi

doi:https://doi.org/10.26508/lsa.201800064

アクセス数:250件（2024-11-05 05:13 集計）

固定URL: https://hdl.handle.net/11094/71810

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タイトル	Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality
著者	NRID 1000070452430 1000070452430 ORCID 0000-0003-3289-227X 0000-0003-3289-227X researchmap permalink dkiyozumi dkiyozumi Scopus Author ID 6506341053 6506341053 著者名 Kiyozumi, Daiji
	著者名 Taniguchi, Yukimasa
	著者名 Nakano, Itsuko
	著者名 Toga, Junko
	著者名 Yagi, Emiko
	NRID 1000050343249 1000050343249 著者名 Hasuwa, Hidetoshi
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 著者名 Sekiguchi, Kiyotoshi
内容	Daiji Kiyozumi, Yukimasa Taniguchi, Itsuko Nakano, Junko Toga, Emiko Yagi, Hidetoshi Hasuwa, Masahito Ikawa, and Kiyotoshi Sekiguchi, "Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality", Life Science Alliance, Vol.1, No.5, e201800064, Life Science Alliance, 2018
抄録	Laminin–integrin interactions regulate various adhesion-dependent cellular processes. γ1C-Glu, the Glu residue in the laminin γ1 chain C-terminal tail, is crucial for the binding of γ1-laminins to several integrin isoforms. Here, we investigated the impact of γ1C Glu to Gln mutation on γ1-laminin binding to all possible integrin partners in vitro, and found that the mutation specifically ablated binding to α3, α6, and α7 integrins. To examine the physiological significance of γ1C-Glu, we generated a knock-in allele, Lamc1EQ, in which the γ1C Glu to Gln mutation was introduced. Although Lamc1EQ/EQ homozygotes developed into blastocysts and deposited laminins in their basement membranes, they died just after implantation because of disordered extraembryonic development. Given the impact of the Lamc1EQ allele on embryonic development, we developed a knock-in mouse strain enabling on-demand introduction of the γ1C Glu to Gln mutation by the Cre-loxP system. The present study has revealed a crucial role of γ1C-Glu–mediated integrin binding in postimplantation development and provides useful animal models for investigating the physiological roles of laminin–integrin interactions in vivo.
出版者	Life Science Alliance
収録物名	Life Science Alliance
巻(号)	1(5)
ページ	e201800064
刊行年月	2018-09
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71810
eISSN	25751077
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.26508/lsa.201800064
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Life Science Alliance. 2018, 1(5), p. e201800064
DC.title	Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality
DC.creator	Kiyozumi, Daiji
	Taniguchi, Yukimasa
	Nakano, Itsuko
	Toga, Junko
	Yagi, Emiko
	Hasuwa, Hidetoshi
	Ikawa, Masahito
	Sekiguchi, Kiyotoshi
DC.publisher	Life Science Alliance
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-09
DC.identifier	https://doi.org/10.26508/lsa.201800064
DC.description	Daiji Kiyozumi, Yukimasa Taniguchi, Itsuko Nakano, Junko Toga, Emiko Yagi, Hidetoshi Hasuwa, Masahito Ikawa, and Kiyotoshi Sekiguchi, "Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality", Life Science Alliance, Vol.1, No.5, e201800064, Life Science Alliance, 2018
DCTERMS.abstract	Laminin–integrin interactions regulate various adhesion-dependent cellular processes. γ1C-Glu, the Glu residue in the laminin γ1 chain C-terminal tail, is crucial for the binding of γ1-laminins to several integrin isoforms. Here, we investigated the impact of γ1C Glu to Gln mutation on γ1-laminin binding to all possible integrin partners in vitro, and found that the mutation specifically ablated binding to α3, α6, and α7 integrins. To examine the physiological significance of γ1C-Glu, we generated a knock-in allele, Lamc1EQ, in which the γ1C Glu to Gln mutation was introduced. Although Lamc1EQ/EQ homozygotes developed into blastocysts and deposited laminins in their basement membranes, they died just after implantation because of disordered extraembryonic development. Given the impact of the Lamc1EQ allele on embryonic development, we developed a knock-in mouse strain enabling on-demand introduction of the γ1C Glu to Gln mutation by the Cre-loxP system. The present study has revealed a crucial role of γ1C-Glu–mediated integrin binding in postimplantation development and provides useful animal models for investigating the physiological roles of laminin–integrin interactions in vivo.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	71810
citation_title	Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality
citation_author	Kiyozumi, Daiji
	Taniguchi, Yukimasa
	Nakano, Itsuko
	Toga, Junko
	Yagi, Emiko
	Hasuwa, Hidetoshi
	Ikawa, Masahito
	Sekiguchi, Kiyotoshi
citation_publisher	Life Science Alliance
citation_language	英語
citation_date	2018-09
citation_journal_title	Life Science Alliance
citation_volume	1
citation_issue	5
citation_firstpage	e201800064
citation_doi	https://doi.org/10.26508/lsa.201800064
citation_public_url	https://hdl.handle.net/11094/71810

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