Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8

Fujisaki, Hitomi; Futaki, Sugiko; Yamada, Masashi; Sekiguchi, Kiyotoshi; Hayashi, Toshihiko; Ikejima, Takashi; Hattori, Shunji

doi:https://doi.org/10.1016/j.reth.2018.04.001

アクセス数:244件（2024-07-16 06:38 集計）

固定URL: https://hdl.handle.net/11094/71811

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タイトル	Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8
著者	著者名 Fujisaki, Hitomi
	NRID 1000000403014 1000000403014 著者名 Futaki, Sugiko
	NRID 1000090304055 1000090304055 著者名 Yamada, Masashi
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 著者名 Sekiguchi, Kiyotoshi
	著者名 Hayashi, Toshihiko
	著者名 Ikejima, Takashi
	著者名 Hattori, Shunji
キーワード等	Type I collagen gel
	Keratinocyte proliferation
	Calcium concentration
内容	Hitomi Fujisaki, SugikoFutaki, Masashi Yamada, Kiyotoshi Sekiguchi, Toshihiko Hayashi, Takashi Ikejima, Shunji Hattori, "Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8", Regenerative Therapy, Vol. 8, 73-79, Production and Hosting by Elsevier B.V. on behalf of the Japanese Society for Regenerative Medicine, 2018
抄録	Keratinocyte line cells HaCaT and FEPE1L-8 are used for skin model with type I collagen fibrils (gels). For this purpose, not only differentiation but also regulation of proliferation on type I collagen gels by exogenous calcium concentration is important. When exogenous calcium concentration is low, primary keratinocyte proliferation is repressed and eventually cells are induced to apoptosis on type I collagen gels. The apoptosis induced on type I collagen gels is suppressed by increasing calcium concentration in the medium. That is, higher exogenous calcium concentration is necessary for primary keratinocyte survival on type I collagen gels than for that on dish surface culture. Meanwhile much higher exogenous calcium causes cell differentiation and inhibition of proliferation. The optimal calcium concentrations for proliferation on type I collagen gels have not been clarified in keratinocyte line cells. HaCaT cells have a unique calcium sensitivity in comparison with primary keratinocytes, whereas FEPE1L-8 cells have a similar sensitivity to primary keratinocytes. In this study, we compared the effect of calcium concentrations on proliferation of HaCaT and FEPE1L-8 cells on type I collagen gels. On type I collagen gels, both line cells required higher calcium concentrations for proliferation than on dish surface. HaCaT cells proliferated better in a wider range of calcium concentrations than FEPE1L-8 cells.
出版者	Elsevier
収録物名	Regenerative Therapy
巻(号)	8
ページ	73-79
刊行年月	2018-06
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71811
eISSN	23523204
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.reth.2018.04.001
アクセス権	open access
権利情報	© 2018 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Regenerative Therapy. 2018, 8, p. 73-79
DC.title	Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8
DC.creator	Fujisaki, Hitomi
	Futaki, Sugiko
	Yamada, Masashi
	Sekiguchi, Kiyotoshi
	Hayashi, Toshihiko
	Ikejima, Takashi
	Hattori, Shunji
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-06
DC.identifier	https://doi.org/10.1016/j.reth.2018.04.001
DC.subject	Type I collagen gel
	Keratinocyte proliferation
	Calcium concentration
DC.description	Hitomi Fujisaki, SugikoFutaki, Masashi Yamada, Kiyotoshi Sekiguchi, Toshihiko Hayashi, Takashi Ikejima, Shunji Hattori, "Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8", Regenerative Therapy, Vol. 8, 73-79, Production and Hosting by Elsevier B.V. on behalf of the Japanese Society for Regenerative Medicine, 2018
DCTERMS.abstract	Keratinocyte line cells HaCaT and FEPE1L-8 are used for skin model with type I collagen fibrils (gels). For this purpose, not only differentiation but also regulation of proliferation on type I collagen gels by exogenous calcium concentration is important. When exogenous calcium concentration is low, primary keratinocyte proliferation is repressed and eventually cells are induced to apoptosis on type I collagen gels. The apoptosis induced on type I collagen gels is suppressed by increasing calcium concentration in the medium. That is, higher exogenous calcium concentration is necessary for primary keratinocyte survival on type I collagen gels than for that on dish surface culture. Meanwhile much higher exogenous calcium causes cell differentiation and inhibition of proliferation. The optimal calcium concentrations for proliferation on type I collagen gels have not been clarified in keratinocyte line cells. HaCaT cells have a unique calcium sensitivity in comparison with primary keratinocytes, whereas FEPE1L-8 cells have a similar sensitivity to primary keratinocytes. In this study, we compared the effect of calcium concentrations on proliferation of HaCaT and FEPE1L-8 cells on type I collagen gels. On type I collagen gels, both line cells required higher calcium concentrations for proliferation than on dish surface. HaCaT cells proliferated better in a wider range of calcium concentrations than FEPE1L-8 cells.
DC.rights	© 2018 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	71811
citation_title	Respective optimal calcium concentrations for proliferation on type I collagen fibrils in two keratinocyte line cells, HaCaT and FEPE1L-8
citation_author	Fujisaki, Hitomi
	Futaki, Sugiko
	Yamada, Masashi
	Sekiguchi, Kiyotoshi
	Hayashi, Toshihiko
	Ikejima, Takashi
	Hattori, Shunji
citation_publisher	Elsevier
citation_language	英語
citation_date	2018-06
citation_journal_title	Regenerative Therapy
citation_volume	8
citation_firstpage	73
citation_lastpage	79
citation_doi	https://doi.org/10.1016/j.reth.2018.04.001
citation_public_url	https://hdl.handle.net/11094/71811

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