Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation

Doi, Daisuke; Samata, Bumpei; Katsukawa, Mitsuko; Kikuchi, Tetsuhiro; Morizane, Asuka; Ono, Yuichi; Sekiguchi, Kiyotoshi; Nakagawa, Masato; Parmar, Malin; Takahashi, Jun

doi:https://doi.org/10.1016/j.stemcr.2014.01.013

アクセス数:287件（2025-01-03 07:50 集計）

固定URL: https://hdl.handle.net/11094/71813

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タイトル	Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation
著者	著者名 Doi, Daisuke
	NRID 1000080779166 1000080779166 著者名 Samata, Bumpei
	著者名 Katsukawa, Mitsuko
	著者名 Kikuchi, Tetsuhiro
	NRID 1000010528730 1000010528730 著者名 Morizane, Asuka
	著者名 Ono, Yuichi
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 著者名 Sekiguchi, Kiyotoshi
	NRID 1000010379539 1000010379539 著者名 Nakagawa, Masato
	著者名 Parmar, Malin
	NRID 1000010270779 1000010270779 著者名 Takahashi, Jun
内容	Daisuke Doi, Bumpei Samata, Mitsuko Katsukawa, Tetsuhiro Kikuchi, Asuka Morizane, Yuichi Ono, Kiyotoshi Sekiguchi, Masato Nakagawa, Malin Parmar, and Jun Takahashi, "Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation", Stem Cell Reports, 2, 3, 337-350, Cell Press, 2014
抄録	Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson’s disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN+ cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN+ cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor behavior, without tumor formation. In particular, the CORIN+ cells in a NURR1+ cell-dominant stage exhibited the best survival and function as DA neurons. Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application.
出版者	Cell Press
収録物名	Stem Cell Reports
巻(号)	2(3)
ページ	337-350
刊行年月	2014-03
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71813
eISSN	22136711
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.stemcr.2014.01.013
アクセス権	open access
権利情報	© 2014 The Authors. Published by Elsevier Inc. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Stem Cell Reports. 2014, 2(3), p. 337-350
DC.title	Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation
DC.creator	Doi, Daisuke
	Samata, Bumpei
	Katsukawa, Mitsuko
	Kikuchi, Tetsuhiro
	Morizane, Asuka
	Ono, Yuichi
	Sekiguchi, Kiyotoshi
	Nakagawa, Masato
	Parmar, Malin
	Takahashi, Jun
DC.publisher	Cell Press
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2014-03
DC.identifier	https://doi.org/10.1016/j.stemcr.2014.01.013
DC.description	Daisuke Doi, Bumpei Samata, Mitsuko Katsukawa, Tetsuhiro Kikuchi, Asuka Morizane, Yuichi Ono, Kiyotoshi Sekiguchi, Masato Nakagawa, Malin Parmar, and Jun Takahashi, "Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation", Stem Cell Reports, 2, 3, 337-350, Cell Press, 2014
DCTERMS.abstract	Human induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson’s disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN+ cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN+ cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor behavior, without tumor formation. In particular, the CORIN+ cells in a NURR1+ cell-dominant stage exhibited the best survival and function as DA neurons. Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application.
DC.rights	© 2014 The Authors. Published by Elsevier Inc. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
DC.format	71813
citation_title	Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation
citation_author	Doi, Daisuke
	Samata, Bumpei
	Katsukawa, Mitsuko
	Kikuchi, Tetsuhiro
	Morizane, Asuka
	Ono, Yuichi
	Sekiguchi, Kiyotoshi
	Nakagawa, Masato
	Parmar, Malin
	Takahashi, Jun
citation_publisher	Cell Press
citation_language	英語
citation_date	2014-03
citation_journal_title	Stem Cell Reports
citation_volume	2
citation_issue	3
citation_firstpage	337
citation_lastpage	350
citation_doi	https://doi.org/10.1016/j.stemcr.2014.01.013
citation_public_url	https://hdl.handle.net/11094/71813

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