CD151 regulates epithelial cell–cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization

Shigeta, Masaki; Sanzen, Noriko; Ozawa, Masayuki; Gu, Jianguo; Hasegawa, Hitoshi; Sekiguchi, Kiyotoshi

doi:https://doi.org/10.1083/jcb.200301075

CD151 regulates epithelial cell–cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization

Shigeta, Masaki; Sanzen, Noriko; Ozawa, Masayuki et al.

Journal of Cell Biology, 2003, 163(1), 165-176

アクセス数:581件（2025-07-08 17:06 集計）

固定URL: https://hdl.handle.net/11094/71819

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タイトル	CD151 regulates epithelial cell–cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization
著者	著者名 Shigeta, Masaki
	著者名 Sanzen, Noriko
	NRID 1000090136854 1000090136854 著者名 Ozawa, Masayuki
	NRID 1000040260369 1000040260369 著者名 Gu, Jianguo
	NRID 1000040164826 1000040164826 著者名 Hasegawa, Hitoshi
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 著者名 Sekiguchi, Kiyotoshi
キーワード等	cell adhesion
	tetraspanin
	integrin
	actin cytoskeleton
	Rho family GTPase
内容	© 2003 Rockefeller University Press. Originally published in the Journal of Cell Biology. https://doi.org/10.1083/jcb.200301075
抄録	CD151, a member of the tetraspanin family proteins, tightly associates with integrin α3β1 and localizes at basolateral surfaces of epithelial cells. We found that overexpression of CD151 in A431 cells accelerated intercellular adhesion, whereas treatment of cells with anti-CD151 mAb perturbed the integrity of cortical actin filaments and cell polarity. E-Cadherin puncta formation, indicative of filopodia-based adhesion zipper formation, as well as E-cadherin anchorage to detergent-insoluble cytoskeletal matrix, was enhanced in CD151-overexpressing cells. Levels of GTP-bound Cdc42 and Rac were also elevated in CD151-overexpressing cells, suggesting the role of CD151 in E-cadherin–mediated cell–cell adhesion as a modulator of actin cytoskeletal reorganization. Consistent with this possibility, engagement of CD151 by the substrate-adsorbed anti-CD151 mAb induced prominent Cdc42-dependent filopodial extension, which along with E-cadherin puncta formation, was strongly inhibited by calphostin C, a protein kinase C (PKC) inhibitor. Together, these results indicate that CD151 is involved in epithelial cell–cell adhesion as a modulator of PKC- and Cdc42-dependent actin cytoskeletal reorganization.
出版者	Rockefeller University Press
収録物名	Journal of Cell Biology
巻(号)	163(1)
ページ	165-176
刊行年月	2003-10
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71819
PISSN	00219525
eISSN	15408140
NCID	AA00694812
NCID	AA1203766X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1083/jcb.200301075
アクセス権	open access
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Cell Biology. 2003, 163(1), p. 165-176
DC.title	CD151 regulates epithelial cell–cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization
DC.creator	Shigeta, Masaki
	Sanzen, Noriko
	Ozawa, Masayuki
	Gu, Jianguo
	Hasegawa, Hitoshi
	Sekiguchi, Kiyotoshi
DC.publisher	Rockefeller University Press
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2003-10
DC.identifier	https://doi.org/10.1083/jcb.200301075
DC.subject	cell adhesion
	tetraspanin
	integrin
	actin cytoskeleton
	Rho family GTPase
DC.description	© 2003 Rockefeller University Press. Originally published in the Journal of Cell Biology. https://doi.org/10.1083/jcb.200301075
DCTERMS.abstract	CD151, a member of the tetraspanin family proteins, tightly associates with integrin α3β1 and localizes at basolateral surfaces of epithelial cells. We found that overexpression of CD151 in A431 cells accelerated intercellular adhesion, whereas treatment of cells with anti-CD151 mAb perturbed the integrity of cortical actin filaments and cell polarity. E-Cadherin puncta formation, indicative of filopodia-based adhesion zipper formation, as well as E-cadherin anchorage to detergent-insoluble cytoskeletal matrix, was enhanced in CD151-overexpressing cells. Levels of GTP-bound Cdc42 and Rac were also elevated in CD151-overexpressing cells, suggesting the role of CD151 in E-cadherin–mediated cell–cell adhesion as a modulator of actin cytoskeletal reorganization. Consistent with this possibility, engagement of CD151 by the substrate-adsorbed anti-CD151 mAb induced prominent Cdc42-dependent filopodial extension, which along with E-cadherin puncta formation, was strongly inhibited by calphostin C, a protein kinase C (PKC) inhibitor. Together, these results indicate that CD151 is involved in epithelial cell–cell adhesion as a modulator of PKC- and Cdc42-dependent actin cytoskeletal reorganization.
DC.format	71819
citation_title	CD151 regulates epithelial cell–cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization
citation_author	Shigeta, Masaki
	Sanzen, Noriko
	Ozawa, Masayuki
	Gu, Jianguo
	Hasegawa, Hitoshi
	Sekiguchi, Kiyotoshi
citation_publisher	Rockefeller University Press
citation_language	英語
citation_date	2003-10
citation_journal_title	Journal of Cell Biology
citation_volume	163
citation_issue	1
citation_firstpage	165
citation_lastpage	176
citation_doi	https://doi.org/10.1083/jcb.200301075
citation_public_url	https://hdl.handle.net/11094/71819

CD151 regulates epithelial cell–cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization

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