Laminin γ2 fragments are increased in the circulation of patients with early phase acute lung injury

Katayama, Masahiko; Ishizaka, Akitoshi; Sakamoto, Michiie; Fujishima, Seitaro; Sekiguchi, Kiyotoshi; Asano, Koichiro; Betsuyaku, Tomoko; Kotani, Toru; Ware, Lorraine B.; Matthay, Michael A.; Hashimoto, Satoru

doi:https://doi.org/10.1007/s00134-009-1719-6

Laminin γ2 fragments are increased in the circulation of patients with early phase acute lung injury

Katayama, Masahiko; Ishizaka, Akitoshi; Sakamoto, Michiie et al.

Intensive Care Medicine, 2010, 36(3), 479-486

アクセス数:658件（2025-10-24 09:04 集計）

固定URL: https://hdl.handle.net/11094/71821

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タイトル	Laminin γ2 fragments are increased in the circulation of patients with early phase acute lung injury
著者	著者名 Katayama, Masahiko
	NRID 1000090176181 1000090176181 著者名 Ishizaka, Akitoshi
	NRID 1000040221270 1000040221270 著者名 Sakamoto, Michiie
	NRID 1000000173419 1000000173419 著者名 Fujishima, Seitaro
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 著者名 Sekiguchi, Kiyotoshi
	著者名 Asano, Koichiro
	NRID 1000060333605 1000060333605 著者名 Betsuyaku, Tomoko
	著者名 Kotani, Toru
	著者名 Ware, Lorraine B.
	著者名 Matthay, Michael A.
	NRID 1000090167578 1000090167578 著者名 Hashimoto, Satoru
キーワード等	Laminin
	Circulation
	Acute lung injury
	Alveolar epithelial repair
内容	Katayama, M., Ishizaka, A., Sakamoto, M. et al. Intensive Care Med (2010) 36: 479. https://doi.org/10.1007/s00134-009-1719-6
抄録	Objective: Laminin-5, a cell adhesive molecule expressed solely by epithelium, is known to enhance epithelial cell migration and repair of injured epithelium, after its essential component γ2-chain is processed proteolytically. Our previous study revealed circulating levels of amino-terminal fragment of laminin γ2-chain (G2F) reflect epithelial tumor invasiveness in carcinoma patients, but its physiological role in alveolar epithelial injury remains unknown.
	Design: Sampling of epithelial lining fluids or pulmonary edema fluids from patients with acute lung injury (ALI) or related diseases was performed. Plasma samples were obtained from them at the time of disease onset or later. G2F concentrations were determined by immunoassay constructed by ourselves.
	Results: We found a significantly higher amount of G2F in pulmonary edema and epithelial lining fluids of patients with ALI, as compared with those with the other respiratory diseases. Their plasma levels were also elevated significantly early at the onset of ALI (mean ± SD; 147 ± 82 ng/ml in non-surviving and 90 ± 56 in surviving patients) as compared with those in the patients with cardiogenic pulmonary edema (59 ± 36) or idiopathic pulmonary fibrosis (37 ± 17), indicating alveolar epithelium rapidly secrete laminin-5 in ALI. At 5 days after onset, non-surviving patients maintained higher plasma concentrations (152 ± 84), but in contrast, the levels in surviving patients declined (71 ± 35), suggesting secretion of laminin-5 was suppressed, associated with recovery from ALI.
	Conclusion: Circulating G2F may be a biomarker for alveolar laminin-5 secreted early at disease onset in ALI, potentially regulating alveolar re-epithelialization.
出版者	Springer Nature
収録物名	Intensive Care Medicine
巻(号)	36(3)
ページ	479-486
刊行年月	2010-03
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71821
PISSN	03424642
eISSN	14321238
NCID	AA00676661
NCID	AA11625287
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1007/s00134-009-1719-6
アクセス権	open access
権利情報	© 2009 The Author(s). This article is licensed under a Creative Commons Attribution-NonCommercial 2.0 Generic License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Intensive Care Medicine. 2010, 36(3), p. 479-486
DC.title	Laminin γ2 fragments are increased in the circulation of patients with early phase acute lung injury
DC.creator	Katayama, Masahiko
	Ishizaka, Akitoshi
	Sakamoto, Michiie
	Fujishima, Seitaro
	Sekiguchi, Kiyotoshi
	Asano, Koichiro
	Betsuyaku, Tomoko
	Kotani, Toru
	Ware, Lorraine B.
	Matthay, Michael A.
	Hashimoto, Satoru
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2010-03
DC.identifier	https://doi.org/10.1007/s00134-009-1719-6
DC.subject	Laminin
	Circulation
	Acute lung injury
	Alveolar epithelial repair
DC.description	Katayama, M., Ishizaka, A., Sakamoto, M. et al. Intensive Care Med (2010) 36: 479. https://doi.org/10.1007/s00134-009-1719-6
DCTERMS.abstract	Objective: Laminin-5, a cell adhesive molecule expressed solely by epithelium, is known to enhance epithelial cell migration and repair of injured epithelium, after its essential component γ2-chain is processed proteolytically. Our previous study revealed circulating levels of amino-terminal fragment of laminin γ2-chain (G2F) reflect epithelial tumor invasiveness in carcinoma patients, but its physiological role in alveolar epithelial injury remains unknown.
	Design: Sampling of epithelial lining fluids or pulmonary edema fluids from patients with acute lung injury (ALI) or related diseases was performed. Plasma samples were obtained from them at the time of disease onset or later. G2F concentrations were determined by immunoassay constructed by ourselves.
	Results: We found a significantly higher amount of G2F in pulmonary edema and epithelial lining fluids of patients with ALI, as compared with those with the other respiratory diseases. Their plasma levels were also elevated significantly early at the onset of ALI (mean ± SD; 147 ± 82 ng/ml in non-surviving and 90 ± 56 in surviving patients) as compared with those in the patients with cardiogenic pulmonary edema (59 ± 36) or idiopathic pulmonary fibrosis (37 ± 17), indicating alveolar epithelium rapidly secrete laminin-5 in ALI. At 5 days after onset, non-surviving patients maintained higher plasma concentrations (152 ± 84), but in contrast, the levels in surviving patients declined (71 ± 35), suggesting secretion of laminin-5 was suppressed, associated with recovery from ALI.
	Conclusion: Circulating G2F may be a biomarker for alveolar laminin-5 secreted early at disease onset in ALI, potentially regulating alveolar re-epithelialization.
DC.rights	© 2009 The Author(s). This article is licensed under a Creative Commons Attribution-NonCommercial 2.0 Generic License.
DC.format	71821
citation_title	Laminin γ2 fragments are increased in the circulation of patients with early phase acute lung injury
citation_author	Katayama, Masahiko
	Ishizaka, Akitoshi
	Sakamoto, Michiie
	Fujishima, Seitaro
	Sekiguchi, Kiyotoshi
	Asano, Koichiro
	Betsuyaku, Tomoko
	Kotani, Toru
	Ware, Lorraine B.
	Matthay, Michael A.
	Hashimoto, Satoru
citation_publisher	Springer Nature
citation_language	英語
citation_date	2010-03
citation_journal_title	Intensive Care Medicine
citation_volume	36
citation_issue	3
citation_firstpage	479
citation_lastpage	486
citation_doi	https://doi.org/10.1007/s00134-009-1719-6
citation_public_url	https://hdl.handle.net/11094/71821

Laminin γ2 fragments are increased in the circulation of patients with early phase acute lung injury

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