Laminin isoforms in human embryonic stem cells : synthesis, receptor usage and growth support

Vuoristo, Sanna; Virtanen, Ismo; Takkunen, Minna; Palgi, Jaan; Kikkawa, Yamato; Rousselle, Patricia; Sekiguchi, Kiyotoshi; Tuuri, Timo; Otonkoski, Timo

doi:https://doi.org/10.1111/j.1582-4934.2008.00643.x

Laminin isoforms in human embryonic stem cells : synthesis, receptor usage and growth support

Vuoristo, Sanna; Virtanen, Ismo; Takkunen, Minna et al.

Journal of Cellular and Molecular Medicine, 2009, 13(8b), 2622-2633

アクセス数:571件（2025-10-24 09:23 集計）

固定URL: https://hdl.handle.net/11094/71822

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タイトル	Laminin isoforms in human embryonic stem cells : synthesis, receptor usage and growth support
著者	著者名 Vuoristo, Sanna
	著者名 Virtanen, Ismo
	著者名 Takkunen, Minna
	著者名 Palgi, Jaan
	NRID 1000020274227 1000020274227 著者名 Kikkawa, Yamato
	著者名 Rousselle, Patricia
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 著者名 Sekiguchi, Kiyotoshi
	著者名 Tuuri, Timo
	著者名 Otonkoski, Timo
キーワード等	human embryonic stem cell
	extracellular matrix
	basement membrane
	laminin
	integrin
	B‐CAM/Lutheran
	adhesion
	defined cultures
内容	Sanna Vuoristo, Ismo Virtanen, Minna Takkunen, Jaan Palgi, Yamato Kikkawa, Patricia Rousselle, Kiyotoshi Sekiguchi, Timo Tuuri, and Timo Otonkoski, "Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support", Journal of Cellular and Molecular Medicine, 13, 8b, pp. 2622-2633, Wiley, 2009
抄録	To reveal the functional intrinsic niche of human embryonic stem cells (hESC) we examined the production of basement membrane (BM) proteins and the presence of their receptors in feeder‐free cell culture conditions. In addition, we investigated binding of hESCs to purified human BM proteins and identified the receptors mediating these contacts. Also, we tested whether purified human laminin (Lm) isoforms have a role in hESC self‐renewal and growth in short‐term cultures. The results show that hESCs synthesize Lm α1 and Lm α5 chains together with Lm β1 and γ1 chains suggesting the production of Lms‐111 and ‐511 into the culture medium and deposits on cells. hESCs contain functionally important integrin (Int) subunits, Int β1, α3, α6, α5, β5 and αV, as well as the Lm α5 receptor, Lutheran (Lu) glycoprotein and its truncated form, basal cell adhesion molecule (B‐CAM). In cell adhesion experiments, Int β1 was crucial for adhesion to most of the purified human BM proteins. Lu/B‐CAM mediated adhesion to Lm‐511 together with Int α3β1, and was essential for the adhesion of hESCs to embryonic feeder cells. Adhesion to Lm‐411 was mediated by Int α6β1. Lm‐511 supported hESC growth in defined medium equally well as Matrigel. These results provide consequential information of the biological role of BM in hESCs, warranting further investigation of BM biology of human pluripotent stem cells.
出版者	Wiley
収録物名	Journal of Cellular and Molecular Medicine
巻(号)	13(8b)
ページ	2622-2633
刊行年月	2009-08
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71822
eISSN	15824934
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1111/j.1582-4934.2008.00643.x
アクセス権	open access
権利情報	© 2008 The Authors Journal compilation. © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Cellular and Molecular Medicine. 2009, 13(8b), p. 2622-2633
DC.title	Laminin isoforms in human embryonic stem cells : synthesis, receptor usage and growth support
DC.creator	Vuoristo, Sanna
	Virtanen, Ismo
	Takkunen, Minna
	Palgi, Jaan
	Kikkawa, Yamato
	Rousselle, Patricia
	Sekiguchi, Kiyotoshi
	Tuuri, Timo
	Otonkoski, Timo
DC.publisher	Wiley
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2009-08
DC.identifier	https://doi.org/10.1111/j.1582-4934.2008.00643.x
DC.subject	human embryonic stem cell
	extracellular matrix
	basement membrane
	laminin
	integrin
	B‐CAM/Lutheran
	adhesion
	defined cultures
DC.description	Sanna Vuoristo, Ismo Virtanen, Minna Takkunen, Jaan Palgi, Yamato Kikkawa, Patricia Rousselle, Kiyotoshi Sekiguchi, Timo Tuuri, and Timo Otonkoski, "Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support", Journal of Cellular and Molecular Medicine, 13, 8b, pp. 2622-2633, Wiley, 2009
DCTERMS.abstract	To reveal the functional intrinsic niche of human embryonic stem cells (hESC) we examined the production of basement membrane (BM) proteins and the presence of their receptors in feeder‐free cell culture conditions. In addition, we investigated binding of hESCs to purified human BM proteins and identified the receptors mediating these contacts. Also, we tested whether purified human laminin (Lm) isoforms have a role in hESC self‐renewal and growth in short‐term cultures. The results show that hESCs synthesize Lm α1 and Lm α5 chains together with Lm β1 and γ1 chains suggesting the production of Lms‐111 and ‐511 into the culture medium and deposits on cells. hESCs contain functionally important integrin (Int) subunits, Int β1, α3, α6, α5, β5 and αV, as well as the Lm α5 receptor, Lutheran (Lu) glycoprotein and its truncated form, basal cell adhesion molecule (B‐CAM). In cell adhesion experiments, Int β1 was crucial for adhesion to most of the purified human BM proteins. Lu/B‐CAM mediated adhesion to Lm‐511 together with Int α3β1, and was essential for the adhesion of hESCs to embryonic feeder cells. Adhesion to Lm‐411 was mediated by Int α6β1. Lm‐511 supported hESC growth in defined medium equally well as Matrigel. These results provide consequential information of the biological role of BM in hESCs, warranting further investigation of BM biology of human pluripotent stem cells.
DC.rights	© 2008 The Authors Journal compilation. © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
DC.format	71822
citation_title	Laminin isoforms in human embryonic stem cells : synthesis, receptor usage and growth support
citation_author	Vuoristo, Sanna
	Virtanen, Ismo
	Takkunen, Minna
	Palgi, Jaan
	Kikkawa, Yamato
	Rousselle, Patricia
	Sekiguchi, Kiyotoshi
	Tuuri, Timo
	Otonkoski, Timo
citation_publisher	Wiley
citation_language	英語
citation_date	2009-08
citation_journal_title	Journal of Cellular and Molecular Medicine
citation_volume	13
citation_issue	8b
citation_firstpage	2622
citation_lastpage	2633
citation_doi	https://doi.org/10.1111/j.1582-4934.2008.00643.x
citation_public_url	https://hdl.handle.net/11094/71822

Laminin isoforms in human embryonic stem cells : synthesis, receptor usage and growth support

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