Membrane Type 1 Matrix Metalloprotease Cleaves Laminin-10 and Promotes Prostate Cancer Cell Migration

Bair, Elisabeth L.; Chen, Man Ling; McDaniel, Kathy; Sekiguchi, Kiyotoshi; Cress, Anne E.; Nagle, Raymond B.; Bowden, George Timothy

doi:https://doi.org/10.1593/neo.04619

Membrane Type 1 Matrix Metalloprotease Cleaves Laminin-10 and Promotes Prostate Cancer Cell Migration

Bair, Elisabeth L.; Chen, Man Ling; McDaniel, Kathy et al.

Neoplasia, 2005, 7(4), 380-389

アクセス数:618件（2025-08-15 23:06 集計）

固定URL: https://hdl.handle.net/11094/71825

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タイトル	Membrane Type 1 Matrix Metalloprotease Cleaves Laminin-10 and Promotes Prostate Cancer Cell Migration
著者	著者名 Bair, Elisabeth L.
	著者名 Chen, Man Ling
	著者名 McDaniel, Kathy
	NRID 1000050187845 1000050187845 researchmap permalink read0054833 read0054833 Scopus Author ID 7201662709 7201662709 ORCID 0000-0002-6433-1410 0000-0002-6433-1410 著者名 Sekiguchi, Kiyotoshi
	著者名 Cress, Anne E.
	著者名 Nagle, Raymond B.
	著者名 Bowden, George Timothy
キーワード等	Laminin-10
	MT1-MMP
	prostate
	MMP-14
	invasion
内容	Elisabeth L. Bair, Man Ling Chen, Kathy McDaniel, Kiyotoshi Sekiguchi, Anne E. Cress, Raymond B. Nagle, and George Timothy Bowden, "Membrane Type 1 Matrix Metalloprotease Cleaves Laminin-10 and Promotes Prostate Cancer Cell Migration", Neoplasia, Vol. 7 (4), pp. 380-389, Elsevier, 2005
抄録	Disruption of the extracellular matrix by proteases is crucial for tumor invasion. Laminin-10 (Ln-10) has previously been identified as a substrate for cell migration and cell adhesion, and is present in the basal lamina (BL) of both normal prostate and prostate cancer. Here, we investigate a role for membrane type 1 matrix metalloprotease (MT1-MMP) in modifying this Ln-10-rich BL. MT1-MMP is a transmembrane member of the MMP family that has been demonstrated to be upregulated as prostate cancer progresses from normal to prostate intraepithelial neoplasia to invasive cancer, suggesting a role for MT1-MMP in the invasion of prostate cancer. We show that MT1-MMP cleaves the α5 chain of purified human Ln-10 from its 350-kDa form into 310-, 190-, 160-, and 45-kDa fragments. This cleavage causes a decrease in DU-145 prostate cancer cell adhesion to purified Ln-10, and an increase in transmigration of DU-145 cells through cleaved Ln-10. We also show that prostate cancer cells expressing membrane-bound MT1-MMP cleave the α5 chain of Ln-10. Ln α5-chain cleavage is also observed in human prostate cancer tissues. These findings suggest that prostate cancer cells expressing high levels of MT1-MMP have increased invasive potential through their ability to degrade and invade Ln-10 barriers.
出版者	Elsevier
収録物名	Neoplasia
巻(号)	7(4)
ページ	380-389
刊行年月	2005-04
言語	英語
ハンドルURL	https://hdl.handle.net/11094/71825
eISSN	14765586
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1593/neo.04619
アクセス権	open access
権利情報	© 2005 Neoplasia Press, Inc. Published by Elsevier Inc. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Neoplasia. 2005, 7(4), p. 380-389
DC.title	Membrane Type 1 Matrix Metalloprotease Cleaves Laminin-10 and Promotes Prostate Cancer Cell Migration
DC.creator	Bair, Elisabeth L.
	Chen, Man Ling
	McDaniel, Kathy
	Sekiguchi, Kiyotoshi
	Cress, Anne E.
	Nagle, Raymond B.
	Bowden, George Timothy
DC.publisher	Elsevier
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2005-04
DC.identifier	https://doi.org/10.1593/neo.04619
DC.subject	Laminin-10
	MT1-MMP
	prostate
	MMP-14
	invasion
DC.description	Elisabeth L. Bair, Man Ling Chen, Kathy McDaniel, Kiyotoshi Sekiguchi, Anne E. Cress, Raymond B. Nagle, and George Timothy Bowden, "Membrane Type 1 Matrix Metalloprotease Cleaves Laminin-10 and Promotes Prostate Cancer Cell Migration", Neoplasia, Vol. 7 (4), pp. 380-389, Elsevier, 2005
DCTERMS.abstract	Disruption of the extracellular matrix by proteases is crucial for tumor invasion. Laminin-10 (Ln-10) has previously been identified as a substrate for cell migration and cell adhesion, and is present in the basal lamina (BL) of both normal prostate and prostate cancer. Here, we investigate a role for membrane type 1 matrix metalloprotease (MT1-MMP) in modifying this Ln-10-rich BL. MT1-MMP is a transmembrane member of the MMP family that has been demonstrated to be upregulated as prostate cancer progresses from normal to prostate intraepithelial neoplasia to invasive cancer, suggesting a role for MT1-MMP in the invasion of prostate cancer. We show that MT1-MMP cleaves the α5 chain of purified human Ln-10 from its 350-kDa form into 310-, 190-, 160-, and 45-kDa fragments. This cleavage causes a decrease in DU-145 prostate cancer cell adhesion to purified Ln-10, and an increase in transmigration of DU-145 cells through cleaved Ln-10. We also show that prostate cancer cells expressing membrane-bound MT1-MMP cleave the α5 chain of Ln-10. Ln α5-chain cleavage is also observed in human prostate cancer tissues. These findings suggest that prostate cancer cells expressing high levels of MT1-MMP have increased invasive potential through their ability to degrade and invade Ln-10 barriers.
DC.rights	© 2005 Neoplasia Press, Inc. Published by Elsevier Inc. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
DC.format	71825
citation_title	Membrane Type 1 Matrix Metalloprotease Cleaves Laminin-10 and Promotes Prostate Cancer Cell Migration
citation_author	Bair, Elisabeth L.
	Chen, Man Ling
	McDaniel, Kathy
	Sekiguchi, Kiyotoshi
	Cress, Anne E.
	Nagle, Raymond B.
	Bowden, George Timothy
citation_publisher	Elsevier
citation_language	英語
citation_date	2005-04
citation_journal_title	Neoplasia
citation_volume	7
citation_issue	4
citation_firstpage	380
citation_lastpage	389
citation_doi	https://doi.org/10.1593/neo.04619
citation_public_url	https://hdl.handle.net/11094/71825

Membrane Type 1 Matrix Metalloprotease Cleaves Laminin-10 and Promotes Prostate Cancer Cell Migration

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