IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95

Kim, Hyunsoo; Takegahara, Noriko; Walsh, Matthew C.; Middleton, Sarah A.; Yu, Jiyeon; Shirakawa, Jumpei; Ueda, Jun; Fujihara, Yoshitaka; Ikawa, Masahito; Ishii, Masaru; Kim, Junhyong; Choi, Yongwon

doi:https://doi.org/10.1038/s41413-019-0080-9

IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95

Kim, Hyunsoo; Takegahara, Noriko; Walsh, Matthew C. et al.

Bone Research, 2020, 8, 5

アクセス数:246件（2025-04-18 02:11 集計）

固定URL: https://hdl.handle.net/11094/78538

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タイトル	IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95
著者	著者名 Kim, Hyunsoo
	著者名 Takegahara, Noriko
	著者名 Walsh, Matthew C.
	著者名 Middleton, Sarah A.
	著者名 Yu, Jiyeon
	著者名 Shirakawa, Jumpei
	NRID 1000080450394 1000080450394 著者名 Ueda, Jun
	NRID 1000070578848 1000070578848 著者名 Fujihara, Yoshitaka
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000010324758 1000010324758 researchmap permalink read0076684 read0076684 Scopus Author ID 35074093500 35074093500 著者名 Ishii, Masaru
	著者名 Kim, Junhyong
	著者名 Choi, Yongwon
内容	Kim, H., Takegahara, N., Walsh, M.C. et al. IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95. Bone Res 8, 5 (2020). https://doi.org/10.1038/s41413-019-0080-9
抄録	Osteoclasts are multinucleated, giant cells derived from myeloid progenitors. While receptor activator of NF-κB ligand (RANKL) stimulation is the primary driver of osteoclast differentiation, additional signaling further contributes to osteoclast maturation. Here, we demonstrate that immunoglobulin superfamily member 11 (IgSF11), whose expression increases during osteoclast differentiation, regulates osteoclast differentiation through interaction with postsynaptic density protein 95 (PSD-95), a scaffold protein with multiple protein interaction domains. IgSF11 deficiency in vivo results in impaired osteoclast differentiation and bone resorption but no observed defect in bone formation. Consequently, IgSF11-deficient mice exhibit increased bone mass. Using in vitro osteoclast culture systems, we show that IgSF11 functions through homophilic interactions. Additionally, we demonstrate that impaired osteoclast differentiation in IgSF11-deficient cells is rescued by full-length IgSF11 and that the IgSF11-PSD-95 interaction requires the 75 C-terminal amino acids of IgSF11. Our findings reveal a critical role for IgSF11 during osteoclast differentiation and suggest a role for IgSF11 in a receptor- and signal transduction molecule-containing protein complex.
出版者	Springer Nature
収録物名	Bone Research
巻(号)	8
ページ	5
刊行年月	2020-02-10
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78538
PISSN	20954700
eISSN	20956231
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41413-019-0080-9
アクセス権	open access
権利情報	© 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Bone Research. 2020, 8, p. 5
DC.title	IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95
DC.creator	Kim, Hyunsoo
	Takegahara, Noriko
	Walsh, Matthew C.
	Middleton, Sarah A.
	Yu, Jiyeon
	Shirakawa, Jumpei
	Ueda, Jun
	Fujihara, Yoshitaka
	Ikawa, Masahito
	Ishii, Masaru
	Kim, Junhyong
	Choi, Yongwon
DC.publisher	Springer Nature
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-02-10
DC.identifier	https://doi.org/10.1038/s41413-019-0080-9
DC.description	Kim, H., Takegahara, N., Walsh, M.C. et al. IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95. Bone Res 8, 5 (2020). https://doi.org/10.1038/s41413-019-0080-9
DCTERMS.abstract	Osteoclasts are multinucleated, giant cells derived from myeloid progenitors. While receptor activator of NF-κB ligand (RANKL) stimulation is the primary driver of osteoclast differentiation, additional signaling further contributes to osteoclast maturation. Here, we demonstrate that immunoglobulin superfamily member 11 (IgSF11), whose expression increases during osteoclast differentiation, regulates osteoclast differentiation through interaction with postsynaptic density protein 95 (PSD-95), a scaffold protein with multiple protein interaction domains. IgSF11 deficiency in vivo results in impaired osteoclast differentiation and bone resorption but no observed defect in bone formation. Consequently, IgSF11-deficient mice exhibit increased bone mass. Using in vitro osteoclast culture systems, we show that IgSF11 functions through homophilic interactions. Additionally, we demonstrate that impaired osteoclast differentiation in IgSF11-deficient cells is rescued by full-length IgSF11 and that the IgSF11-PSD-95 interaction requires the 75 C-terminal amino acids of IgSF11. Our findings reveal a critical role for IgSF11 during osteoclast differentiation and suggest a role for IgSF11 in a receptor- and signal transduction molecule-containing protein complex.
DC.rights	© 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78538
citation_title	IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95
citation_author	Kim, Hyunsoo
	Takegahara, Noriko
	Walsh, Matthew C.
	Middleton, Sarah A.
	Yu, Jiyeon
	Shirakawa, Jumpei
	Ueda, Jun
	Fujihara, Yoshitaka
	Ikawa, Masahito
	Ishii, Masaru
	Kim, Junhyong
	Choi, Yongwon
citation_publisher	Springer Nature
citation_language	英語
citation_date	2020-02-10
citation_journal_title	Bone Research
citation_volume	8
citation_firstpage	5
citation_doi	https://doi.org/10.1038/s41413-019-0080-9
citation_public_url	https://hdl.handle.net/11094/78538

IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95

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