PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins

Lee, Gun Hee; Fujita, Morihisa; Nakanishi, Hideki; Miyata, Haruhiko; Ikawa, Masahito; Maeda, Yusuke; Murakami, Yoshiko; Kinoshita, Taroh

doi:https://doi.org/10.1074/jbc.RA120.014643

PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins

Lee, Gun Hee; Fujita, Morihisa; Nakanishi, Hideki et al.

Journal of Biological Chemistry, 2020, 295(42), 14501-14509

アクセス数:224件（2025-04-18 02:39 集計）

固定URL: https://hdl.handle.net/11094/78540

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
JBiolChem_295_42_14501	pdf	なし	1.96 MB	185	2021.02.08

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins
著者	著者名 Lee, Gun Hee
	NRID 1000030532056 1000030532056 著者名 Fujita, Morihisa
	著者名 Nakanishi, Hideki
	NRID 1000050604732 1000050604732 researchmap permalink hmiyata hmiyata Scopus Author ID 37161837000 37161837000 ORCID 0000-0003-4758-5803 0000-0003-4758-5803 著者名 Miyata, Haruhiko
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000000294124 1000000294124 著者名 Maeda, Yusuke
	NRID 1000000304048 1000000304048 researchmap permalink read0191261 read0191261 Scopus Author ID 35725869400 35725869400 著者名 Murakami, Yoshiko
	著者名 Kinoshita, Taroh
内容	Gun-Hee Lee, Morihisa Fujita, Hideki Nakanishi, Haruhiko Miyata, Masahito Ikawa, Yusuke Maeda, Yoshiko Murakami, Taroh Kinoshita, PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins, Journal of Biological Chemistry, Volume 295, Issue 42, 2020, Pages 14501-14509, ISSN 0021-9258, https://doi.org/10.1074/jbc.RA120.014643.
抄録	PGAP6, also known as TMEM8A, is a phospholipase A2 with specificity to glycosylphosphatidylinositol (GPI) and expressed on the surface of various cells. CRIPTO, a GPI-anchored co-receptor for a morphogenic factor Nodal, is a sensitive substrate of PGAP6. PGAP6-mediated shedding of CRIPTO plays a critical role in an early stage of embryogenesis. In contrast, CRYPTIC, a close family member of CRIPTO, is resistant to PGAP6. In this report, chimeras between CRIPTO and CRYPTIC and truncate mutants of PGAP6 were used to demonstrate that the Cripto-1/FRL1/Cryptic domain of CRIPTO is recognized by an N-terminal domain of PGAP6 for processing. We also report that among 56 human GPI-anchored proteins tested, only glypican 3, prostasin, SPACA4, and contactin-1, in addition to CRIPTO, are sensitive to PGAP6, indicating that PGAP6 has a narrow specificity toward various GPI-anchored proteins.
出版者	American Society for Biochemistry and Molecular Biology Inc.
収録物名	Journal of Biological Chemistry
巻(号)	295(42)
ページ	14501-14509
刊行年月	2020-10-16
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78540
PISSN	00219258
eISSN	1083351X
NCID	AA1202441X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1074/jbc.RA120.014643
関連情報 (isIdenticalTo)	PMID 32816994
アクセス権	open access
権利情報	© 2020 Lee et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2020, 295(42), p. 14501-14509
DC.title	PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins
DC.creator	Lee, Gun Hee
	Fujita, Morihisa
	Nakanishi, Hideki
	Miyata, Haruhiko
	Ikawa, Masahito
	Maeda, Yusuke
	Murakami, Yoshiko
	Kinoshita, Taroh
DC.publisher	American Society for Biochemistry and Molecular Biology Inc.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-10-16
DC.identifier	https://doi.org/10.1074/jbc.RA120.014643
DC.description	Gun-Hee Lee, Morihisa Fujita, Hideki Nakanishi, Haruhiko Miyata, Masahito Ikawa, Yusuke Maeda, Yoshiko Murakami, Taroh Kinoshita, PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins, Journal of Biological Chemistry, Volume 295, Issue 42, 2020, Pages 14501-14509, ISSN 0021-9258, https://doi.org/10.1074/jbc.RA120.014643.
DCTERMS.abstract	PGAP6, also known as TMEM8A, is a phospholipase A2 with specificity to glycosylphosphatidylinositol (GPI) and expressed on the surface of various cells. CRIPTO, a GPI-anchored co-receptor for a morphogenic factor Nodal, is a sensitive substrate of PGAP6. PGAP6-mediated shedding of CRIPTO plays a critical role in an early stage of embryogenesis. In contrast, CRYPTIC, a close family member of CRIPTO, is resistant to PGAP6. In this report, chimeras between CRIPTO and CRYPTIC and truncate mutants of PGAP6 were used to demonstrate that the Cripto-1/FRL1/Cryptic domain of CRIPTO is recognized by an N-terminal domain of PGAP6 for processing. We also report that among 56 human GPI-anchored proteins tested, only glypican 3, prostasin, SPACA4, and contactin-1, in addition to CRIPTO, are sensitive to PGAP6, indicating that PGAP6 has a narrow specificity toward various GPI-anchored proteins.
DC.rights	© 2020 Lee et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78540
citation_title	PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins
citation_author	Lee, Gun Hee
	Fujita, Morihisa
	Nakanishi, Hideki
	Miyata, Haruhiko
	Ikawa, Masahito
	Maeda, Yusuke
	Murakami, Yoshiko
	Kinoshita, Taroh
citation_publisher	American Society for Biochemistry and Molecular Biology Inc.
citation_language	英語
citation_date	2020-10-16
citation_journal_title	Journal of Biological Chemistry
citation_volume	295
citation_issue	42
citation_firstpage	14501
citation_lastpage	14509
citation_doi	https://doi.org/10.1074/jbc.RA120.014643
citation_pmid	32816994
citation_public_url	https://hdl.handle.net/11094/78540

PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins

閲覧可能ファイル

論文情報

論文詳細を表示