Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation

Kim, Hyunsoo; Takegahara, Noriko; Walsh, Matthew C.; Ueda, Jun; Fujihara, Yoshitaka; Ikawa, Masahito; Choi, Yongwon

doi:https://doi.org/10.5483/BMBRep.2020.53.9.050

Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation

Kim, Hyunsoo; Takegahara, Noriko; Walsh, Matthew C. et al.

BMB Reports, 2020, 53(9), 472-477

アクセス数:255件（2025-04-18 03:03 集計）

固定URL: https://hdl.handle.net/11094/78541

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タイトル	Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation
著者	著者名 Kim, Hyunsoo
	著者名 Takegahara, Noriko
	著者名 Walsh, Matthew C.
	NRID 1000080450394 1000080450394 著者名 Ueda, Jun
	NRID 1000070578848 1000070578848 著者名 Fujihara, Yoshitaka
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	著者名 Choi, Yongwon
キーワード等	Bone homeostasis
	Maturation
	Multinucleation
	Osteoclast
	Pcdh7
内容	Kim, H., Takegahara, N., Walsh, M. C., Ueda, J., Fujihara, Y., Ikawa, M., & Choi, Y. (2020). Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation. BMB Reports, 53(9), 472-477. doi:10.5483/BMBRep.2020.53.9.050
抄録	Osteoclasts are hematopoietic-derived cells that resorb bone. They are required to maintain proper bone homeostasis and skeletal strength. Although osteoclast differentiation depends on receptor activator of NF-κB ligand (RANKL) stimulation, additional molecules further contribute to osteoclast maturation. Here, we demonstrate that protocadherin-7 (Pcdh7) regulates formation of multinucleated osteoclasts and contributes to maintenance of bone homeostasis. We found that Pcdh7 expression is induced by RANKL stimulation, and that RNAi-mediated knockdown of Pcdh7 resulted in impaired formation of osteoclasts. We generated Pcdh7-deficient mice and found increased bone mass due to decreased bone resorption but without any defect in bone formation. Using an in vitro culture system, it was revealed that formation of multinucleated osteoclasts is impaired in Pcdh7-deficient cultures, while no apparent defects were observed in differentiation and function of Pcdh7-deficient osteoblasts. Taken together, these results reveal an osteoclast cell-intrinsic role for Pcdh7 in maintaining bone homeostasis.
出版者	The Biochemical Society of the Republic of Korea
収録物名	BMB Reports
巻(号)	53(9)
ページ	472-477
刊行年月	2020-09-30
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78541
PISSN	19766696
eISSN	1976670X
NCID	AA1231107X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.5483/BMBRep.2020.53.9.050
関連情報 (isIdenticalTo)	PMID 32635982
アクセス権	open access
権利情報	© 2020 Korean Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	BMB Reports. 2020, 53(9), p. 472-477
DC.title	Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation
DC.creator	Kim, Hyunsoo
	Takegahara, Noriko
	Walsh, Matthew C.
	Ueda, Jun
	Fujihara, Yoshitaka
	Ikawa, Masahito
	Choi, Yongwon
DC.publisher	The Biochemical Society of the Republic of Korea
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-09-30
DC.identifier	https://doi.org/10.5483/BMBRep.2020.53.9.050
DC.subject	Bone homeostasis
	Maturation
	Multinucleation
	Osteoclast
	Pcdh7
DC.description	Kim, H., Takegahara, N., Walsh, M. C., Ueda, J., Fujihara, Y., Ikawa, M., & Choi, Y. (2020). Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation. BMB Reports, 53(9), 472-477. doi:10.5483/BMBRep.2020.53.9.050
DCTERMS.abstract	Osteoclasts are hematopoietic-derived cells that resorb bone. They are required to maintain proper bone homeostasis and skeletal strength. Although osteoclast differentiation depends on receptor activator of NF-κB ligand (RANKL) stimulation, additional molecules further contribute to osteoclast maturation. Here, we demonstrate that protocadherin-7 (Pcdh7) regulates formation of multinucleated osteoclasts and contributes to maintenance of bone homeostasis. We found that Pcdh7 expression is induced by RANKL stimulation, and that RNAi-mediated knockdown of Pcdh7 resulted in impaired formation of osteoclasts. We generated Pcdh7-deficient mice and found increased bone mass due to decreased bone resorption but without any defect in bone formation. Using an in vitro culture system, it was revealed that formation of multinucleated osteoclasts is impaired in Pcdh7-deficient cultures, while no apparent defects were observed in differentiation and function of Pcdh7-deficient osteoblasts. Taken together, these results reveal an osteoclast cell-intrinsic role for Pcdh7 in maintaining bone homeostasis.
DC.rights	© 2020 Korean Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	78541
citation_title	Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation
citation_author	Kim, Hyunsoo
	Takegahara, Noriko
	Walsh, Matthew C.
	Ueda, Jun
	Fujihara, Yoshitaka
	Ikawa, Masahito
	Choi, Yongwon
citation_publisher	The Biochemical Society of the Republic of Korea
citation_language	英語
citation_date	2020-09-30
citation_journal_title	BMB Reports
citation_volume	53
citation_issue	9
citation_firstpage	472
citation_lastpage	477
citation_doi	https://doi.org/10.5483/BMBRep.2020.53.9.050
citation_pmid	32635982
citation_public_url	https://hdl.handle.net/11094/78541

Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation

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