Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice

Noda, Taichi; Lu, Yonggang; Fujihara, Yoshitaka; Oura, Seiya; Koyano, Takayuki; Kobayashi, Sumire; Matzuk, Martin M.; Ikawa, Masahito

doi:https://doi.org/10.1073/pnas.1922650117

Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice

Noda, Taichi; Lu, Yonggang; Fujihara, Yoshitaka et al.

Proceedings of the National Academy of Sciences of the United States of America, 2020, 117(21), 11493-11502

アクセス数:242件（2025-04-18 02:44 集計）

固定URL: https://hdl.handle.net/11094/78546

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タイトル	Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice
著者	NRID 1000050712551 1000050712551 著者名 Noda, Taichi
	NRID 1000000817033 1000000817033 ORCID 0000-0003-0198-8906 0000-0003-0198-8906 researchmap permalink yonggang-lu yonggang-lu Scopus Author ID 57210966053 57210966053 著者名 Lu, Yonggang
	NRID 1000070578848 1000070578848 著者名 Fujihara, Yoshitaka
	著者名 Oura, Seiya
	著者名 Koyano, Takayuki
	著者名 Kobayashi, Sumire
	著者名 Matzuk, Martin M.
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
キーワード等	Infertility
	IZUMO1
	LLCFC1
	Sperm-oocyte fusion
	Transgenic
内容	Noda, T., Lu, Y., Fujihara, Y., Oura, S., Koyano, T., Kobayashi, S., . . . Ikawa, M. (2020). Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice. Proceedings of the National Academy of Sciences of the United States of America, 117(21) doi:10.1073/pnas.1922650117
抄録	Sperm-oocyte membrane fusion is one of the most important events for fertilization. So far, IZUMO1 and Fertilization Influencing Membrane Protein (FIMP) on the sperm membrane and CD9 and JUNO (IZUMO1R/FOLR4) on the oocyte membrane have been identified as fusion-required proteins. However, the molecular mechanisms for sperm-oocyte fusion are still unclear. Here, we show that testis-enriched genes, sperm-oocyte fusion required 1 (Sof1/Llcfc1/1700034O15Rik), transmembrane protein 95 (Tmem95), and sperm acrosome associated 6 (Spaca6), encode sperm proteins required for sperm-oocyte fusion in mice. These knockout (KO) spermatozoa carry IZUMO1 but cannot fuse with the oocyte plasma membrane, leading to male sterility. Transgenic mice which expressed mouse Sof1, Tmem95, and Spaca6 rescued the sterility of Sof1, Tmem95, and Spaca6 KO males, respectively. SOF1 and SPACA6 remain in acrosome-reacted spermatozoa, and SPACA6 translocates to the equatorial segment of these spermatozoa. The coexpression of SOF1, TMEM95, and SPACA6 in IZUMO1-expressing cultured cells did not enhance their ability to adhere to the oocyte membrane or allow them to fuse with oocytes. SOF1, TMEM95, and SPACA6 may function cooperatively with IZUMO1 and/or unknown fusogens in sperm-oocyte fusion.
出版者	National Academy of Sciences
収録物名	Proceedings of the National Academy of Sciences of the United States of America
巻(号)	117(21)
ページ	11493-11502
刊行年月	2020-05-26
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78546
PISSN	00278424
eISSN	10916490
NCID	AA11726874
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1073/pnas.1922650117
関連情報 (isIdenticalTo)	PMID 32393636
アクセス権	open access
権利情報	© 2020 the Author(s). Published by PNAS. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Proceedings of the National Academy of Sciences of the United States of America. 2020, 117(21), p. 11493-11502
DC.title	Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice
DC.creator	Noda, Taichi
	Lu, Yonggang
	Fujihara, Yoshitaka
	Oura, Seiya
	Koyano, Takayuki
	Kobayashi, Sumire
	Matzuk, Martin M.
	Ikawa, Masahito
DC.publisher	National Academy of Sciences
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-05-26
DC.identifier	https://doi.org/10.1073/pnas.1922650117
DC.subject	Infertility
	IZUMO1
	LLCFC1
	Sperm-oocyte fusion
	Transgenic
DC.description	Noda, T., Lu, Y., Fujihara, Y., Oura, S., Koyano, T., Kobayashi, S., . . . Ikawa, M. (2020). Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice. Proceedings of the National Academy of Sciences of the United States of America, 117(21) doi:10.1073/pnas.1922650117
DCTERMS.abstract	Sperm-oocyte membrane fusion is one of the most important events for fertilization. So far, IZUMO1 and Fertilization Influencing Membrane Protein (FIMP) on the sperm membrane and CD9 and JUNO (IZUMO1R/FOLR4) on the oocyte membrane have been identified as fusion-required proteins. However, the molecular mechanisms for sperm-oocyte fusion are still unclear. Here, we show that testis-enriched genes, sperm-oocyte fusion required 1 (Sof1/Llcfc1/1700034O15Rik), transmembrane protein 95 (Tmem95), and sperm acrosome associated 6 (Spaca6), encode sperm proteins required for sperm-oocyte fusion in mice. These knockout (KO) spermatozoa carry IZUMO1 but cannot fuse with the oocyte plasma membrane, leading to male sterility. Transgenic mice which expressed mouse Sof1, Tmem95, and Spaca6 rescued the sterility of Sof1, Tmem95, and Spaca6 KO males, respectively. SOF1 and SPACA6 remain in acrosome-reacted spermatozoa, and SPACA6 translocates to the equatorial segment of these spermatozoa. The coexpression of SOF1, TMEM95, and SPACA6 in IZUMO1-expressing cultured cells did not enhance their ability to adhere to the oocyte membrane or allow them to fuse with oocytes. SOF1, TMEM95, and SPACA6 may function cooperatively with IZUMO1 and/or unknown fusogens in sperm-oocyte fusion.
DC.rights	© 2020 the Author(s). Published by PNAS. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78546
citation_title	Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice
citation_author	Noda, Taichi
	Lu, Yonggang
	Fujihara, Yoshitaka
	Oura, Seiya
	Koyano, Takayuki
	Kobayashi, Sumire
	Matzuk, Martin M.
	Ikawa, Masahito
citation_publisher	National Academy of Sciences
citation_language	英語
citation_date	2020-05-26
citation_journal_title	Proceedings of the National Academy of Sciences of the United States of America
citation_volume	117
citation_issue	21
citation_firstpage	11493
citation_lastpage	11502
citation_doi	https://doi.org/10.1073/pnas.1922650117
citation_pmid	32393636
citation_public_url	https://hdl.handle.net/11094/78546

Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice

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