Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice

Fujihara, Yoshitaka; Lu, Yonggang; Noda, Taichi; Oji, Asami; Larasati, Tamara; Kojima, Kanako; Yu, Zhifeng; Matzuk, Ryan M.; Matzuk, Martin M.; Ikawa, Masahito

doi:https://doi.org/10.1073/pnas.1917060117

Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice

Fujihara, Yoshitaka; Lu, Yonggang; Noda, Taichi et al.

Proceedings of the National Academy of Sciences of the United States of America, 2020, 117(17), 9393-9400

アクセス数:283件（2025-04-18 03:08 集計）

固定URL: https://hdl.handle.net/11094/78547

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
ProcNatlAcadSciUSA_117_17_9393	pdf	なし	1.12 MB	160	2021.02.08

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice
著者	NRID 1000070578848 1000070578848 著者名 Fujihara, Yoshitaka
	NRID 1000000817033 1000000817033 ORCID 0000-0003-0198-8906 0000-0003-0198-8906 researchmap permalink yonggang-lu yonggang-lu Scopus Author ID 57210966053 57210966053 著者名 Lu, Yonggang
	NRID 1000050712551 1000050712551 著者名 Noda, Taichi
	NRID 1000050803417 1000050803417 著者名 Oji, Asami
	著者名 Larasati, Tamara
	NRID 1000060813631 1000060813631 researchmap permalink kojima-kita-kanako kojima-kita-kanako Scopus Author ID 36008685400 36008685400 著者名 Kojima, Kanako 著者の別表記 Kita, Kanako
	著者名 Yu, Zhifeng
	著者名 Matzuk, Ryan M.
	著者名 Matzuk, Martin M.
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
キーワード等	CRISPR/Cas9
	Fertilization
	Infertility
	IZUMO1
	Transgenic
内容	Fujihara, Y., Lu, Y., Noda, T., Oji, A., Larasati, T., Kojima-Kita, K., . . . Ikawa, M. (2020). Spermatozoa lacking fertilization influencing membrane protein (FIMP) fail to fuse with oocytes in mice. Proceedings of the National Academy of Sciences of the United States of America, 117(17), 9393-9400. doi:10.1073/pnas.1917060117
抄録	Sperm-oocyte fusion is a critical event in mammalian fertilization, categorized by three indispensable proteins. Sperm membrane protein IZUMO1 and its counterpart oocyte membrane protein JUNO make a protein complex allowing sperm to interact with the oocyte, and subsequent sperm-oocyte fusion. Oocyte tetraspanin protein CD9 also contributes to sperm-oocyte fusion. However, the fusion process cannot be explained solely by these three essential factors. In this study, we focused on analyzing a testis-specific gene 4930451I11Rik and generated mutant mice using the CRISPR/Cas9 system. Although IZUMO1 remained in 4930451I11Rik knockout (KO) spermatozoa, the KO spermatozoa were unable to fuse with oocytes and the KO males were severely subfertile. 4930451I11Rik encodes two isoforms: a transmembrane (TM) form and a secreted form. Both CRISPR/Cas9-mediated TM deletion and transgenic (Tg) rescue with the TM form revealed that only the TM form plays a critical role in sperm-oocyte fusion. Thus, we renamed this TM form Fertilization Influencing Membrane Protein (FIMP). The mCherry-tagged FIMP TM form was localized to the sperm equatorial segment where the sperm-oocyte fusion event occurs. Thus, FIMP is a sperm-specific transmembrane protein that is necessary for the sperm-oocyte fusion process.
出版者	National Academy of Sciences
収録物名	Proceedings of the National Academy of Sciences of the United States of America
巻(号)	117(17)
ページ	9393-9400
刊行年月	2020-04-28
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78547
PISSN	00278424
eISSN	10916490
NCID	AA11726874
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1073/pnas.1917060117
関連情報 (isIdenticalTo)	PMID 32295885
アクセス権	open access
権利情報	© 2020 the Author(s). Published by PNAS. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Proceedings of the National Academy of Sciences of the United States of America. 2020, 117(17), p. 9393-9400
DC.title	Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice
DC.creator	Fujihara, Yoshitaka
	Lu, Yonggang
	Noda, Taichi
	Oji, Asami
	Larasati, Tamara
	Kojima, Kanako
	Yu, Zhifeng
	Matzuk, Ryan M.
	Matzuk, Martin M.
	Ikawa, Masahito
DC.creator	Kita, Kanako
DC.publisher	National Academy of Sciences
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-04-28
DC.identifier	https://doi.org/10.1073/pnas.1917060117
DC.subject	CRISPR/Cas9
	Fertilization
	Infertility
	IZUMO1
	Transgenic
DC.description	Fujihara, Y., Lu, Y., Noda, T., Oji, A., Larasati, T., Kojima-Kita, K., . . . Ikawa, M. (2020). Spermatozoa lacking fertilization influencing membrane protein (FIMP) fail to fuse with oocytes in mice. Proceedings of the National Academy of Sciences of the United States of America, 117(17), 9393-9400. doi:10.1073/pnas.1917060117
DCTERMS.abstract	Sperm-oocyte fusion is a critical event in mammalian fertilization, categorized by three indispensable proteins. Sperm membrane protein IZUMO1 and its counterpart oocyte membrane protein JUNO make a protein complex allowing sperm to interact with the oocyte, and subsequent sperm-oocyte fusion. Oocyte tetraspanin protein CD9 also contributes to sperm-oocyte fusion. However, the fusion process cannot be explained solely by these three essential factors. In this study, we focused on analyzing a testis-specific gene 4930451I11Rik and generated mutant mice using the CRISPR/Cas9 system. Although IZUMO1 remained in 4930451I11Rik knockout (KO) spermatozoa, the KO spermatozoa were unable to fuse with oocytes and the KO males were severely subfertile. 4930451I11Rik encodes two isoforms: a transmembrane (TM) form and a secreted form. Both CRISPR/Cas9-mediated TM deletion and transgenic (Tg) rescue with the TM form revealed that only the TM form plays a critical role in sperm-oocyte fusion. Thus, we renamed this TM form Fertilization Influencing Membrane Protein (FIMP). The mCherry-tagged FIMP TM form was localized to the sperm equatorial segment where the sperm-oocyte fusion event occurs. Thus, FIMP is a sperm-specific transmembrane protein that is necessary for the sperm-oocyte fusion process.
DC.rights	© 2020 the Author(s). Published by PNAS. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78547
citation_title	Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice
citation_author	Fujihara, Yoshitaka
	Lu, Yonggang
	Noda, Taichi
	Oji, Asami
	Larasati, Tamara
	Kojima, Kanako
	Yu, Zhifeng
	Matzuk, Ryan M.
	Matzuk, Martin M.
	Ikawa, Masahito
citation_publisher	National Academy of Sciences
citation_language	英語
citation_date	2020-04-28
citation_journal_title	Proceedings of the National Academy of Sciences of the United States of America
citation_volume	117
citation_issue	17
citation_firstpage	9393
citation_lastpage	9400
citation_doi	https://doi.org/10.1073/pnas.1917060117
citation_pmid	32295885
citation_public_url	https://hdl.handle.net/11094/78547

Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice

閲覧可能ファイル

論文情報

論文詳細を表示