PITHD1 is a proteasome-interacting protein essential for male fertilization

Kondo, Hiroyuki; Matsumura, Takafumi; Kaneko, Mari; Inoue, Kenichi; Kosako, Hidetaka; Ikawa, Masahito; Takahama, Yousuke; Ohigashi, Izumi

doi:https://doi.org/10.1074/jbc.RA119.011144

PITHD1 is a proteasome-interacting protein essential for male fertilization

Kondo, Hiroyuki; Matsumura, Takafumi; Kaneko, Mari et al.

Journal of Biological Chemistry, 2020, 295(6), 1658-1672

アクセス数:249件（2025-04-18 02:32 集計）

固定URL: https://hdl.handle.net/11094/78549

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タイトル	PITHD1 is a proteasome-interacting protein essential for male fertilization
著者	著者名 Kondo, Hiroyuki
	著者名 Matsumura, Takafumi
	著者名 Kaneko, Mari
	著者名 Inoue, Kenichi
	NRID 1000010291171 1000010291171 著者名 Kosako, Hidetaka
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000020183858 1000020183858 著者名 Takahama, Yousuke
	NRID 1000000596588 1000000596588 著者名 Ohigashi, Izumi
内容	Hiroyuki Kondo, Takafumi Matsumura, Mari Kaneko, Kenichi Inoue, Hidetaka Kosako, Masahito Ikawa, Yousuke Takahama, Izumi Ohigashi, PITHD1 is a proteasome-interacting protein essential for male fertilization, Journal of Biological Chemistry, Volume 295, Issue 6, 2020, Pages 1658-1672, ISSN 0021-9258, https://doi.org/10.1074/jbc.RA119.011144.
抄録	The proteasome is a protein-degrading molecular complex that is necessary for protein homeostasis and various biological functions, including cell cycle regulation, signal transduction, and immune response. Proteasome activity is finely regulated by a variety of proteasome-interacting molecules. PITHD1 is a recently described molecule that has a domain putatively capable of interacting with the proteasome. However, it is unknown whether PITHD1 can actually bind to proteasomes and what it does in vivo. Here we report that PITHD1 is detected specifically in the spermatids in the testis and the cortical thymic epithelium in the thymus. Interestingly, PITHD1 associates with immunoproteasomes in the testis, but not with thymoproteasomes in the thymus. Mice deficient in PITHD1 exhibit severe male infertility accompanied with morphological abnormalities and impaired motility of spermatozoa. Furthermore, PITHD1 deficiency reduces proteasome activity in the testis and alters the amount of proteins that are important for fertilization capability by the sperm. However, the PITHD1-deficient mice demonstrate no detectable defects in the thymus, including T cell development. Collectively, our results identifyPITHD1as a proteasome- interacting protein that plays a nonredundant role in the male reproductive system.
出版者	American Society for Biochemistry and Molecular Biology Inc.
収録物名	Journal of Biological Chemistry
巻(号)	295(6)
ページ	1658-1672
刊行年月	2020-02-07
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78549
PISSN	00219258
eISSN	1083351X
NCID	AA1202441X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1074/jbc.RA119.011144
関連情報 (isIdenticalTo)	PMID 31915251
アクセス権	open access
権利情報	© 2020 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2020, 295(6), p. 1658-1672
DC.title	PITHD1 is a proteasome-interacting protein essential for male fertilization
DC.creator	Kondo, Hiroyuki
	Matsumura, Takafumi
	Kaneko, Mari
	Inoue, Kenichi
	Kosako, Hidetaka
	Ikawa, Masahito
	Takahama, Yousuke
	Ohigashi, Izumi
DC.publisher	American Society for Biochemistry and Molecular Biology Inc.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-02-07
DC.identifier	https://doi.org/10.1074/jbc.RA119.011144
DC.description	Hiroyuki Kondo, Takafumi Matsumura, Mari Kaneko, Kenichi Inoue, Hidetaka Kosako, Masahito Ikawa, Yousuke Takahama, Izumi Ohigashi, PITHD1 is a proteasome-interacting protein essential for male fertilization, Journal of Biological Chemistry, Volume 295, Issue 6, 2020, Pages 1658-1672, ISSN 0021-9258, https://doi.org/10.1074/jbc.RA119.011144.
DCTERMS.abstract	The proteasome is a protein-degrading molecular complex that is necessary for protein homeostasis and various biological functions, including cell cycle regulation, signal transduction, and immune response. Proteasome activity is finely regulated by a variety of proteasome-interacting molecules. PITHD1 is a recently described molecule that has a domain putatively capable of interacting with the proteasome. However, it is unknown whether PITHD1 can actually bind to proteasomes and what it does in vivo. Here we report that PITHD1 is detected specifically in the spermatids in the testis and the cortical thymic epithelium in the thymus. Interestingly, PITHD1 associates with immunoproteasomes in the testis, but not with thymoproteasomes in the thymus. Mice deficient in PITHD1 exhibit severe male infertility accompanied with morphological abnormalities and impaired motility of spermatozoa. Furthermore, PITHD1 deficiency reduces proteasome activity in the testis and alters the amount of proteins that are important for fertilization capability by the sperm. However, the PITHD1-deficient mice demonstrate no detectable defects in the thymus, including T cell development. Collectively, our results identifyPITHD1as a proteasome- interacting protein that plays a nonredundant role in the male reproductive system.
DC.rights	© 2020 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78549
citation_title	PITHD1 is a proteasome-interacting protein essential for male fertilization
citation_author	Kondo, Hiroyuki
	Matsumura, Takafumi
	Kaneko, Mari
	Inoue, Kenichi
	Kosako, Hidetaka
	Ikawa, Masahito
	Takahama, Yousuke
	Ohigashi, Izumi
citation_publisher	American Society for Biochemistry and Molecular Biology Inc.
citation_language	英語
citation_date	2020-02-07
citation_journal_title	Journal of Biological Chemistry
citation_volume	295
citation_issue	6
citation_firstpage	1658
citation_lastpage	1672
citation_doi	https://doi.org/10.1074/jbc.RA119.011144
citation_pmid	31915251
citation_public_url	https://hdl.handle.net/11094/78549

PITHD1 is a proteasome-interacting protein essential for male fertilization

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