Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis

Shimada, Keisuke; Kato, Hirotaka; Miyata, Haruhiko et al.

Journal of Reproduction and Development, 2019, 65(2), 155-162

アクセス数:187件（2025-04-18 02:54 集計）

固定URL: https://hdl.handle.net/11094/78560

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論文情報

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タイトル
Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis
著者
NRID
1000060779601 1000060779601
ORCID
0000-0003-3739-7163 0000-0003-3739-7163
researchmap permalink
nununununu nununununu
Scopus Author ID
56479808000 56479808000
著者名
Shimada, Keisuke
著者名
Kato, Hirotaka
NRID
1000050604732 1000050604732
researchmap permalink
hmiyata hmiyata
Scopus Author ID
37161837000 37161837000
ORCID
0000-0003-4758-5803 0000-0003-4758-5803
著者名
Miyata, Haruhiko
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
著者名
Ikawa, Masahito
キーワード等
Glycerol kinase
Male infertility
Mitochondrial sheath formation
Sperm mitochondria
Spermatogenesis
内容
Keisuke SHIMADA, Hirotaka KATO, Haruhiko MIYATA, Masahito IKAWA, Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis, Journal of Reproduction and Development, 2019, Volume 65, Issue 2, Pages 155-162, Released April 12, 2019, [Advance publication] Released January 21, 2019, Online ISSN 1348-4400, Print ISSN 0916-8818, https://doi.org/10.1262/jrd.2018-136, https://www.jstage.jst.go.jp/article/jrd/65/2/65_2018-136/_article/-char/en
抄録
The mitochondrial sheath is composed of mitochondria that coil tightly around the midpiece of sperm flagellum. These mitochondria are recruited from the cytoplasm to the flagellum late in spermatogenesis. Initially, recruited mitochondria are spherical-shaped but then elongate laterally to become crescent-like in shape. Subsequently, crescent-like mitochondria elongate continuously to coil tightly around the flagellum. Recently, disorganization of the mitochondrial sheath was reported in Glycerol kinase 2 (Gk2) disrupted mice. To analyze the disorganization of the mitochondrial sheath further, we generated Gk2-deficient mice using the CRISPR/Cas9 system and observed sperm mitochondria in testis using a freeze-fracture method with scanning electron microscopy. Gk2-disrupted spermatids show abnormal localization of crescent-like mitochondria, in spite of the initial proper alignment of spherical mitochondria around the flagellum, which causes abnormal mitochondrial sheath formation leading to exposure of the outer dense fibers. These results indicate that GK2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis.
出版者
The Japanese Society of Animal Reproduction (JSAR)
収録物名
Journal of Reproduction and Development
巻(号)
65(2)
ページ
155-162
刊行年月
2019
言語
英語
ハンドルURL
https://hdl.handle.net/11094/78560
PISSN
09168818
eISSN
13484400
NCID
AA12018734
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1262/jrd.2018-136
PMID
30662012
アクセス権
open access
権利情報
© 2019 the Society for Reproduction and Development. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article