An azoospermic factor gene, Ddx3y and its paralog, Ddx3x are dispensable in germ cells for male fertility

Matsumura, Takafumi; Endo, Tsutomu; Isotani, Ayako et al.

Journal of Reproduction and Development, 2019, 65(2), 121-128

アクセス数:197件（2025-04-30 11:31 集計）

固定URL: https://hdl.handle.net/11094/78562

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タイトル
An azoospermic factor gene, Ddx3y and its paralog, Ddx3x are dispensable in germ cells for male fertility
著者
著者名
Matsumura, Takafumi
NRID
1000040813936 1000040813936
著者名
Endo, Tsutomu
NRID
1000020444523 1000020444523
著者名
Isotani, Ayako
著者名
Ogawa, Masaki
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
著者名
Ikawa, Masahito
キーワード等
Azoospermia factor region
Chimeric analysis
CRISPR/Cas9
Male infertility
Y chromosome
内容
Takafumi MATSUMURA, Tsutomu ENDO, Ayako ISOTANI, Masaki OGAWA, Masahito IKAWA, An azoospermic factor gene, Ddx3y and its paralog, Ddx3x are dispensable in germ cells for male fertility, Journal of Reproduction and Development, 2019, Volume 65, Issue 2, Pages 121-128, Released April 12, 2019, [Advance publication] Released January 07, 2019, Online ISSN 1348-4400, Print ISSN 0916-8818, https://doi.org/10.1262/jrd.2018-145, https://www.jstage.jst.go.jp/article/jrd/65/2/65_2018-145/_article/-char/en
抄録
About 10% of male infertile patients show abnormalities in spermatogenesis. The microdeletion of azoospermia factor a (AZFa) region of the Y chromosome is thought to be a cause of spermatogenic failure. However, candidate gene responsible for the spermatogenic failure in AZFa deleted patients has not been elucidated yet. Using mice, we explored the function of Ddx3y, a strong candidate gene in the Azfa region, and Ddx3x, a Ddx3y paralog on the X chromosome, in spermatogenesis. We first generated Ddx3y KO male mice using CRISPR/Cas9 and found that the Ddx3y KO male mice show normal spermatogenesis, produce morphologically normal spermatozoa, and sire healthy offspring. Because Ddx3x KO males were embryonic lethal, we next generated chimeric mice, which contain Ddx3x and Ddx3y double KO (dKO) germ cells, and found that the dKO germ cells can differentiate into spermatozoa and transmit their mutant alleles to offspring by normal mating. We conclude that Ddx3x and Ddx3y are dispensable for spermatogenesis at least in mice. Unlike human, mice have an additional Ddx3y paralog D1pas1, that has been reported to be essential for spermatogenesis. These findings suggest that human and mouse DDX3 related proteins have distinct differences in their functions.
出版者
The Japanese Society of Animal Reproduction (JSAR)
収録物名
Journal of Reproduction and Development
巻(号)
65(2)
ページ
121-128
刊行年月
2019
言語
英語
ハンドルURL
https://hdl.handle.net/11094/78562
PISSN
09168818
eISSN
13484400
NCID
AA12018734
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1262/jrd.2018-145
PMID
30613052
アクセス権
open access
権利情報
© 2019 the Society for Reproduction and Development. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article