Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice

Noda, T.; Sakurai, N.; Nozawa, K.; Kobayashi, S.; Devlin, D. J.; Matzuk, M. M.; Ikawa, M.

doi:https://doi.org/10.1111/andr.12621

Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice

Noda, T.; Sakurai, N.; Nozawa, K. et al.

Andrology, 2019, 7(5), 644-653

アクセス数:962件（2025-10-24 14:10 集計）

固定URL: https://hdl.handle.net/11094/78564

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
Andrology_07_05_644	pdf	なし	4.36 MB	184	2021.02.08

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice
著者	NRID 1000050712551 1000050712551 著者名 Noda, T.
	著者名 Sakurai, N.
	著者名 Nozawa, K.
	著者名 Kobayashi, S.
	著者名 Devlin, D. J.
	著者名 Matzuk, M. M.
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, M.
キーワード等	genetically modified mice
	genome editing
	sperm maturation
内容	Noda, T., Sakurai, N., Nozawa, K., Kobayashi, S., Devlin, D. J., Matzuk, M. M., & Ikawa, M. (2019). Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice. Andrology, 7(5), 644-653. doi:10.1111/andr.12621
抄録	Background: Spermatozoa become competent for fertilization during transit through the epididymis. As spermatozoa from the proximal caudal epididymis can fertilize eggs, proteins from the caput and corpus epididymis are required for sperm maturation. Objectives: Microarray analysis identified that more than 17,000 genes are expressed in the epididymis; however, few of these genes demonstrate expression restricted to the epididymis. To analyze epididymis-enriched gene function in vivo, we generated knockout (KO) mutations in nine genes that are abundantly expressed in the caput and corpus region of the epididymis. Materials and methods: KO mice were generated using the CRISPR/Cas9 system. The histology of the epididymis was observed with hematoxylin and eosin staining. KO males were caged with wild-type females for 3–6 months to check fertility. Results: We generated individual mutant mouse lines having indel mutations in Pate1, Pate2, or Pate3. We also deleted the coding regions of Clpsl2, Epp13, and Rnase13, independently. Finally, the 150 kb region encoding Gm1110, Glb1l2, and Glb1l3 was deleted to generate a triple KO mouse line. Histology of the epididymis and sperm morphology of all KO lines were comparable to control males. The females mated with these KO males delivered pups at comparable numbers as control males. Discussion and conclusion: We revealed that nine genes abundantly expressed in the caput and corpus epididymis are dispensable for sperm function and male fecundity. CRISPR/Cas9-mediated KO mice generation accelerates the screening of epididymis-enriched genes for potential functions in reproduction.
出版者	Blackwell Publishing Ltd
収録物名	Andrology
巻(号)	7(5)
ページ	644-653
刊行年月	2019-08-09
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78564
PISSN	20472919
eISSN	20472927
NCID	AA12787207
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1111/andr.12621
関連情報 (isIdenticalTo)	PMID 30927342
アクセス権	open access
権利情報	© 2019 The Authors. Andrology published by John Wiley & Sons Ltd on behalf of American Society of Andrology and European Academy of Andrology. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Andrology. 2019, 7(5), p. 644-653
DC.title	Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice
DC.creator	Noda, T.
	Sakurai, N.
	Nozawa, K.
	Kobayashi, S.
	Devlin, D. J.
	Matzuk, M. M.
	Ikawa, M.
DC.publisher	Blackwell Publishing Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2019-08-09
DC.identifier	https://doi.org/10.1111/andr.12621
DC.subject	genetically modified mice
	genome editing
	sperm maturation
DC.description	Noda, T., Sakurai, N., Nozawa, K., Kobayashi, S., Devlin, D. J., Matzuk, M. M., & Ikawa, M. (2019). Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice. Andrology, 7(5), 644-653. doi:10.1111/andr.12621
DCTERMS.abstract	Background: Spermatozoa become competent for fertilization during transit through the epididymis. As spermatozoa from the proximal caudal epididymis can fertilize eggs, proteins from the caput and corpus epididymis are required for sperm maturation. Objectives: Microarray analysis identified that more than 17,000 genes are expressed in the epididymis; however, few of these genes demonstrate expression restricted to the epididymis. To analyze epididymis-enriched gene function in vivo, we generated knockout (KO) mutations in nine genes that are abundantly expressed in the caput and corpus region of the epididymis. Materials and methods: KO mice were generated using the CRISPR/Cas9 system. The histology of the epididymis was observed with hematoxylin and eosin staining. KO males were caged with wild-type females for 3–6 months to check fertility. Results: We generated individual mutant mouse lines having indel mutations in Pate1, Pate2, or Pate3. We also deleted the coding regions of Clpsl2, Epp13, and Rnase13, independently. Finally, the 150 kb region encoding Gm1110, Glb1l2, and Glb1l3 was deleted to generate a triple KO mouse line. Histology of the epididymis and sperm morphology of all KO lines were comparable to control males. The females mated with these KO males delivered pups at comparable numbers as control males. Discussion and conclusion: We revealed that nine genes abundantly expressed in the caput and corpus epididymis are dispensable for sperm function and male fecundity. CRISPR/Cas9-mediated KO mice generation accelerates the screening of epididymis-enriched genes for potential functions in reproduction.
DC.rights	© 2019 The Authors. Andrology published by John Wiley & Sons Ltd on behalf of American Society of Andrology and European Academy of Andrology. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78564
citation_title	Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice
citation_author	Noda, T.
	Sakurai, N.
	Nozawa, K.
	Kobayashi, S.
	Devlin, D. J.
	Matzuk, M. M.
	Ikawa, M.
citation_publisher	Blackwell Publishing Ltd
citation_language	英語
citation_date	2019-08-09
citation_journal_title	Andrology
citation_volume	7
citation_issue	5
citation_firstpage	644
citation_lastpage	653
citation_doi	https://doi.org/10.1111/andr.12621
citation_pmid	30927342
citation_public_url	https://hdl.handle.net/11094/78564

Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice

閲覧可能ファイル

論文情報

論文詳細を表示