Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice

Nozawa, Kaori; Satouh, Yuhkoh; Fujimoto, Takao; Oji, Asami; Ikawa, Masahito

doi:https://doi.org/10.1038/s41598-018-19497-6

Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice

Nozawa, Kaori; Satouh, Yuhkoh; Fujimoto, Takao et al.

Scientific Reports, 2018, 8(1), 1315

アクセス数:188件（2025-04-18 02:35 集計）

固定URL: https://hdl.handle.net/11094/78566

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タイトル	Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice
著者	著者名 Nozawa, Kaori
	NRID 1000040545571 1000040545571 著者名 Satouh, Yuhkoh
	著者名 Fujimoto, Takao
	NRID 1000050803417 1000050803417 著者名 Oji, Asami
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
内容	Nozawa, K., Satouh, Y., Fujimoto, T. et al. Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice. Sci Rep 8, 1315 (2018). https://doi.org/10.1038/s41598-018-19497-6
抄録	Sperm entry in mammalian oocytes triggers intracellular Ca2+ oscillations that initiate resumption of the meiotic cell cycle and subsequent activations. Here, we show that phospholipase C zeta 1 (PLCζ1) is the long-sought sperm-borne oocyte activation factor (SOAF). Plcz1 gene knockout (KO) mouse spermatozoa fail to induce Ca2+ changes in intracytoplasmic sperm injection (ICSI). In contrast to ICSI, Plcz1 KO spermatozoa induced atypical patterns of Ca2+ changes in normal fertilizations, and most of the fertilized oocytes ceased development at the 1-2-cell stage because of oocyte activation failure or polyspermy. We further discovered that both zona pellucida block to polyspermy (ZPBP) and plasma membrane block to polyspermy (PMBP) were delayed in oocytes fertilized with Plcz1 KO spermatozoa. With the observation that polyspermy is rare in astacin-like metalloendopeptidase (Astl) KO female oocytes that lack ZPBP, we conclude that PMPB plays more critical role than ZPBP in vivo. Finally, we obtained healthy pups from male mice carrying human infertile PLCZ1 mutation by single sperm ICSI supplemented with Plcz1 mRNA injection. These results suggest that mammalian spermatozoa have a primitive oocyte activation mechanism and that PLCζ1 is a SOAF that ensures oocyte activation steps for monospermic fertilization in mammals.
出版者	Nature Research
収録物名	Scientific Reports
巻(号)	8(1)
ページ	1315
刊行年月	2018-01-22
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78566
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-018-19497-6
関連情報 (isIdenticalTo)	PMID 29358633
アクセス権	open access
権利情報	© 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Scientific Reports. 2018, 8(1), p. 1315
DC.title	Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice
DC.creator	Nozawa, Kaori
	Satouh, Yuhkoh
	Fujimoto, Takao
	Oji, Asami
	Ikawa, Masahito
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-01-22
DC.identifier	https://doi.org/10.1038/s41598-018-19497-6
DC.description	Nozawa, K., Satouh, Y., Fujimoto, T. et al. Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice. Sci Rep 8, 1315 (2018). https://doi.org/10.1038/s41598-018-19497-6
DCTERMS.abstract	Sperm entry in mammalian oocytes triggers intracellular Ca2+ oscillations that initiate resumption of the meiotic cell cycle and subsequent activations. Here, we show that phospholipase C zeta 1 (PLCζ1) is the long-sought sperm-borne oocyte activation factor (SOAF). Plcz1 gene knockout (KO) mouse spermatozoa fail to induce Ca2+ changes in intracytoplasmic sperm injection (ICSI). In contrast to ICSI, Plcz1 KO spermatozoa induced atypical patterns of Ca2+ changes in normal fertilizations, and most of the fertilized oocytes ceased development at the 1-2-cell stage because of oocyte activation failure or polyspermy. We further discovered that both zona pellucida block to polyspermy (ZPBP) and plasma membrane block to polyspermy (PMBP) were delayed in oocytes fertilized with Plcz1 KO spermatozoa. With the observation that polyspermy is rare in astacin-like metalloendopeptidase (Astl) KO female oocytes that lack ZPBP, we conclude that PMPB plays more critical role than ZPBP in vivo. Finally, we obtained healthy pups from male mice carrying human infertile PLCZ1 mutation by single sperm ICSI supplemented with Plcz1 mRNA injection. These results suggest that mammalian spermatozoa have a primitive oocyte activation mechanism and that PLCζ1 is a SOAF that ensures oocyte activation steps for monospermic fertilization in mammals.
DC.rights	© 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78566
citation_title	Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice
citation_author	Nozawa, Kaori
	Satouh, Yuhkoh
	Fujimoto, Takao
	Oji, Asami
	Ikawa, Masahito
citation_publisher	Nature Research
citation_language	英語
citation_date	2018-01-22
citation_journal_title	Scientific Reports
citation_volume	8
citation_issue	1
citation_firstpage	1315
citation_doi	https://doi.org/10.1038/s41598-018-19497-6
citation_pmid	29358633
citation_public_url	https://hdl.handle.net/11094/78566

Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice

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