Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice

Fujihara, Yoshitaka; Oji, Asami; Kojima, Kanako; Larasati, Tamara; Ikawa, Masahito

doi:https://doi.org/10.1242/jcs.221481

Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice

Fujihara, Yoshitaka; Oji, Asami; Kojima, Kanako et al.

Journal of Cell Science, 2018, 131(19), jcs.221481

アクセス数:193件（2025-04-18 02:54 集計）

固定URL: https://hdl.handle.net/11094/78568

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タイトル	Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice
著者	NRID 1000070578848 1000070578848 著者名 Fujihara, Yoshitaka
	NRID 1000050803417 1000050803417 著者名 Oji, Asami
	NRID 1000060813631 1000060813631 researchmap permalink kojima-kita-kanako kojima-kita-kanako Scopus Author ID 36008685400 36008685400 著者名 Kojima, Kanako 著者の別表記 Kita, Kanako
	著者名 Larasati, Tamara
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
キーワード等	CKLF
	CMTM gene cluster
	CRISPR/Cas9
	Fertilization
	Testis
	Zona pellucida
抄録	Chemokines are signaling proteins that are secreted to induce chemotaxis during an immunological response. However, the functions of transmembrane-type chemokine-like factor (CKLF) and the CMTM (CKLF-like MARVEL transmembrane domain containing) protein family remain to be determined. In this study, we focused on the testis-specific mouse CMTM gene cluster (Cmtm1, Cmtm2a and Cmtm2b) and generated CRISPR/Cas9-mediated mutant mice to examine their physiological functions. Although Cmtm1 mutant mice were fertile, Cmtm2a and Cmtm2b double mutant mice had defects in male fertility due to impaired sperm function. We found that co-expression of sperm membrane proteins CMTM2A and CMTM2B is required for male fertility and affects the localization of the sperm membrane protein ADAM3 in regulating sperm fertilizing ability.
出版者	Company of Biologists Ltd
収録物名	Journal of Cell Science
巻(号)	131(19)
ページ	jcs.221481
刊行年月	2018-10-08
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78568
PISSN	00219533
eISSN	14779137
NCID	AA11720335
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1242/jcs.221481
関連情報 (isIdenticalTo)	PMID 30209135
アクセス権	open access
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Cell Science. 2018, 131(19), p. jcs.221481
DC.title	Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice
DC.creator	Fujihara, Yoshitaka
	Oji, Asami
	Kojima, Kanako
	Larasati, Tamara
	Ikawa, Masahito
DC.creator	Kita, Kanako
DC.publisher	Company of Biologists Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-10-08
DC.identifier	https://doi.org/10.1242/jcs.221481
DC.subject	CKLF
	CMTM gene cluster
	CRISPR/Cas9
	Fertilization
	Testis
	Zona pellucida
DCTERMS.abstract	Chemokines are signaling proteins that are secreted to induce chemotaxis during an immunological response. However, the functions of transmembrane-type chemokine-like factor (CKLF) and the CMTM (CKLF-like MARVEL transmembrane domain containing) protein family remain to be determined. In this study, we focused on the testis-specific mouse CMTM gene cluster (Cmtm1, Cmtm2a and Cmtm2b) and generated CRISPR/Cas9-mediated mutant mice to examine their physiological functions. Although Cmtm1 mutant mice were fertile, Cmtm2a and Cmtm2b double mutant mice had defects in male fertility due to impaired sperm function. We found that co-expression of sperm membrane proteins CMTM2A and CMTM2B is required for male fertility and affects the localization of the sperm membrane protein ADAM3 in regulating sperm fertilizing ability.
DC.format	78568
citation_title	Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice
citation_author	Fujihara, Yoshitaka
	Oji, Asami
	Kojima, Kanako
	Larasati, Tamara
	Ikawa, Masahito
citation_publisher	Company of Biologists Ltd
citation_language	英語
citation_date	2018-10-08
citation_journal_title	Journal of Cell Science
citation_volume	131
citation_issue	19
citation_firstpage	jcs.221481
citation_doi	https://doi.org/10.1242/jcs.221481
citation_pmid	30209135
citation_public_url	https://hdl.handle.net/11094/78568

Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice

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