mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility

Sakamoto, Satoko; Thumkeo, Dean; Ohta, Hiroshi; Zhang, Zhen; Huang, Shuangru; Kanchanawong, Pakorn; Fuu, Takayoshi; Watanabe, Sadanori; Shimada, Kentaro; Fujihara, Yoshitaka; Yoshida, Shosei; Ikawa, Masahito; Watanabe, Naoki; Saitou, Mitinori; Narumiya, Shuh

doi:https://doi.org/10.1371/journal.pbio.2004874

mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility

Sakamoto, Satoko; Thumkeo, Dean; Ohta, Hiroshi et al.

PLoS Biology, 2018, 16(9), e2004874

アクセス数:258件（2025-04-18 02:00 集計）

固定URL: https://hdl.handle.net/11094/78570

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タイトル	mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility
著者	NRID 1000070648427 1000070648427 著者名 Sakamoto, Satoko
	NRID 1000040372594 1000040372594 著者名 Thumkeo, Dean
	NRID 1000050391892 1000050391892 著者名 Ohta, Hiroshi
	著者名 Zhang, Zhen
	著者名 Huang, Shuangru
	著者名 Kanchanawong, Pakorn
	著者名 Fuu, Takayoshi
	著者名 Watanabe, Sadanori
	著者名 Shimada, Kentaro
	NRID 1000070578848 1000070578848 著者名 Fujihara, Yoshitaka
	NRID 1000060294138 1000060294138 著者名 Yoshida, Shosei
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000080303816 1000080303816 著者名 Watanabe, Naoki
	NRID 1000080373306 1000080373306 著者名 Saitou, Mitinori
	NRID 1000070144350 1000070144350 著者名 Narumiya, Shuh
内容	mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility. Sakamoto S, Thumkeo D, Ohta H, Zhang Z, Huang S, et al. PLOS Biology. 2018. 16(9) : e2004874. doi:10.1371/journal.pbio.2004874
抄録	Formin is one of the two major classes of actin binding proteins (ABPs) with nucleation and polymerization activity. However, despite advances in our understanding of its biochemical activity, whether and how formins generate specific architecture of the actin cytoskeleton and function in a physiological context in vivo remain largely obscure. It is also unknown how actin filaments generated by formins interact with other ABPs in the cell. Here, we combine genetic manipulation of formins mammalian diaphanous homolog1 (mDia1) and 3 (mDia3) with superresolution microscopy and single-molecule imaging, and show that the formins mDia1 and mDia3 are dominantly expressed in Sertoli cells of mouse seminiferous tubule and together generate a highly dynamic cortical filamentous actin (F-actin) meshwork that is continuous with the contractile actomyosin bundles. Loss of mDia1/3 impaired these F-actin architectures, induced ectopic noncontractile espin1-containing F-actin bundles, and disrupted Sertoli cell–germ cell interaction, resulting in impaired spermatogenesis. These results together demonstrate the previously unsuspected mDia-dependent regulatory mechanism of cortical F-actin that is indispensable for mammalian sperm development and male fertility.
出版者	Public Library of Science
収録物名	PLoS Biology
巻(号)	16(9)
ページ	e2004874
刊行年月	2018-09-26
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78570
PISSN	15449173
eISSN	15457885
NCID	AA11886145
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1371/journal.pbio.2004874
関連情報 (isIdenticalTo)	PMID 30256801
アクセス権	open access
権利情報	© 2018 Sakamoto et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	PLoS Biology. 2018, 16(9), p. e2004874
DC.title	mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility
DC.creator	Sakamoto, Satoko
	Thumkeo, Dean
	Ohta, Hiroshi
	Zhang, Zhen
	Huang, Shuangru
	Kanchanawong, Pakorn
	Fuu, Takayoshi
	Watanabe, Sadanori
	Shimada, Kentaro
	Fujihara, Yoshitaka
	Yoshida, Shosei
	Ikawa, Masahito
	Watanabe, Naoki
	Saitou, Mitinori
	Narumiya, Shuh
DC.publisher	Public Library of Science
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-09-26
DC.identifier	https://doi.org/10.1371/journal.pbio.2004874
DC.description	mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility. Sakamoto S, Thumkeo D, Ohta H, Zhang Z, Huang S, et al. PLOS Biology. 2018. 16(9) : e2004874. doi:10.1371/journal.pbio.2004874
DCTERMS.abstract	Formin is one of the two major classes of actin binding proteins (ABPs) with nucleation and polymerization activity. However, despite advances in our understanding of its biochemical activity, whether and how formins generate specific architecture of the actin cytoskeleton and function in a physiological context in vivo remain largely obscure. It is also unknown how actin filaments generated by formins interact with other ABPs in the cell. Here, we combine genetic manipulation of formins mammalian diaphanous homolog1 (mDia1) and 3 (mDia3) with superresolution microscopy and single-molecule imaging, and show that the formins mDia1 and mDia3 are dominantly expressed in Sertoli cells of mouse seminiferous tubule and together generate a highly dynamic cortical filamentous actin (F-actin) meshwork that is continuous with the contractile actomyosin bundles. Loss of mDia1/3 impaired these F-actin architectures, induced ectopic noncontractile espin1-containing F-actin bundles, and disrupted Sertoli cell–germ cell interaction, resulting in impaired spermatogenesis. These results together demonstrate the previously unsuspected mDia-dependent regulatory mechanism of cortical F-actin that is indispensable for mammalian sperm development and male fertility.
DC.rights	© 2018 Sakamoto et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78570
citation_title	mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility
citation_author	Sakamoto, Satoko
	Thumkeo, Dean
	Ohta, Hiroshi
	Zhang, Zhen
	Huang, Shuangru
	Kanchanawong, Pakorn
	Fuu, Takayoshi
	Watanabe, Sadanori
	Shimada, Kentaro
	Fujihara, Yoshitaka
	Yoshida, Shosei
	Ikawa, Masahito
	Watanabe, Naoki
	Saitou, Mitinori
	Narumiya, Shuh
citation_publisher	Public Library of Science
citation_language	英語
citation_date	2018-09-26
citation_journal_title	PLoS Biology
citation_volume	16
citation_issue	9
citation_firstpage	e2004874
citation_doi	https://doi.org/10.1371/journal.pbio.2004874
citation_pmid	30256801
citation_public_url	https://hdl.handle.net/11094/78570

mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility

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