Vestigial-like 2 contributes to normal muscle fiber type distribution in mice

Honda, Masahiko; Hidaka, Kyoko; Fukada, So-ichiro; Sugawa, Ryo; Shirai, Manabu; Ikawa, Masahito; Morisaki, Takayuki

doi:https://doi.org/10.1038/s41598-017-07149-0

Vestigial-like 2 contributes to normal muscle fiber type distribution in mice

Honda, Masahiko; Hidaka, Kyoko; Fukada, So-ichiro et al.

Scientific Reports, 2017, 7(1), 7168

アクセス数:386件（2025-04-29 13:09 集計）

固定URL: https://hdl.handle.net/11094/78576

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タイトル	Vestigial-like 2 contributes to normal muscle fiber type distribution in mice
著者	NRID 1000010455545 1000010455545 著者名 Honda, Masahiko
	NRID 1000000216681 1000000216681 著者名 Hidaka, Kyoko
	NRID 1000020432445 1000020432445 ORCID 0000-0003-4051-5108 0000-0003-4051-5108 researchmap permalink mdx mdx Scopus Author ID 56020906000 56020906000 著者名 Fukada, So-ichiro
	著者名 Sugawa, Ryo
	NRID 1000070294121 1000070294121 著者名 Shirai, Manabu
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000030174410 1000030174410 著者名 Morisaki, Takayuki
内容	Honda, M., Hidaka, K., Fukada, Si. et al. Vestigial-like 2 contributes to normal muscle fiber type distribution in mice. Sci Rep 7, 7168 (2017). https://doi.org/10.1038/s41598-017-07149-0
抄録	Skeletal muscle is composed of heterogeneous populations of myofibers that are classified as slow- and fast-twitch fibers. The muscle fiber-type is regulated in a coordinated fashion by multiple genes, including transcriptional factors and microRNAs (miRNAs). However, players involved in this regulation are not fully elucidated. One of the members of the Vestigial-like factors, Vgll2, is thought to play a pivotal role in TEA domain (TEAD) transcription factor-mediated muscle-specific gene expression because of its restricted expression in skeletal muscles of adult mice. Here, we generated Vgll2 null mice and investigated Vgll2 function in adult skeletal muscles. These mice presented an increased number of fast-twitch type IIb fibers and exhibited a down-regulation of slow type I myosin heavy chain (MyHC) gene, Myh7, which resulted in exercise intolerance. In accordance with the decrease in Myh7, down-regulation of miR-208b, encoded within Myh7 gene and up-regulation of targets of miR-208b, Sox6, Sp3, and Purβ, were observed in Vgll2 deficient mice. Moreover, we detected the physical interaction between Vgll2 and TEAD1/4 in neonatal skeletal muscles. These results suggest that Vgll2 may be both directly and indirectly involved in the programing of slow muscle fibers through the formation of the Vgll2-TEAD complex.
出版者	Nature Research
収録物名	Scientific Reports
巻(号)	7(1)
ページ	7168
刊行年月	2017-08-02
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78576
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-017-07149-0
関連情報 (isIdenticalTo)	PMID 28769032
アクセス権	open access
権利情報	© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

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資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Scientific Reports. 2017, 7(1), p. 7168
DC.title	Vestigial-like 2 contributes to normal muscle fiber type distribution in mice
DC.creator	Honda, Masahiko
	Hidaka, Kyoko
	Fukada, So-ichiro
	Sugawa, Ryo
	Shirai, Manabu
	Ikawa, Masahito
	Morisaki, Takayuki
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2017-08-02
DC.identifier	https://doi.org/10.1038/s41598-017-07149-0
DC.description	Honda, M., Hidaka, K., Fukada, Si. et al. Vestigial-like 2 contributes to normal muscle fiber type distribution in mice. Sci Rep 7, 7168 (2017). https://doi.org/10.1038/s41598-017-07149-0
DCTERMS.abstract	Skeletal muscle is composed of heterogeneous populations of myofibers that are classified as slow- and fast-twitch fibers. The muscle fiber-type is regulated in a coordinated fashion by multiple genes, including transcriptional factors and microRNAs (miRNAs). However, players involved in this regulation are not fully elucidated. One of the members of the Vestigial-like factors, Vgll2, is thought to play a pivotal role in TEA domain (TEAD) transcription factor-mediated muscle-specific gene expression because of its restricted expression in skeletal muscles of adult mice. Here, we generated Vgll2 null mice and investigated Vgll2 function in adult skeletal muscles. These mice presented an increased number of fast-twitch type IIb fibers and exhibited a down-regulation of slow type I myosin heavy chain (MyHC) gene, Myh7, which resulted in exercise intolerance. In accordance with the decrease in Myh7, down-regulation of miR-208b, encoded within Myh7 gene and up-regulation of targets of miR-208b, Sox6, Sp3, and Purβ, were observed in Vgll2 deficient mice. Moreover, we detected the physical interaction between Vgll2 and TEAD1/4 in neonatal skeletal muscles. These results suggest that Vgll2 may be both directly and indirectly involved in the programing of slow muscle fibers through the formation of the Vgll2-TEAD complex.
DC.rights	© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78576
citation_title	Vestigial-like 2 contributes to normal muscle fiber type distribution in mice
citation_author	Honda, Masahiko
	Hidaka, Kyoko
	Fukada, So-ichiro
	Sugawa, Ryo
	Shirai, Manabu
	Ikawa, Masahito
	Morisaki, Takayuki
citation_publisher	Nature Research
citation_language	英語
citation_date	2017-08-02
citation_journal_title	Scientific Reports
citation_volume	7
citation_issue	1
citation_firstpage	7168
citation_doi	https://doi.org/10.1038/s41598-017-07149-0
citation_pmid	28769032
citation_public_url	https://hdl.handle.net/11094/78576