Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

Ueda, Jun; Harada, Akihito; Urahama, Takashi; Machida, Shinichi; Maehara, Kazumitsu; Hada, Masashi; Makino, Yoshinori; Nogami, Jumpei; Horikoshi, Naoki; Osakabe, Akihisa; Taguchi, Hiroyuki; Tanaka, Hiroki; Tachiwana, Hiroaki; Yao, Tatsuma; Yamada, Minami; Iwamoto, Takashi; Isotani, Ayako; Ikawa, Masahito; Tachibana, Taro; Okada, Yuki; Kimura, Hiroshi; Ohkawa, Yasuyuki; Kurumizaka, Hitoshi; Yamagata, Kazuo

doi:https://doi.org/10.1016/j.celrep.2016.12.065

Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

Ueda, Jun; Harada, Akihito; Urahama, Takashi et al.

Cell Reports, 2017, 18(3), 593-600

アクセス数:741件（2025-04-18 02:17 集計）

固定URL: https://hdl.handle.net/11094/78582

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
CellRep_18_03_593	pdf	なし	4.16 MB	150	2021.02.08

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis
著者	NRID 1000080450394 1000080450394 著者名 Ueda, Jun
	NRID 1000060596823 1000060596823 著者名 Harada, Akihito
	著者名 Urahama, Takashi
	NRID 1000050732195 1000050732195 著者名 Machida, Shinichi
	NRID 1000090726431 1000090726431 著者名 Maehara, Kazumitsu
	NRID 1000010802746 1000010802746 著者名 Hada, Masashi
	著者名 Makino, Yoshinori
	NRID 1000060801751 1000060801751 著者名 Nogami, Jumpei
	著者名 Horikoshi, Naoki
	NRID 1000070632107 1000070632107 著者名 Osakabe, Akihisa
	著者名 Taguchi, Hiroyuki
	NRID 1000090880104 1000090880104 著者名 Tanaka, Hiroki
	NRID 1000070546382 1000070546382 著者名 Tachiwana, Hiroaki
	著者名 Yao, Tatsuma
	著者名 Yamada, Minami
	NRID 1000060223426 1000060223426 著者名 Iwamoto, Takashi
	NRID 1000020444523 1000020444523 著者名 Isotani, Ayako
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000080311752 1000080311752 著者名 Tachibana, Taro
	NRID 1000060546430 1000060546430 著者名 Okada, Yuki
	NRID 1000030241392 1000030241392 著者名 Kimura, Hiroshi
	NRID 1000080448430 1000080448430 著者名 Ohkawa, Yasuyuki
	NRID 1000080300870 1000080300870 著者名 Kurumizaka, Hitoshi
	NRID 1000010361312 1000010361312 著者名 Yamagata, Kazuo
キーワード等	chromatin
	crystal structure
	epigenetics
	histone variant
	meiosis
	nucleosome
	spermatogenesis
	spermatogonial differentiation
	spermatozoa
	testes
内容	Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata, Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis, Cell Reports, Volume 18, Issue 3, 2017, Pages 593-600, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2016.12.065.
抄録	Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.
出版者	Elsevier B.V.
収録物名	Cell Reports
巻(号)	18(3)
ページ	593-600
刊行年月	2017-01-17
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78582
eISSN	22111247
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.celrep.2016.12.065
関連情報 (isIdenticalTo)	PMID 28099840
アクセス権	open access
権利情報	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Cell Reports. 2017, 18(3), p. 593-600
DC.title	Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis
DC.creator	Ueda, Jun
	Harada, Akihito
	Urahama, Takashi
	Machida, Shinichi
	Maehara, Kazumitsu
	Hada, Masashi
	Makino, Yoshinori
	Nogami, Jumpei
	Horikoshi, Naoki
	Osakabe, Akihisa
	Taguchi, Hiroyuki
	Tanaka, Hiroki
	Tachiwana, Hiroaki
	Yao, Tatsuma
	Yamada, Minami
	Iwamoto, Takashi
	Isotani, Ayako
	Ikawa, Masahito
	Tachibana, Taro
	Okada, Yuki
	Kimura, Hiroshi
	Ohkawa, Yasuyuki
	Kurumizaka, Hitoshi
	Yamagata, Kazuo
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2017-01-17
DC.identifier	https://doi.org/10.1016/j.celrep.2016.12.065
DC.subject	chromatin
	crystal structure
	epigenetics
	histone variant
	meiosis
	nucleosome
	spermatogenesis
	spermatogonial differentiation
	spermatozoa
	testes
DC.description	Jun Ueda, Akihito Harada, Takashi Urahama, Shinichi Machida, Kazumitsu Maehara, Masashi Hada, Yoshinori Makino, Jumpei Nogami, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroki Tanaka, Hiroaki Tachiwana, Tatsuma Yao, Minami Yamada, Takashi Iwamoto, Ayako Isotani, Masahito Ikawa, Taro Tachibana, Yuki Okada, Hiroshi Kimura, Yasuyuki Ohkawa, Hitoshi Kurumizaka, Kazuo Yamagata, Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis, Cell Reports, Volume 18, Issue 3, 2017, Pages 593-600, ISSN 2211-1247, https://doi.org/10.1016/j.celrep.2016.12.065.
DCTERMS.abstract	Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.
DC.rights	© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78582
citation_title	Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis
citation_author	Ueda, Jun
	Harada, Akihito
	Urahama, Takashi
	Machida, Shinichi
	Maehara, Kazumitsu
	Hada, Masashi
	Makino, Yoshinori
	Nogami, Jumpei
	Horikoshi, Naoki
	Osakabe, Akihisa
	Taguchi, Hiroyuki
	Tanaka, Hiroki
	Tachiwana, Hiroaki
	Yao, Tatsuma
	Yamada, Minami
	Iwamoto, Takashi
	Isotani, Ayako
	Ikawa, Masahito
	Tachibana, Taro
	Okada, Yuki
	Kimura, Hiroshi
	Ohkawa, Yasuyuki
	Kurumizaka, Hitoshi
	Yamagata, Kazuo
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2017-01-17
citation_journal_title	Cell Reports
citation_volume	18
citation_issue	3
citation_firstpage	593
citation_lastpage	600
citation_doi	https://doi.org/10.1016/j.celrep.2016.12.065
citation_pmid	28099840
citation_public_url	https://hdl.handle.net/11094/78582

Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis

閲覧可能ファイル

論文情報

論文詳細を表示