Mouse spermatozoa with higher fertilization rates have thinner nuclei

Mashiko, Daisuke; Ikawa, Masahito; Fujimoto, Koichi

doi:https://doi.org/10.7717/peerj.3913

Mouse spermatozoa with higher fertilization rates have thinner nuclei

Mashiko, Daisuke; Ikawa, Masahito; Fujimoto, Koichi

PeerJ, 2017, 2017(10), e3913

アクセス数:133件（2025-04-18 02:24 集計）

固定URL: https://hdl.handle.net/11094/78584

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タイトル	Mouse spermatozoa with higher fertilization rates have thinner nuclei
著者	著者名 Mashiko, Daisuke
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000060334306 1000060334306 ORCID 0000-0001-6473-7990 0000-0001-6473-7990 researchmap permalink fujimotokoichi fujimotokoichi Scopus Author ID 36093950300 36093950300 著者名 Fujimoto, Koichi
キーワード等	Elliptic Fourier analysis
	Image analysis
	Morphometry
	Sperm
	Sperm head shape
内容	Mashiko D, Ikawa M, Fujimoto K. 2017. Mouse spermatozoa with higher fertilization rates have thinner nuclei. PeerJ 5:e3913 https://doi.org/10.7717/peerj.3913
抄録	Background. Although spermatozoa with normal morphology are assumed to have uniform fertilization ability, recent data show that even normal spermatozoa have considerable variation in their head shape which is associated with differences in fertilization ability. Appropriate quantitative indicators for good sperm morphology, however, remain unidentified. Methods. Therefore, in an effort to identify such an indicator, we compared the nuclear contour of normal mouse spermatozoa by quantitative multivariate analysis using elliptic Fourier descriptors combined with principal component analysis. The spermatozoa were obtained from different strains and collection sites which have been shown to be associated with different fertilization abilities. Results. We found that the head was 5.7% thinner in spermatozoa from the B6D2F1 (BDF1) strain, known to have a higher fertilization rate, than in those from the C57BL/6N (B6N) strain, which has a lower fertilization rate. Moreover, zona-penetrated spermatozoa in the perivitelline space consistently had 5.4% thinner heads than those isolated from the epididymis before ejaculation. The aspect ratio, which represents the sperm head thinness, uniquely distinguished these sperm populations, confirming its validity as a morphological indicator. Discussion. Because aspect ratio has also been shown to characterize human spermatozoa, this unique morphometric indicator might be applicable to compare normal spermatozoa among multiple patients, which will greatly facilitate and enhance current reproductive technologies.
出版者	PeerJ Inc.
収録物名	PeerJ
巻(号)	2017(10)
ページ	e3913
刊行年月	2017-10-12
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78584
eISSN	21678359
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.7717/peerj.3913
アクセス権	open access
権利情報	© 2017 Mashiko et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	PeerJ. 2017, 2017(10), p. e3913
DC.title	Mouse spermatozoa with higher fertilization rates have thinner nuclei
DC.creator	Mashiko, Daisuke
	Ikawa, Masahito
	Fujimoto, Koichi
DC.publisher	PeerJ Inc.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2017-10-12
DC.identifier	https://doi.org/10.7717/peerj.3913
DC.subject	Elliptic Fourier analysis
	Image analysis
	Morphometry
	Sperm
	Sperm head shape
DC.description	Mashiko D, Ikawa M, Fujimoto K. 2017. Mouse spermatozoa with higher fertilization rates have thinner nuclei. PeerJ 5:e3913 https://doi.org/10.7717/peerj.3913
DCTERMS.abstract	Background. Although spermatozoa with normal morphology are assumed to have uniform fertilization ability, recent data show that even normal spermatozoa have considerable variation in their head shape which is associated with differences in fertilization ability. Appropriate quantitative indicators for good sperm morphology, however, remain unidentified. Methods. Therefore, in an effort to identify such an indicator, we compared the nuclear contour of normal mouse spermatozoa by quantitative multivariate analysis using elliptic Fourier descriptors combined with principal component analysis. The spermatozoa were obtained from different strains and collection sites which have been shown to be associated with different fertilization abilities. Results. We found that the head was 5.7% thinner in spermatozoa from the B6D2F1 (BDF1) strain, known to have a higher fertilization rate, than in those from the C57BL/6N (B6N) strain, which has a lower fertilization rate. Moreover, zona-penetrated spermatozoa in the perivitelline space consistently had 5.4% thinner heads than those isolated from the epididymis before ejaculation. The aspect ratio, which represents the sperm head thinness, uniquely distinguished these sperm populations, confirming its validity as a morphological indicator. Discussion. Because aspect ratio has also been shown to characterize human spermatozoa, this unique morphometric indicator might be applicable to compare normal spermatozoa among multiple patients, which will greatly facilitate and enhance current reproductive technologies.
DC.rights	© 2017 Mashiko et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78584
citation_title	Mouse spermatozoa with higher fertilization rates have thinner nuclei
citation_author	Mashiko, Daisuke
	Ikawa, Masahito
	Fujimoto, Koichi
citation_publisher	PeerJ Inc.
citation_language	英語
citation_date	2017-10-12
citation_journal_title	PeerJ
citation_volume	2017
citation_issue	10
citation_firstpage	e3913
citation_doi	https://doi.org/10.7717/peerj.3913
citation_public_url	https://hdl.handle.net/11094/78584

Mouse spermatozoa with higher fertilization rates have thinner nuclei

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