学術雑誌論文

学術雑誌論文

Nature Communications. 2016, 7, p. 12198

Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization

Nature Research

英語

2016-07-15

https://doi.org/10.1038/ncomms12198

Kato, K., Satouh, Y., Nishimasu, H. et al. Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization. Nat Commun 7, 12198 (2016). https://doi.org/10.1038/ncomms12198

Sperm-egg fusion is the critical step in mammalian fertilization, and requires the interaction between IZUMO1 on the sperm surface and JUNO (also known as folate receptor (FR) 4 or IZUMO1R) on the egg surface. Whereas other FRs bind and uptake folates, JUNO binds IZUMO1 and establishes the cell-cell adhesion. However, the mechanism of IZUMO1 recognition by JUNO has remained elusive. Here we report the crystal structure of mouse JUNO, at 2.3 Å resolution. A structural comparison of JUNO with the FRs revealed that JUNO and the FRs have similar overall structures, but JUNO lacks the folate-binding pocket, thereby explaining the inability of JUNO to bind folate. Further complementation of Juno knockout eggs with mutant Juno messenger RNAs revealed that the conserved, surface-exposed tryptophan residue of JUNO is required for sperm binding and fertilization. Our structure-based in vivo functional analyses provide a framework towards a mechanistic understanding of mammalian gamete recognition.

© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.

78586

Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization

Nature Research

英語

2016-07-15

Nature Communications

7

12198

https://doi.org/10.1038/ncomms12198

27416963

https://hdl.handle.net/11094/78586