Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis

Shimada, Midori; Goshima, Takahiro; Matsuo, Hiromi et al.

Nature Communications, 2016, 7, 12059

アクセス数:306件（2025-04-18 02:22 集計）

固定URL: https://hdl.handle.net/11094/78587

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タイトル
Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis
著者
NRID
1000060444981 1000060444981
著者名
Shimada, Midori
著者名
Goshima, Takahiro
著者名
Matsuo, Hiromi
NRID
1000040616322 1000040616322
著者名
Johmura, Yoshikazu
著者名
Haruta, Mayumi
著者名
Murata, Kazuhiro
NRID
1000010263310 1000010263310
著者名
Tanaka, Hiromitsu
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
著者名
Ikawa, Masahito
NRID
1000050280813 1000050280813
著者名
Nakanishi, Keiko
NRID
1000040217774 1000040217774
著者名
Nakanishi, Makoto
内容
Shimada, M., Goshima, T., Matsuo, H. et al. Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis. Nat Commun 7, 12059 (2016). https://doi.org/10.1038/ncomms12059
抄録
Proper deposition and activation of Aurora B at the centromere is critical for faithful chromosome segregation in mammals. However, the mechanistic basis for abrupt Aurora B kinase activation at the centromere has not yet been fully understood. We demonstrate here that Aurora B-mediated phosphorylation of histone H2AX at serine 121 (H2AX-pS121) promotes Aurora B autophosphorylation and is essential for proper chromosome segregation. Aurora B-mediated H2AX-pS121 is specifically detected at the centromere during mitosis. H2AX depletion results in a severe defect in activation and deposition of Aurora B at this locus. A phosphomimic mutant of H2AX at S121 interacts with activated Aurora B more efficiently than wild-type in vitro. Taken together, these results propose a model in which Aurora B-mediated H2AX-pS121 probably provide a platform for Aurora B autoactivation circuitry at centromeres and thus play a pivotal role in proper chromosome segregation.
出版者
Nature Research
収録物名
Nature Communications
巻(号)
7
ページ
12059
刊行年月
2016-07-08
言語
英語
ハンドルURL
https://hdl.handle.net/11094/78587
eISSN
20411723
NCID
AA12645905
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1038/ncomms12059
PMID
27389782
アクセス権
open access
権利情報
© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article