Generation of Hprt-disrupted rat through mouse← rat ES chimeras

Isotani, Ayako; Yamagata, Kazuo; Okabe, Masaru et al.

Scientific Reports, 2016, 6, 24215

アクセス数:156件（2025-04-18 02:47 集計）

固定URL: https://hdl.handle.net/11094/78589

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タイトル
Generation of Hprt-disrupted rat through mouse← rat ES chimeras
著者
NRID
1000020444523 1000020444523
著者名
Isotani, Ayako
NRID
1000010361312 1000010361312
著者名
Yamagata, Kazuo
NRID
1000030089875 1000030089875
著者名
Okabe, Masaru
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
著者名
Ikawa, Masahito
内容
Isotani, A., Yamagata, K., Okabe, M. et al. Generation of Hprt-disrupted rat through mouse←rat ES chimeras. Sci Rep 6, 24215 (2016). https://doi.org/10.1038/srep24215
抄録
We established rat embryonic stem (ES) cell lines from a double transgenic rat line which harbours CAG-GFP for ubiquitous expression of GFP in somatic cells and Acr3-EGFP for expression in sperm (green body and green sperm: GBGS rat). By injecting the GBGS rat ES cells into mouse blastocysts and transplanting them into pseudopregnant mice, rat spermatozoa were produced in mouse← rat ES chimeras. Rat spermatozoa from the chimeric testis were able to fertilize eggs by testicular sperm extraction combined with intracytoplasmic sperm injection (TESE-ICSI). In the present paper, we disrupted rat hypoxanthine-guanine phosphoribosyl transferase (Hprt) gene in ES cells and produced a Hprt-disrupted rat line using the mouse← rat ES chimera system. The mouse← rat ES chimera system demonstrated the dual advantages of space conservation and a clear indication of germ line transmission in knockout rat production.
出版者
Nature Research
収録物名
Scientific Reports
巻(号)
6
ページ
24215
刊行年月
2016-04-11
言語
英語
ハンドルURL
https://hdl.handle.net/11094/78589
eISSN
20452322
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1038/srep24215
PMID
27062982
アクセス権
open access
権利情報
© 2016 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article