学術雑誌論文

学術雑誌論文

Scientific Reports. 2015, 5, p. 14254

Calreticulin is required for development of the cumulus oocyte complex and female fertility

Nature Research

英語

2015-09-21

https://doi.org/10.1038/srep14254

Tokuhiro, K., Satouh, Y., Nozawa, K. et al. Calreticulin is required for development of the cumulus oocyte complex and female fertility. Sci Rep 5, 14254 (2015). https://doi.org/10.1038/srep14254

Calnexin (CANX) and calreticulin (CALR) chaperones mediate nascent glycoprotein folding in the endoplasmic reticulum. Here we report that these chaperones have distinct roles in male and female fertility. Canx null mice are growth retarded but fertile. Calr null mice die during embryonic development, rendering indeterminate any effect on reproduction. Therefore, we conditionally ablated Calr in male and female germ cells using Stra8 (mcKO) and Zp3 (fcKO) promoter-driven Cre recombinase, respectively. Calr mcKO male mice were fertile, but fcKO female mice were sterile despite normal mating behavior. Strikingly, we found that Calr fcKO female mice had impaired folliculogenesis and decreased ovulatory rates due to defective proliferation of cuboidal granulosa cells. Oocyte-derived, TGF-beta family proteins play a major role in follicular development and molecular analysis revealed that the normal processing of GDF9 and BMP15 was defective in Calr fcKO oocytes. These findings highlight the importance of CALR in female reproduction and demonstrate that compromised CALR function leads to ovarian insufficiency and female infertility.

© 2015 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.

78596

Calreticulin is required for development of the cumulus oocyte complex and female fertility

Nature Research

英語

2015-09-21

Scientific Reports

5

14254

https://doi.org/10.1038/srep14254

26388295

https://hdl.handle.net/11094/78596