A novel transcriptional factor Nkapl is a germ cell-specific suppressor of Notch signaling and is indispensable for spermatogenesis

Okuda, Hidenobu; Kiuchi, Hiroshi; Takao, Tetsuya et al.

PLoS ONE, 2015, 10(4), e0124293

アクセス数:365件（2025-04-18 02:49 集計）

固定URL: https://hdl.handle.net/11094/78598

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タイトル
A novel transcriptional factor Nkapl is a germ cell-specific suppressor of Notch signaling and is indispensable for spermatogenesis
著者
著者名
Okuda, Hidenobu
NRID
1000070403053 1000070403053
著者名
Kiuchi, Hiroshi
NRID
1000030379177 1000030379177
著者名
Takao, Tetsuya
NRID
1000070362704 1000070362704
著者名
Miyagawa, Yasushi
NRID
1000040294053 1000040294053
著者名
Tsujimura, Akira
NRID
1000030263263 1000030263263
researchmap permalink
read0185096 read0185096
Scopus Author ID
7005880662 7005880662
著者名
Nonomura, Norio
NRID
1000050604732 1000050604732
researchmap permalink
hmiyata hmiyata
Scopus Author ID
37161837000 37161837000
ORCID
0000-0003-4758-5803 0000-0003-4758-5803
著者名
Miyata, Haruhiko
NRID
1000030089875 1000030089875
著者名
Okabe, Masaru
NRID
1000020304066 1000020304066
ORCID
0000-0001-9859-6217 0000-0001-9859-6217
researchmap permalink
read0054910 read0054910
Scopus Author ID
16689054500 16689054500
著者名
Ikawa, Masahito
著者名
Kawakami, Yoshitaka
NRID
1000070215482 1000070215482
著者名
Goshima, Naoki
NRID
1000020220965 1000020220965
著者名
Wada, Morimasa
NRID
1000010263310 1000010263310
著者名
Tanaka, Hiromitsu
内容
A Novel Transcriptional Factor Nkapl Is a Germ Cell-Specific Suppressor of Notch Signaling and Is Indispensable for Spermatogenesis. Okuda H, Kiuchi H, Takao T, Miyagawa Y, Tsujimura A, et al. PLOS ONE. 2015. 10(4) doi:10.1371/journal.pone.0124293
抄録
Spermatogenesis is an elaborately regulated system dedicated to the continuous production of spermatozoa via the genesis of spermatogonia. In this process, a variety of genes are expressed that are relevant to the differentiation of germ cells at each stage. Although Notch signaling plays a critical role in germ cell development in Drosophila and Caenorhabditis elegans, its function and importance for spermatogenesis in mammals is controversial. We report that Nkapl is a novel germ cell-specific transcriptional suppressor in Notch signaling. It is also associated with several molecules of the Notch corepressor complex such as CIR, HDAC3, and CSL. It was expressed robustly in spermatogonia and early spermatocytes after the age of 3 weeks. Nkapl-deleted mice showed complete arrest at the level of pachytene spermatocytes. In addition, apoptosis was observed in this cell type. Overexpression of NKAPL in germline stem cells demonstrated that Nkapl induced changes in spermatogonial stem cell (SSC) markers and the reduction of differentiation factors through the Notch signaling pathway, whereas testes with Nkapl deleted showed inverse changes in those markers and factors. Therefore, Nkapl is indispensable because aberrantly elevated Notch signaling has negative effects on spermatogenesis, affecting SSC maintenance and differentiation factors. Notch signaling should be properly regulated through the transcriptional factor Nkapl.
出版者
Public Library of Science
収録物名
PLoS ONE
巻(号)
10(4)
ページ
e0124293
刊行年月
2015-04-14
言語
英語
ハンドルURL
https://hdl.handle.net/11094/78598
eISSN
19326203
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1371/journal.pone.0124293
PMID
25875095
アクセス権
open access
権利情報
© 2015 Okuda et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article