Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span

Azuma, Kotaro; Tsukui, Tohru; Ikeda, Kazuhiro; Shiba, Sachiko; Nakagawa, Kimie; Okano, Toshio; Urano, Tomohiko; Horie, Kuniko; Ouchi, Yasuyoshi; Ikawa, Masahito; Inoue, Satoshi

doi:https://doi.org/10.1371/journal.pone.0088643

Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span

Azuma, Kotaro; Tsukui, Tohru; Ikeda, Kazuhiro et al.

PLoS ONE, 2014, 9(2), e88643

アクセス数:202件（2025-04-18 02:18 集計）

固定URL: https://hdl.handle.net/11094/78602

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
PLoSONE_09_02_e88643	pdf	なし	872 KB	105	2021.02.08

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span
著者	NRID 1000030401110 1000030401110 著者名 Azuma, Kotaro
	NRID 1000010333006 1000010333006 著者名 Tsukui, Tohru
	NRID 1000030343461 1000030343461 著者名 Ikeda, Kazuhiro
	NRID 1000070633824 1000070633824 著者名 Shiba, Sachiko
	NRID 1000090309435 1000090309435 著者名 Nakagawa, Kimie
	NRID 1000020131542 1000020131542 著者名 Okano, Toshio
	NRID 1000020334386 1000020334386 著者名 Urano, Tomohiko
	NRID 1000090261982 1000090261982 著者名 Horie, Kuniko 著者の別表記 Inoue, Kuniko
	NRID 1000080168864 1000080168864 著者名 Ouchi, Yasuyoshi
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000040251251 1000040251251 著者名 Inoue, Satoshi
内容	Liver-Specific γ-Glutamyl Carboxylase-Deficient Mice Display Bleeding Diathesis and Short Life Span. Azuma K, Tsukui T, Ikeda K, Shiba S, Nakagawa K, et al. PLOS ONE. 2014. 9(2) doi:10.1371/journal.pone.0088643
抄録	Vitamin K is a fat-soluble vitamin that plays important roles in blood coagulation and bone metabolism. One of its functions is as a co-factor for γ-glutamyl carboxylase (Ggcx). Conventional knockout of Ggcx causes death shortly after birth in homozygous mice. We created Ggcx-floxed mice by inserting loxP sequences at the sites flanking exon 6 of Ggcx. By mating these mice with albumin-Cre mice, we generated Ggcx-deficient mice specifically in hepatocytes (GgcxΔliver/Δliver mice). In contrast to conventional Ggcx knockout mice, GgcxΔliver/Δliver mice had very low activity of Ggcx in the liver and survived several weeks after birth. Furthermore, compared with heterozygous mice (Ggcx+/Δliver), GgcxΔliver/Δliver mice had shorter life spans. Ggcx Δliver/Δliver mice displayed bleeding diathesis, which was accompanied by decreased activity of coagulation factors II and IX. Ggcx-floxed mice can prove useful in examining Ggcx functions in vivo.
出版者	Public Library of Science
収録物名	PLoS ONE
巻(号)	9(2)
ページ	e88643
刊行年月	2014-02-10
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78602
eISSN	19326203
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1371/journal.pone.0088643
関連情報 (isIdenticalTo)	PMID 24520408
アクセス権	open access
権利情報	© 2014 Azuma et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	PLoS ONE. 2014, 9(2), p. e88643
DC.title	Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span
DC.creator	Azuma, Kotaro
	Tsukui, Tohru
	Ikeda, Kazuhiro
	Shiba, Sachiko
	Nakagawa, Kimie
	Okano, Toshio
	Urano, Tomohiko
	Horie, Kuniko
	Ouchi, Yasuyoshi
	Ikawa, Masahito
	Inoue, Satoshi
DC.creator	Inoue, Kuniko
DC.publisher	Public Library of Science
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2014-02-10
DC.identifier	https://doi.org/10.1371/journal.pone.0088643
DC.description	Liver-Specific γ-Glutamyl Carboxylase-Deficient Mice Display Bleeding Diathesis and Short Life Span. Azuma K, Tsukui T, Ikeda K, Shiba S, Nakagawa K, et al. PLOS ONE. 2014. 9(2) doi:10.1371/journal.pone.0088643
DCTERMS.abstract	Vitamin K is a fat-soluble vitamin that plays important roles in blood coagulation and bone metabolism. One of its functions is as a co-factor for γ-glutamyl carboxylase (Ggcx). Conventional knockout of Ggcx causes death shortly after birth in homozygous mice. We created Ggcx-floxed mice by inserting loxP sequences at the sites flanking exon 6 of Ggcx. By mating these mice with albumin-Cre mice, we generated Ggcx-deficient mice specifically in hepatocytes (GgcxΔliver/Δliver mice). In contrast to conventional Ggcx knockout mice, GgcxΔliver/Δliver mice had very low activity of Ggcx in the liver and survived several weeks after birth. Furthermore, compared with heterozygous mice (Ggcx+/Δliver), GgcxΔliver/Δliver mice had shorter life spans. Ggcx Δliver/Δliver mice displayed bleeding diathesis, which was accompanied by decreased activity of coagulation factors II and IX. Ggcx-floxed mice can prove useful in examining Ggcx functions in vivo.
DC.rights	© 2014 Azuma et al. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	78602
citation_title	Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span
citation_author	Azuma, Kotaro
	Tsukui, Tohru
	Ikeda, Kazuhiro
	Shiba, Sachiko
	Nakagawa, Kimie
	Okano, Toshio
	Urano, Tomohiko
	Horie, Kuniko
	Ouchi, Yasuyoshi
	Ikawa, Masahito
	Inoue, Satoshi
citation_publisher	Public Library of Science
citation_language	英語
citation_date	2014-02-10
citation_journal_title	PLoS ONE
citation_volume	9
citation_issue	2
citation_firstpage	e88643
citation_doi	https://doi.org/10.1371/journal.pone.0088643
citation_pmid	24520408
citation_public_url	https://hdl.handle.net/11094/78602

Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span

閲覧可能ファイル

論文情報

論文詳細を表示