Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1

Satouh, Yuhkoh; Inoue, Naokazu; Ikawa, Masahito; Okabe, Masaru

doi:https://doi.org/10.1242/jcs.100867

Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1

Satouh, Yuhkoh; Inoue, Naokazu; Ikawa, Masahito et al.

Journal of Cell Science, 2012, 125(21), 4985-4990

アクセス数:164件（2025-04-18 02:24 集計）

固定URL: https://hdl.handle.net/11094/78608

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タイトル	Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1
著者	NRID 1000040545571 1000040545571 著者名 Satouh, Yuhkoh
	NRID 1000050379096 1000050379096 著者名 Inoue, Naokazu
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	NRID 1000030089875 1000030089875 著者名 Okabe, Masaru
キーワード等	Acrosome reaction
	IZUMO1
	Live-cell imaging
	Membrane fusion
	Protein trafficking
	Sperm-egg fusion
抄録	Gene disruption experiments have proven that the acrosomal protein IZUMO1 is essential for sperm-egg fusion in the mouse. However, despite its predicted function, it is not expressed on the surface of ejaculated spermatozoa. Here, we report the dynamics of diffusion of IZUMO1 from the acrosomal membrane to the sperm surface at the time of the acrosome reaction, visualized using a fluorescent protein tag. IZUMO1 showed a tendency to localize in the equatorial segment of the sperm surface after the acrosome reaction. This region is considered to initiate fusion with the oolemma. The moment of sperm-egg fusion and the dynamic movements of proteins during fusion were also imaged live. Translocation of IZUMO1 during the fertilization process was clarified, and a fundamental mechanism in mammalian fertilization is postulated.
出版者	Company of Biologists Ltd
収録物名	Journal of Cell Science
巻(号)	125(21)
ページ	4985-4990
刊行年月	2012-11-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78608
PISSN	00219533
eISSN	14779137
NCID	AA00694823
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1242/jcs.100867
関連情報 (isIdenticalTo)	PMID 22946049
アクセス権	open access
権利情報	© 2012. Published by The Company of Biologists Ltd.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Cell Science. 2012, 125(21), p. 4985-4990
DC.title	Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1
DC.creator	Satouh, Yuhkoh
	Inoue, Naokazu
	Ikawa, Masahito
	Okabe, Masaru
DC.publisher	Company of Biologists Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2012-11-01
DC.identifier	https://doi.org/10.1242/jcs.100867
DC.subject	Acrosome reaction
	IZUMO1
	Live-cell imaging
	Membrane fusion
	Protein trafficking
	Sperm-egg fusion
DCTERMS.abstract	Gene disruption experiments have proven that the acrosomal protein IZUMO1 is essential for sperm-egg fusion in the mouse. However, despite its predicted function, it is not expressed on the surface of ejaculated spermatozoa. Here, we report the dynamics of diffusion of IZUMO1 from the acrosomal membrane to the sperm surface at the time of the acrosome reaction, visualized using a fluorescent protein tag. IZUMO1 showed a tendency to localize in the equatorial segment of the sperm surface after the acrosome reaction. This region is considered to initiate fusion with the oolemma. The moment of sperm-egg fusion and the dynamic movements of proteins during fusion were also imaged live. Translocation of IZUMO1 during the fertilization process was clarified, and a fundamental mechanism in mammalian fertilization is postulated.
DC.rights	© 2012. Published by The Company of Biologists Ltd.
DC.format	78608
citation_title	Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1
citation_author	Satouh, Yuhkoh
	Inoue, Naokazu
	Ikawa, Masahito
	Okabe, Masaru
citation_publisher	Company of Biologists Ltd
citation_language	英語
citation_date	2012-11-01
citation_journal_title	Journal of Cell Science
citation_volume	125
citation_issue	21
citation_firstpage	4985
citation_lastpage	4990
citation_doi	https://doi.org/10.1242/jcs.100867
citation_pmid	22946049
citation_public_url	https://hdl.handle.net/11094/78608

Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1

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