Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse

Suga, Tomohiro; Kimura, En; Morioka, Yuka; Ikawa, Masahito; Li, Sheng; Uchino, Katsuhisa; Uchida, Yuji; Yamashita, Satoshi; Maeda, Yasushi; Chamberlain, Jeffrey S.; Uchino, Makoto

doi:https://doi.org/10.1371/journal.pone.0016908

Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse

Suga, Tomohiro; Kimura, En; Morioka, Yuka et al.

PLoS ONE, 2011, 6(3), e16908

アクセス数:295件（2025-04-18 02:03 集計）

固定URL: https://hdl.handle.net/11094/78612

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タイトル	Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse
著者	NRID 1000090613521 1000090613521 著者名 Suga, Tomohiro
	NRID 1000060433025 1000060433025 著者名 Kimura, En
	NRID 1000000360264 1000000360264 著者名 Morioka, Yuka
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
	著者名 Li, Sheng
	著者名 Uchino, Katsuhisa
	NRID 1000070433026 1000070433026 著者名 Uchida, Yuji
	NRID 1000020457592 1000020457592 著者名 Yamashita, Satoshi
	NRID 1000060346997 1000060346997 著者名 Maeda, Yasushi
	著者名 Chamberlain, Jeffrey S.
	NRID 1000020117336 1000020117336 著者名 Uchino, Makoto
内容	Muscle Fiber Type-Predominant Promoter Activity in Lentiviral-Mediated Transgenic Mouse. Suga T, Kimura E, Morioka Y, Ikawa M, Li S, Uchino K, et al. PLOS ONE. 2011. 6(3) doi:10.1371/journal.pone.0016908
抄録	Variations in gene promoter/enhancer activity in different muscle fiber types after gene transduction was noticed previously, but poorly analyzed. The murine stem cell virus (MSCV) promoter drives strong, stable gene expression in hematopoietic stem cells and several other cells, including cerebellar Purkinje cells, but it has not been studied in muscle. We injected a lentiviral vector carrying an MSCV-EGFP cassette (LvMSCV-EGFP) into tibialis anterior muscles and observed strong EGFP expression in muscle fibers, primary cultured myoblasts, and myotubes isolated from injected muscles. We also generated lentiviral-mediated transgenic mice carrying the MSCV-EGFP cassette and detected transgene expression in striated muscles. LvMSCV-EGFP transgenic mice showed fiber type-dependent variations in expression: highest in types I and IIA, intermediate in type IID/X, and lowest in type IIB fibers. The soleus and diaphragm muscles, consisting mainly of types I and IIA, are most severely affected in the mdx mouse model of muscular dystrophy. Further analysis of this promoter may have the potential to achieve certain gene expression in severely affected muscles of mdx mice. The Lv-mediated transgenic mouse may prove a useful tool for assessing the enhancer/promoter activities of a variety of different regulatory cassettes.
出版者	Public Library of Science
収録物名	PLoS ONE
巻(号)	6(3)
ページ	e16908
刊行年月	2011-03-18
言語	英語
ハンドルURL	https://hdl.handle.net/11094/78612
eISSN	19326203
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1371/journal.pone.0016908
関連情報 (isIdenticalTo)	PMID 21445245
アクセス権	open access
権利情報	© 2011 Suga et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	PLoS ONE. 2011, 6(3), p. e16908
DC.title	Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse
DC.creator	Suga, Tomohiro
	Kimura, En
	Morioka, Yuka
	Ikawa, Masahito
	Li, Sheng
	Uchino, Katsuhisa
	Uchida, Yuji
	Yamashita, Satoshi
	Maeda, Yasushi
	Chamberlain, Jeffrey S.
	Uchino, Makoto
DC.publisher	Public Library of Science
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2011-03-18
DC.identifier	https://doi.org/10.1371/journal.pone.0016908
DC.description	Muscle Fiber Type-Predominant Promoter Activity in Lentiviral-Mediated Transgenic Mouse. Suga T, Kimura E, Morioka Y, Ikawa M, Li S, Uchino K, et al. PLOS ONE. 2011. 6(3) doi:10.1371/journal.pone.0016908
DCTERMS.abstract	Variations in gene promoter/enhancer activity in different muscle fiber types after gene transduction was noticed previously, but poorly analyzed. The murine stem cell virus (MSCV) promoter drives strong, stable gene expression in hematopoietic stem cells and several other cells, including cerebellar Purkinje cells, but it has not been studied in muscle. We injected a lentiviral vector carrying an MSCV-EGFP cassette (LvMSCV-EGFP) into tibialis anterior muscles and observed strong EGFP expression in muscle fibers, primary cultured myoblasts, and myotubes isolated from injected muscles. We also generated lentiviral-mediated transgenic mice carrying the MSCV-EGFP cassette and detected transgene expression in striated muscles. LvMSCV-EGFP transgenic mice showed fiber type-dependent variations in expression: highest in types I and IIA, intermediate in type IID/X, and lowest in type IIB fibers. The soleus and diaphragm muscles, consisting mainly of types I and IIA, are most severely affected in the mdx mouse model of muscular dystrophy. Further analysis of this promoter may have the potential to achieve certain gene expression in severely affected muscles of mdx mice. The Lv-mediated transgenic mouse may prove a useful tool for assessing the enhancer/promoter activities of a variety of different regulatory cassettes.
DC.rights	© 2011 Suga et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DC.format	78612
citation_title	Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse
citation_author	Suga, Tomohiro
	Kimura, En
	Morioka, Yuka
	Ikawa, Masahito
	Li, Sheng
	Uchino, Katsuhisa
	Uchida, Yuji
	Yamashita, Satoshi
	Maeda, Yasushi
	Chamberlain, Jeffrey S.
	Uchino, Makoto
citation_publisher	Public Library of Science
citation_language	英語
citation_date	2011-03-18
citation_journal_title	PLoS ONE
citation_volume	6
citation_issue	3
citation_firstpage	e16908
citation_doi	https://doi.org/10.1371/journal.pone.0016908
citation_pmid	21445245
citation_public_url	https://hdl.handle.net/11094/78612

Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse

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