学術雑誌論文

学術雑誌論文

text

The Journal of Physical Chemistry B. 2021, 125(8), p. 2089-2097

Glycan Cluster Shielding and Antibody Epitopes on Lassa Virus Envelop Protein

American Chemical Society

英語

text

2021-02-19

https://doi.org/10.1021/acs.jpcb.0c11516

This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry B, ©2021 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jpcb.0c11516.

An understanding of how an antiviral monoclonal antibody recognizes its target is vital for the development of neutralizing antibodies and vaccines. The extensive glycosylation of viral proteins almost certainly affects the antibody response, but the investigation of such effects is hampered by the huge range of structures and interactions of surface glycans through their inherent complexity and flexibility. Here, we built an atomistic model of a fully glycosylated envelope protein complex of the Lassa virus and performed molecular dynamics simulations to characterize the impact of surface glycans on the antibody response. The simulations attested to the variety of conformations and interactions of surface glycans. The results show that glycosylation nonuniformly shields the surface of the complex and only marginally affects protein dynamics. The glycans gather in distinct clusters through interaction with protein residues, and only a few regions are left accessible by an antibody. We successfully recovered known protein epitopes by integrating the simulation results with existing sequence- and structure-based epitope prediction methods. The results emphasize the rich structural environment of glycans and demonstrate that shielding is not merely envelopment by a uniform blanket of sugars. This work provides a molecular basis for integrating otherwise elusive structural properties of glycans into vaccine and neutralizing antibody developments.

Copyright © 2021 American Chemical Society

78921

Glycan Cluster Shielding and Antibody Epitopes on Lassa Virus Envelop Protein

American Chemical Society

英語

2021-02-19

The Journal of Physical Chemistry B

125

8

2089

2097

https://doi.org/10.1021/acs.jpcb.0c11516

https://hdl.handle.net/11094/78921